##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632661.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64053
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02220036532247	34.0	33.0	34.0	31.0	34.0
2	33.15647979017376	34.0	33.0	34.0	31.0	34.0
3	33.22714002466707	34.0	33.0	34.0	31.0	34.0
4	36.51234134232589	37.0	37.0	37.0	35.0	37.0
5	36.49032832185846	37.0	37.0	37.0	35.0	37.0
6	36.54462710567811	37.0	37.0	37.0	35.0	37.0
7	36.5512778480321	37.0	37.0	37.0	35.0	37.0
8	36.53441681107832	37.0	37.0	37.0	35.0	37.0
9	38.32288885766474	39.0	39.0	39.0	37.0	39.0
10	38.350194370287106	39.0	39.0	39.0	37.0	39.0
11	38.42144786348805	39.0	39.0	39.0	37.0	39.0
12	38.37606357235414	39.0	39.0	39.0	37.0	39.0
13	38.42294662232838	39.0	39.0	39.0	37.0	39.0
14	39.93614662857321	41.0	40.0	41.0	38.0	41.0
15	39.95716047648042	41.0	40.0	41.0	38.0	41.0
16	39.89038764772923	41.0	40.0	41.0	38.0	41.0
17	39.897256959080764	41.0	40.0	41.0	38.0	41.0
18	39.92040966074969	41.0	40.0	41.0	38.0	41.0
19	39.91669398779136	41.0	40.0	41.0	38.0	41.0
20	39.912416280267905	41.0	40.0	41.0	38.0	41.0
21	39.856728022106694	41.0	40.0	41.0	38.0	41.0
22	39.82783007821648	41.0	40.0	41.0	38.0	41.0
23	39.80289760042465	41.0	40.0	41.0	38.0	41.0
24	39.774140165175716	41.0	40.0	41.0	38.0	41.0
25	39.72744446005652	41.0	40.0	41.0	38.0	41.0
26	39.63013441993349	41.0	40.0	41.0	37.0	41.0
27	39.4768551043667	41.0	39.0	41.0	37.0	41.0
28	39.443898021950574	41.0	39.0	41.0	37.0	41.0
29	39.38909965185081	41.0	39.0	41.0	36.0	41.0
30	39.37359686509609	41.0	39.0	41.0	36.0	41.0
31	39.2907123788113	41.0	39.0	41.0	36.0	41.0
32	39.25923844316426	41.0	39.0	41.0	36.0	41.0
33	39.16222503239505	41.0	39.0	41.0	35.0	41.0
34	39.134466769706336	41.0	39.0	41.0	35.0	41.0
35	39.0388740574212	40.0	39.0	41.0	35.0	41.0
36	38.97275693566265	40.0	39.0	41.0	35.0	41.0
37	38.915569918661106	40.0	39.0	41.0	35.0	41.0
38	38.82729926779386	40.0	38.0	41.0	35.0	41.0
39	38.672427520959204	40.0	38.0	41.0	35.0	41.0
40	38.6129455294834	40.0	38.0	41.0	35.0	41.0
41	38.55383822771767	40.0	38.0	41.0	35.0	41.0
42	38.474029319469814	40.0	38.0	41.0	35.0	41.0
43	37.704198085960066	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	2.0
21	2.0
22	14.0
23	19.0
24	35.0
25	64.0
26	84.0
27	110.0
28	167.0
29	217.0
30	302.0
31	376.0
32	513.0
33	673.0
34	964.0
35	1592.0
36	2546.0
37	5095.0
38	13858.0
39	37418.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.26729427193106	15.596459182239709	12.931478619268418	28.204767926560812
2	22.98877492076874	19.619689944264906	30.71362777699718	26.677907357969183
3	22.406444663013442	18.74073033269324	28.790220598566812	30.062604405726507
4	15.42941001982733	16.31773687415109	35.63923625747428	32.613616848547295
5	17.674425866079652	32.135887468190404	32.13276505393971	18.056921611790237
6	31.100807144083802	34.977284436326165	16.266217039014567	17.655691380575462
7	28.646589543034672	27.15875915257677	19.071706243267293	25.12294506112126
8	26.784069442492935	28.98537148923548	20.888951337173904	23.341607731097685
9	27.948729958003526	12.205517305980983	17.120197336580645	42.725555399434846
10	20.83274788066133	21.53216867281782	26.64980562971289	30.985277816807955
11	40.61636457308791	19.251245062682464	17.70096638721059	22.43142397701903
12	22.279986885860147	23.05746803428411	26.57330648057078	28.089238599284965
13	38.215228014300656	16.96251541692036	18.96242174449284	25.85983482428614
14	24.326729427193104	20.23012193027649	22.398638627386696	33.04451001514371
15	31.272539927872227	22.798307651476122	18.885922595350728	27.043229825300923
16	25.049568326229842	23.433718951493297	22.682778324200275	28.833934398076593
17	27.87847563736281	22.948183535509656	20.479915070332382	28.693425756795154
18	27.27897210122867	19.316815761947137	22.49855588340905	30.905656253415142
19	29.397530170327695	21.755421291742774	22.60159555368211	26.245452984247418
20	31.814278800368445	19.252806269807817	21.10595912759746	27.826955802226284
21	28.331225703714114	20.472109034705635	21.2402229403775	29.956442321202754
22	28.99005511061152	22.17694721558709	20.083368460493652	28.74962921330773
23	29.82530092267341	20.473670241830984	21.012286700076498	28.688742135419105
24	28.609120572026292	19.833575320437763	22.239395500601063	29.317908606934886
25	30.001717327837884	21.324528125146365	21.533729879943174	27.14002466707258
26	29.370989649196762	21.961500632288885	21.15435654848329	27.513153170031067
27	27.767629931463006	21.112203956098856	24.38761650508173	26.73254960735641
28	27.00576085429254	23.140212011927623	20.245734001529982	29.60829313224986
29	27.80666010959674	23.937988852981125	20.751565110143165	27.503785927278972
30	27.778558381340453	20.80776856665574	23.95203971710927	27.461633334894543
31	29.46934569809377	21.825675612383495	21.232416904750753	27.472561784771987
32	26.100260721589937	20.681310789502444	22.61564641781025	30.602782071097373
33	26.4031349039077	20.990429800321607	24.0004371379951	28.60599815777559
34	26.890231527016688	21.31203846814357	23.340046523972337	28.457683480867406
35	25.93008914492686	23.416545673114452	23.85680608246296	26.79655909949573
36	27.043229825300923	20.828064259285277	25.00585452672006	27.122851388693736
37	27.92375064399794	20.54860818384775	23.88178539646855	27.645855775685764
38	26.053424507829455	19.88197274132359	25.90042620954522	28.16417654130173
39	25.316534744664576	18.570558756030163	27.107239317440246	29.005667181865018
40	25.110455404118465	19.758637378420996	28.515448144505335	26.61545907295521
41	22.86856197211684	19.07951227889404	29.122757716266218	28.929168032722902
42	21.112203956098856	20.16142881676112	30.92439073891933	27.801976488220692
43	20.006869311351537	21.269885875759137	29.60829313224986	29.11495168063947
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	4.0
9	5.0
10	7.0
11	9.0
12	9.0
13	21.0
14	33.0
15	52.5
16	72.0
17	64.5
18	57.0
19	57.0
20	69.0
21	81.0
22	63.5
23	46.0
24	38.0
25	30.0
26	30.0
27	34.5
28	39.0
29	42.0
30	45.0
31	54.5
32	64.0
33	64.0
34	116.5
35	169.0
36	160.0
37	151.0
38	307.0
39	463.0
40	463.0
41	782.0
42	1101.0
43	1492.5
44	1884.0
45	3244.5
46	4605.0
47	4605.0
48	5490.5
49	6376.0
50	7249.5
51	8123.0
52	8317.5
53	8512.0
54	8512.0
55	7877.5
56	7243.0
57	7026.0
58	6809.0
59	6285.5
60	5762.0
61	5762.0
62	5260.0
63	4758.0
64	3724.0
65	2690.0
66	2230.0
67	1770.0
68	1770.0
69	1555.5
70	1341.0
71	1104.0
72	867.0
73	653.0
74	439.0
75	439.0
76	349.5
77	260.0
78	183.5
79	107.0
80	99.0
81	91.0
82	91.0
83	60.0
84	29.0
85	19.5
86	10.0
87	7.0
88	4.0
89	4.0
90	3.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	64053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.30384213073548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37496206884926	37.67973397030584
2	7.737954752351732	7.16594070535338
3	2.9535722714858896	4.102852325418013
4	1.733032131899255	3.209841849718202
5	1.1193904042617755	2.5916038280798714
6	0.8429144610404936	2.34181068802398
7	0.5799251491958596	1.8796933789205814
8	0.4754037560268384	1.761041637394033
9	0.3405374422603594	1.4191372769425319
>10	2.501770120368185	22.993458542144786
>50	0.23938770693550018	7.701434749348196
>100	0.10114973532485924	7.153451048350584
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	380	0.5932587076327416	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	281	0.4386992022231589	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	220	0.34346556757685043	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	177	0.2763336611868296	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	175	0.273211246936131	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	175	0.273211246936131	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	173	0.2700888326854324	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	170	0.26540521130938444	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	167	0.2607215899333365	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	154	0.2404258973037953	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	147	0.22949744742635003	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	145	0.22637503317565144	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	140	0.21856899754890483	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	140	0.21856899754890483	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	140	0.21856899754890483	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	136	0.21232416904750753	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	135	0.2107629619221582	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	129	0.2013957191700623	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	128	0.19983451204471295	RNA PCR Primer, Index 26 (100% over 22bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	128	0.19983451204471295	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	128	0.19983451204471295	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	127	0.19827330491936365	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	123	0.1920284764179664	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	120	0.18734485504191842	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	117	0.18266123366587045	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	108	0.16861036953772657	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	107	0.16704916241237724	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	106	0.16548795528702792	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	104	0.1623655410363293	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	102	0.15924312678563063	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	