FastQCFastQC Report
Fri 10 Feb 2017
ERR1632659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8520
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT650.7629107981220657RNA PCR Primer, Index 17 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA570.6690140845070423No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA480.5633802816901409No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA350.41079812206572774No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC340.3990610328638498No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA320.3755868544600939No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC310.363849765258216No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT280.32863849765258213No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA280.32863849765258213No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC260.3051643192488263No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA250.2934272300469483No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT230.2699530516431925No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT220.25821596244131456No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA220.25821596244131456No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA210.24647887323943662No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC210.24647887323943662No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC190.22300469483568075No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT190.22300469483568075No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG190.22300469483568075No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC190.22300469483568075No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG180.21126760563380279No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC180.21126760563380279No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC170.1995305164319249No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA170.1995305164319249No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT170.1995305164319249No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC160.18779342723004694No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC160.18779342723004694RNA PCR Primer, Index 17 (95% over 22bp)
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA160.18779342723004694No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT160.18779342723004694No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA150.17605633802816903No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC150.17605633802816903No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC140.16431924882629106No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT130.15258215962441316No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA130.15258215962441316No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA130.15258215962441316No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC130.15258215962441316No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA130.15258215962441316No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT130.15258215962441316No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC120.14084507042253522No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG120.14084507042253522No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG120.14084507042253522No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC120.14084507042253522No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA120.14084507042253522No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG120.14084507042253522No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC120.14084507042253522No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC120.14084507042253522No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC120.14084507042253522No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT120.14084507042253522No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG120.14084507042253522No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA110.12910798122065728No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA110.12910798122065728No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC110.12910798122065728No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC110.12910798122065728No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC110.12910798122065728No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC110.12910798122065728No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA110.12910798122065728No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA110.12910798122065728No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG100.11737089201877934No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT100.11737089201877934No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT100.11737089201877934No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC100.11737089201877934No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC100.11737089201877934No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT100.11737089201877934No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC100.11737089201877934No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT100.11737089201877934No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA100.11737089201877934No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA100.11737089201877934No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAG100.11737089201877934No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA100.11737089201877934No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA100.11737089201877934No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG100.11737089201877934No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT100.11737089201877934No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC100.11737089201877934No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG100.11737089201877934No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG90.10563380281690139No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG90.10563380281690139No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG90.10563380281690139No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT90.10563380281690139No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT90.10563380281690139No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG90.10563380281690139No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG90.10563380281690139No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT90.10563380281690139No Hit
TCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGC90.10563380281690139No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT90.10563380281690139No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG90.10563380281690139No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG90.10563380281690139No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT90.10563380281690139No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA90.10563380281690139No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG90.10563380281690139No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG90.10563380281690139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAT250.005198702729.59999830
AACGTCA250.005198702729.59999828
ACGTCAA250.005198702729.59999829
GTCAATG250.005198702729.59999831
TAACGTC250.005198702729.59999827
TTCTGCG250.005198702729.59999818
TCTGCGG250.005198702729.59999819
CTGCGGG250.005198702729.59999820
TTCTTCT250.005198702729.59999815
TCTTCTG250.005198702729.59999816
GCTTCTT250.005198702729.59999813
GTAACGT250.005198702729.59999826
TGCGGGT250.005198702729.59999821
CTTCTGC250.005198702729.59999817
Produced by FastQC (version 0.11.5)