FastQCFastQC Report
Fri 10 Feb 2017
ERR1632664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCATTTAGGTTAGAATGTGCACAGGCATTGTACCA21.4492753623188406No Hit
GATTGTAATGGGTATGGAGACATATCATATAAGTAATGCTAGG10.7246376811594203No Hit
ACCCATTACAGCCCGATCTCCGACCGCGAGCGCGCCGCCCGCC10.7246376811594203No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTT10.7246376811594203No Hit
ACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCC10.7246376811594203No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC10.7246376811594203No Hit
CATGTGGTGTATACATCGGGGTAGTCCGAGTAACGTCGGGGCA10.7246376811594203No Hit
CGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGG10.7246376811594203No Hit
CCCTTGGACAAGACAAACCAAGTCGGAACAGCAGATAACCTGT10.7246376811594203No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGCTGTC10.7246376811594203No Hit
TCTTCACTGCACCCTGGATTTGCATACATTCTTCAAGATCCCA10.7246376811594203No Hit
TAAACACACAGTGGCAATTCATGGCATTTGGTAATTCGTCCAG10.7246376811594203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10.7246376811594203No Hit
GCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTT10.7246376811594203No Hit
GGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACCTGT10.7246376811594203No Hit
GAATTGAAGCCCCGGTAAACGGCGGCCGTAACGATAACGGTCC10.7246376811594203No Hit
GTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATATGGTTA10.7246376811594203No Hit
AGTCTGAATCACTGTTAGAAAATAAAATCCAGGTTAGAGAAAA10.7246376811594203No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA10.7246376811594203No Hit
GTTCCACTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCT10.7246376811594203No Hit
AGTTAGCCTGAGTTGACTCTCATTGTTCCCCTATCTACCCACA10.7246376811594203No Hit
TGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATC10.7246376811594203No Hit
GTGGTGACGCGTCTTCGCCCATATGCATCTGCAGCGGTGGTGA10.7246376811594203No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGCTGTCTCTTATAC10.7246376811594203No Hit
ATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGTCTCTTA10.7246376811594203No Hit
CCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCT10.7246376811594203No Hit
CCTTATGAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAA10.7246376811594203No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT10.7246376811594203No Hit
GGAAGATGGTGATGGGATTTCCATTGATGACAAGCTTCCCGTT10.7246376811594203No Hit
CTTTCATACAGGGCAGCCACACCTTGTCCCTGTACCTGTCTCT10.7246376811594203No Hit
CTTATGTTGTGGAACCTGAAACATAACCACGTGTTTTCTTATA10.7246376811594203No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGCTGTCTCT10.7246376811594203No Hit
TTCCTAGAGCTTTCACTCACTCATCTTAGCTTTATCCCTCGTG10.7246376811594203No Hit
ACCCACGAAGCCGAACTCCTCGGCGAAGGTCGCGAAGATGAAG10.7246376811594203No Hit
TTGCTGGAGCAGCTGAACGAGCAGTTTAACTGGGTGTCCCGGC10.7246376811594203No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGCTGTC10.7246376811594203No Hit
CCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCG10.7246376811594203No Hit
TTTTTGCCCAGAGAGCAAGTTTATTTGGTGAATGCTGACGGCA10.7246376811594203No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC10.7246376811594203No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAACTGT10.7246376811594203No Hit
GCCGAGCCGGGTGCGCACGGGGAGGCGGAGGCAGCGCTGTCTC10.7246376811594203No Hit
ACTAAAGATGACGGAGGCAAAAAAAAAAAAAAAAAAAAAAAAA10.7246376811594203No Hit
GGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCA10.7246376811594203No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10.7246376811594203No Hit
GGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAACTGTC10.7246376811594203No Hit
GATAAGATTCCTTTACTACAGCAGCCAAAACGAGAAGAAGAAG10.7246376811594203No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA10.7246376811594203No Hit
CCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTT10.7246376811594203No Hit
CCCTACACCTGTGTCAGCTGCGGTGCCCGGTACTGCACCTGTC10.7246376811594203No Hit
CCCCAGCCCCGCTCACGCCAGTCCCTCTTCCTCTGCCGGGAGG10.7246376811594203No Hit
GGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAAT10.7246376811594203No Hit
TTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCC10.7246376811594203No Hit
GGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG10.7246376811594203No Hit
GCATAAGCAGCTGGCAGAGGCAAGGAAAGACCAGCAGAGAGAA10.7246376811594203No Hit
GTTAACAGCAATCAGAAGAGATGGGGGCAGGCACACCTGTCAG10.7246376811594203No Hit
GGTGATGGTCAAGTAAACTATGAAGAGTTTGTACAAATGATGA10.7246376811594203No Hit
GATCCGGGGAGCGGTGTCATTGTAACTCACAGGAGGATGGATC10.7246376811594203No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG10.7246376811594203No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAG10.7246376811594203No Hit
GCTGAGGCCGAGCGCCTTCGCCAGATCATCAAGGACTGTCTCT10.7246376811594203No Hit
GTGGAACCCACTTCCTGCCAACAATGATGGCCTTACTAAACAG10.7246376811594203No Hit
GCTCTCGTTCACCGACAGCGAGGAATCGCCGCCCGCCACCGGG10.7246376811594203No Hit
GAGACAGAGTGAGAGAGTGTGTATGTATGTGTGTGGTGTGTGT10.7246376811594203No Hit
CCCTTGAGCCCCCTCGCTTCCCGACGTTCCGTTCCCCCTGTCT10.7246376811594203No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10.7246376811594203No Hit
GGCAGAAGCTTTTTGCAGAGCCCAGTGGGAGACTCAGATAAGC10.7246376811594203No Hit
GCCTGGCTCTGGTATCACCGGGTGGTGGGCGAGAACCGTTGCC10.7246376811594203No Hit
GCTTTACTACTCTTTTGTGAGGAGTACATGTTATGCATATTAA10.7246376811594203No Hit
CTTAGAGGCTTTTCCTGGAAGCAGGGCATTTGTTGCTTCAGCA10.7246376811594203No Hit
GTATCAACGCAGAGTACGGGAGAGGATGACCAGCCACACTGGA10.7246376811594203No Hit
GTCTCTGCTAGTGTGGAGATAAATCATATTATGGCCAAGGGTC10.7246376811594203No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT10.7246376811594203RNA PCR Primer, Index 46 (95% over 21bp)
CAGTGGTAGTGTTTCAGAGGCCAGAGTCGCGACGGCCATCTTC10.7246376811594203No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT10.7246376811594203No Hit
CGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATTACCCAAT10.7246376811594203No Hit
AGGCAGATGATGCAGAGGAGCGAGCTGAGCGCCTCCAGCGAGA10.7246376811594203No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT10.7246376811594203No Hit
CGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCCTG10.7246376811594203No Hit
GAGCATGGTGGCATGCGCCTGTATTCCCAGCCAGTAGTCCCAC10.7246376811594203No Hit
AGGCAAGACCTAGAGTTCAAAATTACATGATCTCCATCTTAAA10.7246376811594203No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10.7246376811594203No Hit
CAACCTCTCCTTTGTAGAATTTTTCTTTGTTAGAAAACAGACA10.7246376811594203No Hit
CACCGGAACTGGCGCCCCGGTTTCATAGCCTCCCACCTATCCT10.7246376811594203No Hit
CGCTCGCGCACGATGCGGTAGGCGTCCTCGTCACTGTCTCTTA10.7246376811594203No Hit
CTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATT10.7246376811594203No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA10.7246376811594203No Hit
CTTTTGGAGGTGCTTCTCATGCAAAAGGAATCGTGCTGGAACT10.7246376811594203No Hit
GACTTCTAACATAGCATATGAGGTGAAAACACTGCTTTAGTAA10.7246376811594203No Hit
GATCACACATGATTATGATCTAATTCAAGCCAATCTTCTCAAG10.7246376811594203No Hit
CTGTACACCTGTCCCCCTGCTTCAGGGACGTCCAGATCGGTGA10.7246376811594203No Hit
