FastQCFastQC Report
Fri 10 Feb 2017
ERR1632665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427701
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA35770.2505426556400815No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA28310.19829081859577039No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA21870.1531833346057753No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19290.13511232393897601No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18890.13231061685885212No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT18240.12775784285365072No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT17860.12509622112753302No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16700.11697127059517363No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15920.11150794178893199No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15880.11122777108091961No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC15670.10975687486385455No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15300.10716529581473994No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14300.10016102811443012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC2250.029.629
GGTATCA16100.029.0714261
TACGGCT2900.024.24137930
CTAGACT400.001931414723.1250024
GCGGTAA15150.020.27062823
AAACACG1750.020.08571415
GACCGTT1500.019.7333347
GGACGTA1600.019.6562527
AACTCCG16000.019.656255
GTATCAA23850.019.547172
TATACAC4550.019.51648337
TTTTACG951.6768172E-719.4736844
GGTAATA16250.019.35384825
CGTGCCA16500.019.17272810
ACACGAA1650.019.06060417
CGCGGTA16700.018.72155822
AGCCGCG16900.018.60946819
TAATACG14950.018.56187227
TCTATAC500.007035696418.53
CGGTAAT16600.018.49999824
Produced by FastQC (version 0.11.5)