FastQCFastQC Report
Fri 10 Feb 2017
ERR1632657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76491
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1790.23401445921742425No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1350.17649135192375573No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1320.17256932188100563No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1180.15426651501483835No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1110.14511511158175472No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1080.1411930815390046No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1060.13857839484383785No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1010.13204167810592096No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA970.12681230471558746No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT930.12158293132525394TruSeq Adapter, Index 11 (95% over 21bp)
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC890.11635355793492046No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA860.11243152789217033No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT850.11112418454458693No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA810.10589481115425345No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC800.10458746780667007No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA770.10066543776391995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC200.001831210837.037
ATACGGC251.2228782E-436.99999629
TACGGCT303.5684142E-430.83333230
GGTATCA850.030.4705891
TTAGCCC405.8690093E-527.7529
ACCATGC358.79357E-426.4285728
GCGGTAA851.904482E-923.94117723
TTATACA551.8727214E-523.54545437
CCGGTGC400.001914834423.1258
GTATCAA1200.023.1249982
AGCCGCG903.6889105E-922.61111319
CGGTAAT903.6889105E-922.61111324
CGTGCCA855.0231392E-821.76470610
GTAATAC956.884875E-921.42105326
GCCAGCA956.884875E-921.42105313
TCTTATA2150.020.65116337
TAATACG909.1913535E-820.55555727
CAGCCGC1001.2430974E-820.34999818
GGTAATA1101.6807462E-920.18181825
TACCACG759.081705E-619.7333324
Produced by FastQC (version 0.11.5)