##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12505
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02734906037585	34.0	33.0	34.0	31.0	34.0
2	33.17105157936825	34.0	33.0	34.0	31.0	34.0
3	33.244702119152336	34.0	34.0	34.0	31.0	34.0
4	36.52722910835666	37.0	37.0	37.0	35.0	37.0
5	36.498680527788885	37.0	37.0	37.0	35.0	37.0
6	36.53970411835266	37.0	37.0	37.0	35.0	37.0
7	36.54762095161935	37.0	37.0	37.0	35.0	37.0
8	36.53658536585366	37.0	37.0	37.0	35.0	37.0
9	38.33018792483007	39.0	39.0	39.0	37.0	39.0
10	38.3234706117553	39.0	39.0	39.0	37.0	39.0
11	38.43334666133546	39.0	39.0	39.0	37.0	39.0
12	38.337385045981605	39.0	39.0	39.0	37.0	39.0
13	38.414234306277486	39.0	39.0	39.0	37.0	39.0
14	39.92315073970412	41.0	40.0	41.0	38.0	41.0
15	39.943302678928426	41.0	40.0	41.0	38.0	41.0
16	39.92674930027989	41.0	40.0	41.0	38.0	41.0
17	39.91811275489804	41.0	40.0	41.0	38.0	41.0
18	39.94874050379848	41.0	40.0	41.0	38.0	41.0
19	39.943142742902836	41.0	40.0	41.0	38.0	41.0
20	39.90691723310676	41.0	40.0	41.0	38.0	41.0
21	39.855897640943624	41.0	40.0	41.0	38.0	41.0
22	39.84966013594562	41.0	40.0	41.0	38.0	41.0
23	39.831347461015596	41.0	40.0	41.0	38.0	41.0
24	39.79320271891243	41.0	40.0	41.0	38.0	41.0
25	39.77201119552179	41.0	40.0	41.0	38.0	41.0
26	39.67996801279488	41.0	40.0	41.0	37.0	41.0
27	39.575449820071974	41.0	40.0	41.0	37.0	41.0
28	39.535865653738504	41.0	40.0	41.0	37.0	41.0
29	39.48188724510196	41.0	40.0	41.0	37.0	41.0
30	39.450699720111956	41.0	39.0	41.0	37.0	41.0
31	39.3859256297481	41.0	39.0	41.0	36.0	41.0
32	39.32483006797281	41.0	39.0	41.0	36.0	41.0
33	39.21359456217513	41.0	39.0	41.0	35.0	41.0
34	39.1531387445022	41.0	39.0	41.0	35.0	41.0
35	39.08988404638145	41.0	39.0	41.0	35.0	41.0
36	39.030067972810876	41.0	39.0	41.0	35.0	41.0
37	38.967213114754095	41.0	39.0	41.0	35.0	41.0
38	38.87788884446221	40.0	39.0	41.0	35.0	41.0
39	38.7483406637345	40.0	38.0	41.0	35.0	41.0
40	38.74402239104358	40.0	38.0	41.0	35.0	41.0
41	38.680847660935626	40.0	38.0	41.0	35.0	41.0
42	38.606237504998	40.0	38.0	41.0	35.0	41.0
43	37.86157536985206	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	7.0
24	6.0
25	12.0
26	16.0
27	17.0
28	29.0
29	46.0
30	48.0
31	64.0
32	100.0
33	138.0
34	201.0
35	300.0
36	519.0
37	949.0
38	2449.0
39	7601.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.21471411435426	15.985605757696922	12.626949220311875	28.172730907636947
2	21.82327069172331	19.128348660535785	30.339864054378246	28.708516593362653
3	21.647341063574572	19.32826869252299	27.532986805277886	31.49140343862455
4	16.145541783286685	14.39424230307877	33.59456217512995	35.8656537385046
5	18.464614154338264	32.051179528188726	32.115153938424626	17.36905237904838
6	35.601759296281486	33.4266293482607	14.826069572171132	16.145541783286685
7	31.163534586165532	26.14954018392643	19.200319872051182	23.486605357856856
8	27.756897241103555	29.0843662534986	19.928028788484607	23.230707716913233
9	27.708916433426626	12.219112355057977	17.41703318672531	42.654938024790084
10	19.11235505797681	22.80687724910036	28.604558176729306	29.476209516193524
11	41.41543382646941	18.280687724910035	17.41703318672531	22.886845261895242
12	23.158736505397844	23.614554178328667	25.73370651739304	27.49300279888045
13	36.593362654938026	16.457417033186726	19.616153538584566	27.333066773290682
14	24.870051979208316	18.97640943622551	21.303478608556578	34.8500599760096
15	30.267892842862853	23.87844862055178	18.920431827269095	26.933226709316273
16	27.237105157936824	22.247101159536186	22.071171531387446	28.44462215113954
17	27.724910035985605	22.662934826069574	21.111555377848862	28.500599760095962
18	26.805277888844465	21.183526589364256	22.58296681327469	29.428228708516595
19	29.332267093162734	21.95921631347461	22.614954018392645	26.093562574970015
20	31.123550579768093	20.391843262694923	21.175529788084766	27.309076369452217
21	28.276689324270293	21.6953218712515	21.29548180727709	28.732506997201117
22	28.556577369052377	21.663334666133547	20.551779288284685	29.22830867652939
23	29.46821271491403	20.967612954818073	21.33546581367453	28.228708516593365
24	29.18032786885246	20.799680127948818	21.039584166333466	28.98040783686525
25	29.372251099560177	20.7437025189924	21.679328268692526	28.2047181127549
26	29.53218712514994	21.679328268692526	21.271491403438624	27.516993202718915
27	27.588964414234308	21.815273890443823	22.93482606957217	27.6609356257497
28	27.357057177129146	21.9672131147541	21.647341063574572	29.028388644542186
29	27.652938824470212	23.29468212714914	20.975609756097562	28.07676929228309
30	27.740903638544584	21.24750099960016	23.014794082367054	27.996801279488203
31	28.692522990803678	21.527389044382247	21.759296281487405	28.020791683326667
32	27.31707317073171	20.735705717712914	21.887245101959216	30.059976009596163
33	27.061175529788084	21.2874850059976	23.158736505397844	28.49260295881647
34	27.43702518992403	21.19952019192323	22.654938024790084	28.708516593362653
35	26.54938024790084	22.990803678528586	23.478608556577367	26.981207516993205