CTGATAGAGAGGTAGGAGGCACGTTCTTGGTCACTGTTCCATT10.7246376811594203No Hit
ATTTACGGATAAATTTAGTGAAAGTGCAATCAATACATTATTT10.7246376811594203No Hit
CCGGGGCTTCGATCAAGAGCTTCGCTTGCGCTGACCCCATCAA10.7246376811594203No Hit
GCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACC10.7246376811594203No Hit
CTCCCTCTACCAGCTGGAGAACTACTGCAACTAGACGCAGCCC10.7246376811594203No Hit
TAATTGTGCTTAAGGCTTTAATTAAATTTGGGGGTTCCCTTCT10.7246376811594203No Hit
CTGTGAAGATGTAGTGTACCCGCGGCAAGACGGAAAGACCCCG10.7246376811594203No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA10.7246376811594203No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTT10.7246376811594203No Hit
GGCTGCTTTCATTGGGCAGGTCTGGTGGTTACATAGCTGGGCA10.7246376811594203No Hit
CTTATAATATGCAAATCACAATGTATATAAGTTATCTAGAGAT10.7246376811594203No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA10.7246376811594203No Hit
CCCTATACGTCATCTTGCGATTTCGCAGAGCCCTGTGTTTTTG10.7246376811594203No Hit
TTCTTATTCACCATTGTAACTCATCTCCTTTCCCTACCCAGTG10.7246376811594203No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACT10.7246376811594203No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA10.7246376811594203No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC10.7246376811594203No Hit
AGTTAATAGAAATTGAACCAGAGTTTTCTTATGTTTGCTTGAA10.7246376811594203No Hit
GTGTATATTAATGAAACTGAAGAATAAATTTCATTTGATTATA10.7246376811594203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7246376811594203No Hit
TAGTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGAT10.7246376811594203No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA10.7246376811594203No Hit
GGCCGAGGCCGTTCCGAACACTGTCACATTCTCCACCTGTCTC10.7246376811594203No Hit
TTTACTTACTGTATGAGCCGATCTGAATCCATTTTCAGTTACT10.7246376811594203No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTG10.7246376811594203No Hit
TTCTGGAAAACTGCACAGAGAATTCCATCATGGACCTGACCCA10.7246376811594203No Hit
GGCCCCAGCGCCCGAGGTCTACGCGGGCCGCCCGCTGCTGCCA10.7246376811594203No Hit
TCACGTACCTGTGCAGAAACCGCCTCTGTGGCTGCATTTGAAA10.7246376811594203No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT10.7246376811594203No Hit
AGCGTGGACTTACTCCCTTTTGTGCAGAAGGCCCCCAAAGACA10.7246376811594203No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG10.7246376811594203No Hit
GATGCACTTGGTCAGGTCGATGTCGTAGCGCGAGGTGCGACGC10.7246376811594203No Hit
GAGGAGAAGTCCCTCATTCCTTGGGATTGGTGACGACAGCCGT10.7246376811594203No Hit
TCTAGAGACAGAGACTGAGTCACTGGCCCATCTCTTTGCTCTT10.7246376811594203No Hit
CTGGGTGACAGAGTGAGACCATCACAAATAAATAAATAAATAA10.7246376811594203No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT10.7246376811594203No Hit
TATCAACGCAGAGTACGGGGTACCGTGAGGGAAAGGCGAAAAA10.7246376811594203No Hit
GCTTTGAATCTGCTGGATTGGTCTCCCCAAGCATCACTCGGCC10.7246376811594203No Hit
GGACAACTAAGAAGCTATGGTAGCAAACAGTGATGTTGATGGA10.7246376811594203No Hit
CAGTGTACCCGCGGCAAGACGGAAAGACCCCGTGAACCTTTAC10.7246376811594203No Hit
CCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGTCGACTCAC10.7246376811594203No Hit
ATCGAATAGCCCAAGGAGGGTGACGGAGATGCCCACAGCAGGA10.7246376811594203No Hit
ATCCATTTCACTATCATATTCATCGGCGTAAATCTAACCTTCT10.7246376811594203No Hit
TTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCC10.7246376811594203No Hit
GACCTGTGTTGAGTTACAAAATTGTTCTTACCACTTTCTGTGA10.7246376811594203No Hit
GTGGTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGCTGTC10.7246376811594203No Hit
CCCCAGGGGGCAACTGACATTGCTCCAAGATGTTCTCCTGATG10.7246376811594203No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers