FastQCFastQC Report
Fri 10 Feb 2017
ERR1632661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64053
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3800.5932587076327416No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2810.4386992022231589No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2200.34346556757685043No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1770.2763336611868296No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1750.273211246936131No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1750.273211246936131No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1730.2700888326854324No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1700.26540521130938444No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1670.2607215899333365No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1540.2404258973037953No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1470.22949744742635003No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1450.22637503317565144No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1400.21856899754890483No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1400.21856899754890483No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1400.21856899754890483No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1360.21232416904750753No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1350.2107629619221582No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1290.2013957191700623No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1280.19983451204471295RNA PCR Primer, Index 26 (100% over 22bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1280.19983451204471295No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1280.19983451204471295No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1270.19827330491936365No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1230.1920284764179664No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1200.18734485504191842No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1170.18266123366587045No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1080.16861036953772657No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1070.16704916241237724No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1060.16548795528702792No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1040.1623655410363293No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1020.15924312678563063No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT990.1545595054095827No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA980.15299829828423336No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG980.15299829828423336No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC970.15143709115888407No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA970.15143709115888407No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA940.14675346978283607No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC910.14206984840678813No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC900.1405086412814388No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG900.1405086412814388No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA900.1405086412814388No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG890.13894743415608948No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG880.13738622703074016No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC870.13582501990539084No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA850.13270260565469222No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC840.1311413985293429No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC840.1311413985293429No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC820.12801898427864425No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA810.12645777715329493No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC810.12645777715329493No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA810.12645777715329493No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG790.12333536290259628No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG780.12177415577724697No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT770.12021294865189765No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG770.12021294865189765No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA770.12021294865189765No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA750.117090534401199No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA730.11396812015050037No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT720.11240691302515105No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG720.11240691302515105No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA710.11084570589980172No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT710.11084570589980172No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT700.10928449877445241No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC680.10616208452375377No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG670.10460087739840446No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA660.10303967027305513No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT660.10303967027305513No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC660.10303967027305513No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC650.10147846314770581No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC650.10147846314770581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1450.034.4482771
CTACACA250.005458159429.5999984
CTAAGTC250.005458159429.59999834
AACCCAG451.3042556E-424.66666631
ACCCAGT451.3042556E-424.66666632
GTATCAA2200.022.7045462
GCGGTAA1350.021.92592623
CGCGGTA1400.021.14285922
TCAAGGC450.003787089820.5555553
ACATCTA555.070598E-420.18181830
TTAGCCC656.7730114E-519.92307729
CGGTAAT1500.019.73333424
GGTAATA1500.019.73333425
CCAGCAG1600.019.6562514
CCGCGGT1600.019.6562521
CAGCCGC1600.019.6562518
CTTATAC851.2081964E-619.58823637
GCAGCCG1650.019.06060817
CGTGCCA1650.019.06060810
AGCCGCG1650.019.06060819
Produced by FastQC (version 0.11.5)