FastQCFastQC Report
Fri 10 Feb 2017
ERR1632660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25637
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA780.3042477668994032No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA660.25744041814564883No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA580.2262355189764793No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT570.2223349065803331TruSeq Adapter, Index 12 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT470.18332878261887117No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA430.16772633303428638No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG370.14432265865740923No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC360.140422046261263No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT360.140422046261263No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT340.13262082146897064No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA330.12872020907282442No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT320.12481959667667825No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC320.12481959667667825No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG320.12481959667667825No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA320.12481959667667825No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT310.12091898428053204No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC310.12091898428053204No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC300.11701837188438585No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC300.11701837188438585No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC290.11311775948823966No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC290.11311775948823966No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA290.11311775948823966No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA280.10921714709209344No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG270.10531653469594726No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT260.10141592229980106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA250.00539778529.623
GCAGCCG250.00539778529.617
CGCGGTA250.00539778529.622
CGTGCCA250.00539778529.610
CCGCGGT250.00539778529.621
GTGCCAG250.00539778529.611
TGCCAGC500.006853236418.512
TCTTATA608.905062E-418.537
GTCTCTT1701.2423698E-915.23529437
CTCTTAT750.00396053714.836
TGTCTCT2401.5541991E-710.79166736
CTGTCTC2851.6411504E-69.08771935
Produced by FastQC (version 0.11.5)