FastQCFastQC Report
Fri 10 Feb 2017
ERR1632656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062262
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA34110.16540090444376127No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA28410.13776135137048542No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22160.1074548238778584No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA20820.10095710438343915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.029.7653621
ATACGGC2750.024.21818429
TAATAGT502.7031105E-422.24
CTTATAC25800.021.43992237
GCGGTAA14650.020.70989823
TACGGCT3450.019.8405830
GGTAATA16250.019.69538525
TATACAC6200.019.69354837
TAATACG15000.019.48666827
CGGTAAT15700.019.44267524
GTATCAA27750.019.1333332
AGTATTA2150.018.93023315
AACTCCG16850.018.5548955
CGCGGTA17100.018.522
TTAGACT609.240209E-418.54
GTATATA500.007036729718.51
GCTAACT16700.018.52
TCTATAC1002.8776776E-718.53
CTAACTC16700.018.3892213
CGTGCCA17400.018.28735510
Produced by FastQC (version 0.11.5)