##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632657.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76491
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97929168137428	34.0	33.0	34.0	31.0	34.0
2	33.12235426390033	34.0	33.0	34.0	31.0	34.0
3	33.191839562824384	34.0	33.0	34.0	31.0	34.0
4	36.50626871135166	37.0	37.0	37.0	35.0	37.0
5	36.46720529212587	37.0	37.0	37.0	35.0	37.0
6	36.50745839379796	37.0	37.0	37.0	35.0	37.0
7	36.50811206547176	37.0	37.0	37.0	35.0	37.0
8	36.47610830032291	37.0	37.0	37.0	35.0	37.0
9	38.256160855525486	39.0	39.0	39.0	37.0	39.0
10	38.281993960073734	39.0	39.0	39.0	37.0	39.0
11	38.36521943758089	39.0	39.0	39.0	37.0	39.0
12	38.31548809663882	39.0	39.0	39.0	37.0	39.0
13	38.35248591337543	39.0	39.0	39.0	37.0	39.0
14	39.878756977945116	41.0	40.0	41.0	38.0	41.0
15	39.87552783987659	41.0	40.0	41.0	38.0	41.0
16	39.825613470865854	41.0	40.0	41.0	38.0	41.0
17	39.82418846661699	41.0	40.0	41.0	38.0	41.0
18	39.839471310350234	41.0	40.0	41.0	38.0	41.0
19	39.83822933417003	41.0	40.0	41.0	38.0	41.0
20	39.806186348720765	41.0	40.0	41.0	38.0	41.0
21	39.76014171601888	41.0	40.0	41.0	38.0	41.0
22	39.730974885934295	41.0	40.0	41.0	38.0	41.0
23	39.6985658443477	41.0	40.0	41.0	38.0	41.0
24	39.69151926370423	41.0	40.0	41.0	38.0	41.0
25	39.64529160293368	41.0	40.0	41.0	37.0	41.0
26	39.54466538547019	41.0	40.0	41.0	37.0	41.0
27	39.45917820397171	41.0	39.0	41.0	37.0	41.0
28	39.41718633564733	41.0	39.0	41.0	37.0	41.0
29	39.37903805676485	41.0	39.0	41.0	37.0	41.0
30	39.31880874874168	41.0	39.0	41.0	36.0	41.0
31	39.25056542599783	41.0	39.0	41.0	36.0	41.0
32	39.21120131780209	41.0	39.0	41.0	36.0	41.0
33	39.135453844243116	40.0	39.0	41.0	35.0	41.0
34	39.106430821926764	40.0	39.0	41.0	35.0	41.0
35	39.02795100077133	40.0	39.0	41.0	35.0	41.0
36	38.98398504399211	40.0	39.0	41.0	35.0	41.0
37	38.929978690303436	40.0	39.0	41.0	35.0	41.0
38	38.888901962322365	40.0	39.0	41.0	35.0	41.0
39	38.78824959799192	40.0	38.0	41.0	35.0	41.0
40	38.701350485678056	40.0	38.0	41.0	35.0	41.0
41	38.64487325306245	40.0	38.0	41.0	35.0	41.0
42	38.5703546822502	40.0	38.0	41.0	35.0	41.0
43	37.83215018760377	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	3.0
20	2.0
21	5.0
22	12.0
23	28.0
24	31.0
25	89.0
26	99.0
27	139.0
28	175.0
29	268.0
30	352.0
31	478.0
32	635.0
33	807.0
34	1254.0
35	1963.0
36	3203.0
37	6217.0
38	16978.0
39	43751.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.11487625995215	16.333947784706698	12.455060072426821	28.09611588291433
2	21.946372775882132	19.102900994888287	31.427226732556772	27.52349949667281
3	21.254788145010526	19.442810265259965	28.639970715509016	30.662430874220497
4	15.92867134695585	15.509014132381587	33.52420546208051	35.03810905858206
5	17.441267600109818	32.648285419199645	32.8326208312089	17.07782614948164
6	35.112627629394304	33.47321907152476	14.703690630270227	16.71046266881071
7	30.764403655332	27.200585689819718	19.42842948843655	22.606581166411736
8	27.715678968767566	30.453255938607153	19.71996705494764	22.111098037677635
9	27.96930357819874	12.723065458681413	17.05952334261547	42.24810762050437
10	19.56047116654247	22.96610058699716	29.24396334209253	28.229464904367834
11	40.311932122733396	18.951249166568616	18.260971879044593	22.4758468316534