99	0.1545595054095827	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	98	0.15299829828423336	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	98	0.15299829828423336	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	97	0.15143709115888407	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	97	0.15143709115888407	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	94	0.14675346978283607	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	91	0.14206984840678813	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	90	0.1405086412814388	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	90	0.1405086412814388	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	90	0.1405086412814388	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	89	0.13894743415608948	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	88	0.13738622703074016	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	87	0.13582501990539084	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	85	0.13270260565469222	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	84	0.1311413985293429	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	84	0.1311413985293429	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	82	0.12801898427864425	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	81	0.12645777715329493	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	81	0.12645777715329493	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	81	0.12645777715329493	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	79	0.12333536290259628	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	78	0.12177415577724697	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	77	0.12021294865189765	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	77	0.12021294865189765	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	77	0.12021294865189765	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	75	0.117090534401199	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	73	0.11396812015050037	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	72	0.11240691302515105	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	72	0.11240691302515105	No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA	71	0.11084570589980172	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	71	0.11084570589980172	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	70	0.10928449877445241	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	68	0.10616208452375377	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	67	0.10460087739840446	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	66	0.10303967027305513	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	66	0.10303967027305513	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	66	0.10303967027305513	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	65	0.10147846314770581	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	65	0.10147846314770581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0015612071253493201	0.0
12	0.0	0.0	0.0	0.0015612071253493201	0.0
13	0.0	0.0	0.0	0.0015612071253493201	0.0
14	0.0	0.0	0.0	0.0015612071253493201	0.0
15	0.0	0.0	0.0	0.0015612071253493201	0.0
16	0.0	0.0	0.0	0.0015612071253493201	0.0
17	0.0	0.0	0.0	0.0031224142506986402	0.0
18	0.0015612071253493201	0.0	0.0	0.0031224142506986402	0.0
19	0.0015612071253493201	0.0	0.0	0.0031224142506986402	0.0
20	0.0015612071253493201	0.0	0.0	0.00468362137604796	0.0
21	0.0015612071253493201	0.0	0.0	0.0062448285013972805	0.0
22	0.0015612071253493201	0.0	0.0	0.010928449877445241	0.0
23	0.0015612071253493201	0.0	0.0	0.012489657002794561	0.0
24	0.0015612071253493201	0.0	0.0	0.012489657002794561	0.0
25	0.0015612071253493201	0.0	0.0	0.01717327837884252	0.0
26	0.0015612071253493201	0.0	0.0	0.02654052113093844	0.0
27	0.0015612071253493201	0.0	0.0	0.07337673489141805	0.0
28	0.0015612071253493201	0.0	0.0	0.24823193293054188	0.0
29	0.0015612071253493201	0.0	0.0	0.46992334473014535	0.0
30	0.0015612071253493201	0.0	0.0	0.7618690771704683	0.0
31	0.0015612071253493201	0.0	0.0	1.5377890184690803	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	145	0.0	34.448277	1
CTACACA	25	0.0054581594	29.599998	4
CTAAGTC	25	0.0054581594	29.599998	34
AACCCAG	45	1.3042556E-4	24.666666	31
ACCCAGT	45	1.3042556E-4	24.666666	32
GTATCAA	220	0.0	22.704546	2
GCGGTAA	135	0.0	21.925926	23
CGCGGTA	140	0.0	21.142859	22
TCAAGGC	45	0.0037870898	20.555555	3
ACATCTA	55	5.070598E-4	20.181818	30
TTAGCCC	65	6.7730114E-5	19.923077	29
CGGTAAT	150	0.0	19.733334	24
GGTAATA	150	0.0	19.733334	25
CCAGCAG	160	0.0	19.65625	14
CCGCGGT	160	0.0	19.65625	21
CAGCCGC	160	0.0	19.65625	18
CTTATAC	85	1.2081964E-6	19.588236	37
GCAGCCG	165	0.0	19.060608	17
CGTGCCA	165	0.0	19.060608	10
AGCCGCG	165	0.0	19.060608	19
>>END_MODULE