FastQCFastQC Report
Fri 10 Feb 2017
ERR1632663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12505
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT630.5037984806077569RNA PCR Primer, Index 27 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA410.32786885245901637No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA400.3198720511795282No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA360.2878848460615754No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT240.1919232307077169No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.1919232307077169No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA230.1839264294282287No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA200.1599360255897641No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC190.1519392243102759No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC190.1519392243102759No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC190.1519392243102759RNA PCR Primer, Index 27 (95% over 24bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA180.1439424230307877No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT170.13594562175129948No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT170.13594562175129948No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA160.12794882047181128No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC160.12794882047181128No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT150.11995201919232307No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA150.11995201919232307No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC150.11995201919232307No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA150.11995201919232307No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG150.11995201919232307No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA150.11995201919232307No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA150.11995201919232307No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC140.11195521791283486No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA130.10395841663334666No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC130.10395841663334666No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG130.10395841663334666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC200.001773174637.015
TACGGAG200.001773174637.030
GCGGTAA200.001773174637.023
ATACGGA200.001773174637.029
CGCGGTA200.001773174637.022
CGTGCCA200.001773174637.010
TAATACG200.001773174637.027
TCCGTGC200.001773174637.08
AATACGG200.001773174637.028
GTAATAC200.001773174637.026
CTCCGTG200.001773174637.07
CGGTAAT200.001773174637.024
GGTAATA200.001773174637.025
CCGCGGT200.001773174637.021
CCGTGCC250.00529345729.5999989
GCCAGCA250.00529345729.59999813
AACTCCG250.00529345729.5999985
TAACTCC250.00529345729.5999984
CTAACTC250.00529345729.5999983
GCTAACT250.00529345729.5999982
Produced by FastQC (version 0.11.5)