12	23.201422389562172	23.470735119164345	26.442326548221363	26.885515943052123
13	35.332261311788315	17.34975356577898	20.24290439398099	27.075080728451713
14	24.286517368056373	19.380057784575964	22.515067132080897	33.818357715286766
15	29.86625877554222	23.622386947484017	19.628453020616803	26.882901256356956
16	25.975604973134093	23.423670758651344	22.37256670719431	28.22815756102025
17	27.279026290674718	23.291629080545423	21.321462655737275	28.10788197304258
18	26.977029977382962	21.0835261664771	22.894196702880077	29.04524715325986
19	28.8138473807376	21.924147938973213	22.503301041952646	26.758703638336534
20	29.927703912878638	20.887424664339598	22.25229111921664	26.932580303565125
21	27.83333987005007	21.90323044541188	21.707128943274373	28.55630074126368
22	28.63081931207593	22.815756102025077	20.59065772443817	27.962766861460825
23	28.60467244512426	22.060111647121882	21.730661123530872	27.60455478422298
24	28.11180400308533	21.49664666431345	22.073185080597717	28.3183642520035
25	28.539305277745093	21.941143402491797	21.97382698618138	27.545724333581727
26	28.391575479468173	22.206534102051222	22.423553097750062	26.978337320730546
27	27.399301878652388	22.01566197330405	23.63415303761227	26.950883110431295
28	26.766547698422038	22.93080231661241	21.874468891765044	28.428181093200504
29	27.502582003111474	23.172660835915337	22.049652900341215	27.27510426063197
30	27.088154161927548	21.942450745839377	23.574015243623432	27.39537984860964
31	27.952308114680157	21.971212299486215	22.671948333790905	27.404531252042723
32	26.2187708357846	21.73327581022604	22.671948333790905	29.376005020198452
33	25.831797204899924	21.773803454001126	24.499614333712465	27.894785007386492
34	26.56129479285145	21.463963080623863	24.215920827286872	27.75882129923782
35	25.874939535370174	22.567360865984234	24.784615183485638	26.773084415159953
36	26.247532389431438	21.0129296257076	25.301015805781073	27.438522179079893
37	26.073655724202847	20.406322312428912	25.34415813625132	28.175863827116913
38	25.286635028957654	19.67682472447739	26.943039050345792	28.09350119621916
39	24.385875462472708	18.779987188035193	28.49485560392726	28.339281745564836
40	24.085186492528532	19.55524179315214	29.53027153521329	26.829300179106035
41	22.409172320926647	19.472879162254383	30.05451621759423	28.063432299224743
42	20.771071106404676	19.899073093566564	31.3383273849211	27.99152841510766
43	19.841549986272895	20.384097475519997	30.84545894288217	28.92889359532494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	3.5
9	3.0
10	7.5
11	12.0
12	12.0
13	19.0
14	26.0
15	44.5
16	63.0
17	67.0
18	71.0
19	71.0
20	83.0
21	95.0
22	92.5
23	90.0
24	92.5
25	95.0
26	95.0
27	123.5
28	152.0
29	236.5
30	321.0
31	387.0
32	453.0
33	453.0
34	598.0
35	743.0
36	855.5
37	968.0
38	1328.0
39	1688.0
40	1688.0
41	2017.5
42	2347.0
43	2848.0
44	3349.0
45	4290.0
46	5231.0
47	5231.0
48	6041.5
49	6852.0
50	7561.0
51	8270.0
52	8450.0
53	8630.0
54	8630.0
55	8279.0
56	7928.0
57	7529.5
58	7131.0
59	6712.0
60	6293.0
61	6293.0
62	5793.0
63	5293.0
64	4383.0
65	3473.0
66	3031.0
67	2589.0
68	2589.0
69	2140.5
70	1692.0
71	1396.5
72	1101.0
73	864.0
74	627.0
75	627.0
76	506.0
77	385.0
78	299.5
79	214.0
80	185.0
81	156.0
82	156.0
83	114.5
84	73.0
85	54.5
86	36.0
87	29.0
88	22.0
89	22.0
90	14.5
91	7.0
92	6.0
93	5.0
