##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25637
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93985255685143	34.0	33.0	34.0	31.0	34.0
2	33.08682763193821	34.0	33.0	34.0	31.0	34.0
3	33.167453290166556	34.0	33.0	34.0	31.0	34.0
4	36.47852712875922	37.0	37.0	37.0	35.0	37.0
5	36.448882474548505	37.0	37.0	37.0	35.0	37.0
6	36.48355891875024	37.0	37.0	37.0	35.0	37.0
7	36.49331044974061	37.0	37.0	37.0	35.0	37.0
8	36.471662050942	37.0	37.0	37.0	35.0	37.0
9	38.25240862815462	39.0	39.0	39.0	37.0	39.0
10	38.25147248117955	39.0	39.0	39.0	37.0	39.0
11	38.338105082497954	39.0	39.0	39.0	37.0	39.0
12	38.28259936810079	39.0	39.0	39.0	37.0	39.0
13	38.326286226937626	39.0	39.0	39.0	37.0	39.0
14	39.84358544291454	41.0	40.0	41.0	38.0	41.0
15	39.834887077271134	41.0	40.0	41.0	38.0	41.0
16	39.79673908803682	41.0	40.0	41.0	38.0	41.0
17	39.796622069664934	41.0	40.0	41.0	38.0	41.0
18	39.80867496196903	41.0	40.0	41.0	38.0	41.0
19	39.83824160393182	41.0	40.0	41.0	38.0	41.0
20	39.780512540468855	41.0	40.0	41.0	38.0	41.0
21	39.73717673674767	41.0	40.0	41.0	38.0	41.0
22	39.69883371689355	41.0	40.0	41.0	37.0	41.0
23	39.687872996060385	41.0	40.0	41.0	38.0	41.0
24	39.668681983071345	41.0	40.0	41.0	37.0	41.0
25	39.620236377111205	41.0	40.0	41.0	37.0	41.0
26	39.5165971057456	41.0	40.0	41.0	37.0	41.0
27	39.43246089636073	41.0	39.0	41.0	37.0	41.0
28	39.35643796075984	41.0	39.0	41.0	36.0	41.0
29	39.320006240979836	41.0	39.0	41.0	36.0	41.0
30	39.311307875336425	41.0	39.0	41.0	36.0	41.0
31	39.22740570269532	41.0	39.0	41.0	36.0	41.0
32	39.168272418769746	41.0	39.0	41.0	35.0	41.0
33	39.08624254007879	40.0	39.0	41.0	35.0	41.0
34	39.0746967273862	40.0	39.0	41.0	35.0	41.0
35	38.99765963256231	40.0	39.0	41.0	35.0	41.0
36	38.92362600928346	40.0	39.0	41.0	35.0	41.0
37	38.87404922572844	40.0	38.0	41.0	35.0	41.0
38	38.75636774973671	40.0	38.0	41.0	35.0	41.0
39	38.66287007060109	40.0	38.0	41.0	35.0	41.0
40	38.59117681475992	40.0	38.0	41.0	35.0	41.0
41	38.526231618364086	40.0	38.0	41.0	35.0	41.0
42	38.48320786363459	40.0	38.0	41.0	35.0	41.0
43	37.711939774544604	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	0.0
22	4.0
23	12.0
24	22.0
25	21.0
26	34.0
27	48.0
28	54.0
29	92.0
30	113.0
31	190.0
32	240.0
33	309.0
34	388.0
35	693.0
36	1137.0
37	2182.0
38	5758.0
39	14338.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.38651168233413	16.191442056402856	12.286929047860514	28.135117213402506
2	22.299801068767795	19.179311151850843	30.79533486757421	27.725552911807156
3	21.95654717790693	19.093497679135623	28.423762530717323	30.526192612240123
4	16.304559815891096	15.391816515192886	33.038186995358274	35.26543667355775
5	17.51765027109256	32.57401412021687	32.61302024417834	17.295315364512227
6	35.573585052853296	33.127901080469634	14.49467566407926	16.803838202597806
7	30.514490775051684	27.175566563950543	19.37434177165815	22.93560088933963
8	27.526621679603696	30.849943441120253	19.577173616257753	22.046261263018295
9	27.70214923743028	12.294730272652806	17.033974333970434	42.96914615594648
10	19.955533018683933	22.752272106720756	29.180481335569684	28.11171353902563
11	40.50395912158209	18.851659710574562	18.098841518118345	22.545539649725008
12	23.286656004992786	22.904395990170457	25.810352225299372	27.99859577953739
13	35.88563404454499	16.831142489370833	19.9360299567032	27.347193509380972
14	25.303272613800367	18.496703982525258	22.089167999375903	34.110855404298476
15	30.143932597417795	23.450481725630922	18.98818114443968	27.417404532511604
16	26.945430432577915	23.220345594258298	21.5235792019347	28.310644771229082
17	28.23263252330616	22.728868432343877	20.505519366540547	28.53297967780942
18	26.945430432577915	21.125716737527792	22.845886804228265	29.08296602566603
19	29.71876584623786	21.929242891133907	22.08526738697976	26.26672387564848
20	29.855287280102978	20.404103444240747	21.983851464679955	27.756757810976325
21	28.915239692631744	21.49627491516168	21.008698365643404	28.57978702656317
22	28.985450715762372	21.781019620080354	20.466513242579083	28.76701642157819
23	29.24679174630417	21.574287163084605	21.266138783789053	27.91278230682217
24	29.293599095057925	21.110114287943208	21.504076139953973	28.092210477044897
25	29.882591566876	21.394858992861877	21.500175527557825	27.222373912704295
26	29.882591566876	21.827826968834106	21.242735109412177	27.04684635487772
27	28.146819050590942	21.379256543277293	23.13453212154308	27.339392284588683
28	26.785505324335922	22.213987596052583	21.757615945703478	29.24289113390802
29	27.674844950657253	23.181339470296834	21.234933884619885	27.908881694426025
30	27.690447400241837	21.5235792019347	22.923899052151185	27.862074345672273
31	29.515934001638257	21.43776572921949	21.078909388774036	27.96739088036822
32	26.71529430120529	21.61329328704607	22.381713929086867	29.28969848266178
33	27.1638647267621	21.355852868900417	22.838085579435973	28.64219682490151
