FastQCFastQC Report
Fri 10 Feb 2017
ERR1632662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583182
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA34030.21494686018411024No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA33810.21355725368277303No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24830.1568360428554645No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA23010.14534020725349328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20920.13213894549079008No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20700.13074933898945287No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT20600.13011769967066325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.11982197877439234No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17530.1107263725838217No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17220.10876829069557385No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17130.1081998153086632No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16980.10725235633047875No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16400.10358884828149892No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16370.10339935648586202No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16340.10320986469022514No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16110.10175709425700899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.027.8558351
ATACGGC1800.026.72222129
TCGACAG358.8708807E-426.428575
TACGGCT2100.024.66666630
TAGTCGG451.3235206E-424.66666630
GCGTTAT502.7027263E-422.21
GTGTTAT603.72743E-521.5833341
TCGCTAA450.00382643720.55555514
CACTCTA1403.6379788E-1219.8214289
CTTATAC21200.019.37264337
GTATCAA32200.018.7872682
TTCTAGC701.21956626E-418.52
ACTCTAA1409.458745E-1118.510
GTACTAC609.2389144E-418.51
TACCGCT1103.8542566E-818.535
GCGGTAA16850.017.89614123
GGTAATA17050.017.57771125
CGCGGTA17500.017.54857322
ATATACT852.7241667E-517.4117644
GTACTAT752.0678871E-417.2666666
Produced by FastQC (version 0.11.5)