94	4.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	76491.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.51275313435568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03161497808955	61.888326731249435
2	5.548137142427263	7.713325750741918
3	1.7039363562844407	3.553359218731615
4	0.9854996144514867	2.7401916565347557
5	0.6074739989844088	2.111359506347152
6	0.42692445129864026	1.7806016394085578
7	0.2764664948938331	1.3452563046632937
8	0.21252186342179008	1.1818383862153718
9	0.15986157868010759	1.0001176609012825
>10	0.9742152677211261	12.617170647527159
>50	0.058302458106862756	2.7741825835719234
>100	0.015045795640480713	1.294269914107542
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	179	0.23401445921742425	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	135	0.17649135192375573	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	132	0.17256932188100563	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	118	0.15426651501483835	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	111	0.14511511158175472	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	108	0.1411930815390046	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	106	0.13857839484383785	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	101	0.13204167810592096	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	97	0.12681230471558746	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	93	0.12158293132525394	TruSeq Adapter, Index 11 (95% over 21bp)
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	89	0.11635355793492046	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	86	0.11243152789217033	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	85	0.11112418454458693	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	81	0.10589481115425345	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	80	0.10458746780667007	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	77	0.10066543776391995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0013073433475833758	0.0
19	0.0	0.0	0.0	0.0026146866951667516	0.0
20	0.0	0.0	0.0	0.0026146866951667516	0.0
21	0.0	0.0	0.0	0.005229373390333503	0.0
22	0.0	0.0	0.0	0.007844060085500254	0.0
23	0.0	0.0	0.0	0.010458746780667006	0.0
24	0.0	0.0	0.0	0.011766090128250382	0.0
25	0.0	0.0	0.0	0.014380776823417134	0.0
26	0.0	0.0	0.0	0.016995463518583886	0.0
27	0.0	0.0	0.0	0.06798185407433555	0.0
28	0.0	0.0	0.0	0.23009242917467415	0.0
29	0.0	0.0	0.0	0.4575701716541815	0.0
30	0.0	0.0	0.0	0.7726399184217752	0.0
31	0.0	0.0	0.0	1.5322064033677165	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	20	0.0018312108	37.0	37
ATACGGC	25	1.2228782E-4	36.999996	29
TACGGCT	30	3.5684142E-4	30.833332	30
GGTATCA	85	0.0	30.470589	1
TTAGCCC	40	5.8690093E-5	27.75	29
ACCATGC	35	8.79357E-4	26.428572	8
GCGGTAA	85	1.904482E-9	23.941177	23
TTATACA	55	1.8727214E-5	23.545454	37
CCGGTGC	40	0.0019148344	23.125	8
GTATCAA	120	0.0	23.124998	2
AGCCGCG	90	3.6889105E-9	22.611113	19
CGGTAAT	90	3.6889105E-9	22.611113	24
CGTGCCA	85	5.0231392E-8	21.764706	10
GTAATAC	95	6.884875E-9	21.421053	26
GCCAGCA	95	6.884875E-9	21.421053	13
TCTTATA	215	0.0	20.651163	37
TAATACG	90	9.1913535E-8	20.555557	27
CAGCCGC	100	1.2430974E-8	20.349998	18
GGTAATA	110	1.6807462E-9	20.181818	25
TACCACG	75	9.081705E-6	19.733332	4
>>END_MODULE