34	27.105355540819907	21.656200023403674	22.78737761828607	28.451066817490343
35	26.9610328821625	22.76787455630534	23.376370090104146	26.89472247142801
36	27.12095799040449	21.266138783789053	24.211101142879432	27.40180208292702
37	27.990794554745097	20.220774661621874	23.44268050083863	28.345750282794395
38	25.993681007918244	20.31828997152553	25.79084916331864	27.897179857237585
39	26.040488356671997	19.545968717088584	26.118500604594924	28.295042321644498
40	25.197956079104422	20.201271599641142	27.46421188126536	27.136560439989076
41	23.551897647930726	19.588875453446192	28.11171353902563	28.747513359597455
42	22.28809923157936	20.5523267152943	29.722666458634006	27.43690759449234
43	21.273940008581345	20.73955611030932	29.371611342980845	28.614892538128483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	1.5
9	0.0
10	2.0
11	4.0
12	4.0
13	7.0
14	10.0
15	12.0
16	14.0
17	16.0
18	18.0
19	18.0
20	19.0
21	20.0
22	18.5
23	17.0
24	26.0
25	35.0
26	35.0
27	41.0
28	47.0
29	64.5
30	82.0
31	109.5
32	137.0
33	137.0
34	162.0
35	187.0
36	245.5
37	304.0
38	386.5
39	469.0
40	469.0
41	579.0
42	689.0
43	788.0
44	887.0
45	1225.5
46	1564.0
47	1564.0
48	1823.0
49	2082.0
50	2356.0
51	2630.0
52	2828.0
53	3026.0
54	3026.0
55	2890.0
56	2754.0
57	2605.5
58	2457.0
59	2338.5
60	2220.0
61	2220.0
62	2084.0
63	1948.0
64	1613.5
65	1279.0
66	1095.5
67	912.0
68	912.0
69	802.0
70	692.0
71	584.0
72	476.0
73	378.0
74	280.0
75	280.0
76	222.0
77	164.0
78	136.5
79	109.0
80	84.5
81	60.0
82	60.0
83	47.0
84	34.0
85	23.5
86	13.0
87	10.0
88	7.0
89	7.0
90	5.5
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	25637.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.17158793930648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.18700357161896	64.5278308694465
2	6.023775254544486	8.8153840152904
3	2.126979050055973	4.6690330381869956
4	1.1141318833626526	3.2609119631782186
5	0.6823391438776054	2.496391933533565
6	0.44778506316967853	1.9659086476576821
7	0.23988485526947068	1.2286929047860515
8	0.24521562983101444	1.4354253617817998
9	0.09595394210778826	0.6318992081756836
>10	0.8156085079162002	9.958263447361237
>50	0.02132309824617517	1.0102586106018645
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	78	0.3042477668994032	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	66	0.25744041814564883	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	58	0.2262355189764793	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	57	0.2223349065803331	TruSeq Adapter, Index 12 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	47	0.18332878261887117	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	43	0.16772633303428638	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	37	0.14432265865740923	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	36	0.140422046261263	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	36	0.140422046261263	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	34	0.13262082146897064	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	33	0.12872020907282442	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	32	0.12481959667667825	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	32	0.12481959667667825	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	32	0.12481959667667825	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	32	0.12481959667667825	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	31	0.12091898428053204	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	31	0.12091898428053204	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	30	0.11701837188438585	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	30	0.11701837188438585	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	29	0.11311775948823966	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	29	0.11311775948823966	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	29	0.11311775948823966	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	28	0.10921714709209344	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	27	0.10531653469594726	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	26	0.10141592229980106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0039006123961461948	0.0
25	0.0	0.0	0.0	0.011701837188438586	0.0
26	0.0	0.0	0.0	0.011701837188438586	0.0
27	0.0	0.0	0.0	0.06631041073448532	0.0
28	0.0	0.0	0.0	0.19893123220345593	0.0
29	0.0	0.0	0.0	0.33155205367242657	0.0
30	0.0	0.0	0.0	0.49537777431056673	0.0
31	0.0	0.0	0.0	1.002457385809572	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTAA	25	0.005397785	29.6	23
GCAGCCG	25	0.005397785	29.6	17
CGCGGTA	25	0.005397785	29.6	22
CGTGCCA	25	0.005397785	29.6	10
CCGCGGT	25	0.005397785	29.6	21
GTGCCAG	25	0.005397785	29.6	11
TGCCAGC	50	0.0068532364	18.5	12
TCTTATA	60	8.905062E-4	18.5	37
GTCTCTT	170	1.2423698E-9	15.235294	37
CTCTTAT	75	0.003960537	14.8	36
TGTCTCT	240	1.5541991E-7	10.791667	36
CTGTCTC	285	1.6411504E-6	9.087719	35
>>END_MODULE