36	27.237105157936824	20.89564174330268	24.390243902439025	27.47700919632147
37	27.277089164334267	20.319872051179527	23.958416633346662	28.44462215113954
38	26.381447421031588	20.807676929228307	24.76609356257497	28.04478208716513
39	26.557377049180324	19.704118352658938	25.605757696921234	28.132746901239504
40	25.605757696921234	20.247900839664133	27.09316273490604	27.0531787285086
41	24.470211915233904	19.584166333466612	27.101159536185527	28.844462215113953
42	22.62295081967213	20.999600159936023	29.252299080367855	27.12514994002399
43	21.47141143542583	21.5593762495002	27.9328268692523	29.036385445821672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	3.0
14	5.0
15	8.5
16	12.0
17	10.5
18	9.0
19	9.0
20	11.0
21	13.0
22	10.0
23	7.0
24	12.5
25	18.0
26	18.0
27	19.5
28	21.0
29	28.0
30	35.0
31	44.5
32	54.0
33	54.0
34	84.0
35	114.0
36	137.0
37	160.0
38	187.0
39	214.0
40	214.0
41	280.0
42	346.0
43	412.0
44	478.0
45	609.5
46	741.0
47	741.0
48	859.0
49	977.0
50	1101.5
51	1226.0
52	1276.5
53	1327.0
54	1327.0
55	1295.5
56	1264.0
57	1240.5
58	1217.0
59	1192.5
60	1168.0
61	1168.0
62	1066.5
63	965.0
64	809.0
65	653.0
66	572.5
67	492.0
68	492.0
69	418.0
70	344.0
71	311.5
72	279.0
73	212.5
74	146.0
75	146.0
76	117.5
77	89.0
78	69.5
79	50.0
80	47.0
81	44.0
82	44.0
83	29.0
84	14.0
85	12.5
86	11.0
87	9.0
88	7.0
89	7.0
90	5.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12505.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.62375049980008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.30132910136878	73.61055577768893
2	4.632017456853799	7.469012395041983
3	1.5671493751239833	3.7904838064774093
4	0.813330688355485	2.622950819672131
5	0.42650267804007147	1.7193122750899639
6	0.3769093433842492	1.8232706917233106
7	0.16861733782979568	0.9516193522590964
8	0.14878000396746677	0.9596161535385845
9	0.10910533624280898	0.7916833266693324
>10	0.44634001190240036	5.757696921231507
>50	0.00991866693116445	0.5037984806077569
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	63	0.5037984806077569	RNA PCR Primer, Index 27 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	41	0.32786885245901637	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	40	0.3198720511795282	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	36	0.2878848460615754	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	24	0.1919232307077169	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	24	0.1919232307077169	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	23	0.1839264294282287	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	20	0.1599360255897641	No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	19	0.1519392243102759	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	19	0.1519392243102759	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	19	0.1519392243102759	RNA PCR Primer, Index 27 (95% over 24bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	18	0.1439424230307877	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	17	0.13594562175129948	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	17	0.13594562175129948	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	16	0.12794882047181128	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	16	0.12794882047181128	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	15	0.11995201919232307	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	15	0.11995201919232307	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	15	0.11995201919232307	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	15	0.11995201919232307	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	15	0.11995201919232307	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	15	0.11995201919232307	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	15	0.11995201919232307	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	14	0.11195521791283486	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	13	0.10395841663334666	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	13	0.10395841663334666	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	13	0.10395841663334666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.01599360255897641	0.0
27	0.0	0.0	0.0	0.04798080767692923	0.0
28	0.0	0.0	0.0	0.10395841663334666	0.0
29	0.0	0.0	0.0	0.23190723710515793	0.0
30	0.0	0.0	0.0	0.3678528588564574	0.0
31	0.0	0.0	0.0	0.7916833266693323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCAGC	20	0.0017731746	37.0	15
TACGGAG	20	0.0017731746	37.0	30
GCGGTAA	20	0.0017731746	37.0	23
ATACGGA	20	0.0017731746	37.0	29
CGCGGTA	20	0.0017731746	37.0	22
CGTGCCA	20	0.0017731746	37.0	10
TAATACG	20	0.0017731746	37.0	27
TCCGTGC	20	0.0017731746	37.0	8
AATACGG	20	0.0017731746	37.0	28
GTAATAC	20	0.0017731746	37.0	26
CTCCGTG	20	0.0017731746	37.0	7
CGGTAAT	20	0.0017731746	37.0	24
GGTAATA	20	0.0017731746	37.0	25
CCGCGGT	20	0.0017731746	37.0	21
CCGTGCC	25	0.005293457	29.599998	9
GCCAGCA	25	0.005293457	29.599998	13
AACTCCG	25	0.005293457	29.599998	5
TAACTCC	25	0.005293457	29.599998	4
CTAACTC	25	0.005293457	29.599998	3
GCTAACT	25	0.005293457	29.599998	2
>>END_MODULE