##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632656.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2062262
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08937855616794	34.0	33.0	34.0	31.0	34.0
2	33.233588166779974	34.0	33.0	34.0	31.0	34.0
3	33.307679140671745	34.0	34.0	34.0	31.0	34.0
4	36.57873490371252	37.0	37.0	37.0	35.0	37.0
5	36.547091494679144	37.0	37.0	37.0	35.0	37.0
6	36.58718678809967	37.0	37.0	37.0	35.0	37.0
7	36.58447617228073	37.0	37.0	37.0	35.0	37.0
8	36.56031629346805	37.0	37.0	37.0	35.0	37.0
9	38.35167064126673	39.0	39.0	39.0	37.0	39.0
10	38.392947646807244	39.0	39.0	39.0	37.0	39.0
11	38.45506487536501	39.0	39.0	39.0	37.0	39.0
12	38.42720323605827	39.0	39.0	39.0	37.0	39.0
13	38.45105132131611	39.0	39.0	39.0	37.0	39.0
14	40.03906729600798	41.0	40.0	41.0	38.0	41.0
15	40.03075991314392	41.0	40.0	41.0	38.0	41.0
16	40.004630352496434	41.0	40.0	41.0	38.0	41.0
17	39.999016128891476	41.0	40.0	41.0	38.0	41.0
18	39.99173868305773	41.0	40.0	41.0	38.0	41.0
19	40.00044999132021	41.0	40.0	41.0	38.0	41.0
20	39.972318260240456	41.0	40.0	41.0	38.0	41.0
21	39.94265811036619	41.0	40.0	41.0	38.0	41.0
22	39.92054307357649	41.0	40.0	41.0	38.0	41.0
23	39.88691834500175	41.0	40.0	41.0	38.0	41.0
24	39.87060325021748	41.0	40.0	41.0	38.0	41.0
25	39.83261826091932	41.0	40.0	41.0	38.0	41.0
26	39.75837211760678	41.0	40.0	41.0	38.0	41.0
27	39.689662613188815	41.0	40.0	41.0	38.0	41.0
28	39.647922523908214	41.0	40.0	41.0	37.0	41.0
29	39.6165608443544	41.0	40.0	41.0	37.0	41.0
30	39.57189484168355	41.0	40.0	41.0	37.0	41.0
31	39.514232915119415	41.0	40.0	41.0	37.0	41.0
32	39.463975479352285	41.0	40.0	41.0	37.0	41.0
33	39.41235594701352	41.0	39.0	41.0	36.0	41.0
34	39.37303116674797	41.0	39.0	41.0	36.0	41.0
35	39.3042620190839	41.0	39.0	41.0	36.0	41.0
36	39.26287978927993	41.0	39.0	41.0	36.0	41.0
37	39.212281465691554	41.0	39.0	41.0	35.0	41.0
38	39.13761394042076	41.0	39.0	41.0	35.0	41.0
39	39.06086423548511	41.0	39.0	41.0	35.0	41.0
40	38.99516065368998	41.0	39.0	41.0	35.0	41.0
41	38.94239529215977	41.0	39.0	41.0	35.0	41.0
42	38.88207414964733	40.0	39.0	41.0	35.0	41.0
43	38.174842478792705	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	0.0
16	5.0
17	8.0
18	24.0
19	66.0
20	108.0
21	163.0
22	397.0
23	729.0
24	1077.0
25	1624.0
26	2245.0
27	3107.0
28	4214.0
29	5881.0
30	7969.0
31	10656.0
32	14044.0
33	18869.0
34	28312.0
35	44616.0
36	72084.0
37	139498.0
38	366197.0
39	1340366.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.136371615245785	16.480980593154506	12.509710211408636	27.87293758019107
2	20.660905355381615	19.15648933064761	32.956336294806384	27.226269019164395
3	21.10876309605666	19.807812974297157	28.78897055757222	30.294453372073963
4	15.897010176204576	15.28612756284119	32.928211837293226	35.88865042366101
5	17.146802879556525	33.2148388517075	32.967586077811646	16.670772190924335
6	35.67112229192993	32.97864189904096	14.94014824498536	16.410087564043753
7	30.90402674344967	27.66845337789282	19.627234560885086	21.800285317772428
8	27.43167453989842	31.575085997802415	19.721645455330115	21.271594006969046
9	27.681012402885763	13.533682917107527	17.505438203293277	41.27986647671344
10	19.037105857548653	23.978524552166505	30.204067184479953	26.78030240580489
11	38.92114580979526	19.652886005754848	19.18267417040124	22.24329401404865
12	23.092846592721973	23.44236571298894	26.585661763636242	26.879125930652847
13	33.77514593199118	17.828820974250604	21.514870564457862	26.88116252930035
14	24.234845039088146	19.302106133944182	22.745461051990485	33.71758777497719
15	28.91868249524066	24.41663571359992	20.24941544769772	26.415266343461695
16	26.481067875953684	23.85206147424527	22.00772743715396	27.659143212647088
17	26.768567718359744	23.693546212847835	22.029742098724604	27.508143970067817
18	26.109970508111967	22.21715766473901	23.75352889206124	27.919342935087784
19	28.144435576081023	22.5431589196717	23.185026926743546	26.12737857750373
20	28.87019205125246	21.51734357710126	23.123589534210492	26.488874837435787
21	27.164977097963305	22.758262529203368	22.058012027569728	28.0187483452636
22	27.861008930969973	22.913480440409607	21.510118500947016	27.7153921276734
23	27.520751485504753	22.828525182542275	22.53506101552567	27.115662316427304
24	28.067190298807816	22.23252913548327	22.2484340011114	27.451846564597517
25	28.21489219119588	22.253137574178254	22.874882046994998	26.657088187630862
26	27.8670702364685	22.701674181069137	22.4535485791815	26.977707003280866
27	27.355302090617002	22.135499757062878	23.397802994963783	27.111395157356338
28	26.40396807001244	22.9990175836048	22.59829255448629	27.998721791896468
29	27.161679747772105	23.563834275179392	22.342990366888397	26.931495610160106
30	26.751014177636012	22.96875954655616	23.369678537450625	26.910547738357206
31	27.477546499911266	22.507712405116322	22.82629462211882	27.188446472853595
32	25.356477498979274	22.56939224986932	23.356392155797856	28.71773809535355
33	26.172814123520677	22.056896747358	24.125644559226714	27.64464456989461
34	26.341463887711647	21.886452836739466	24.23145070800897	27.540632567539912
35	26.088149808317272	22.75714724899164	24.323437080254596	26.83126586243649
36	25.921488152329818	21.765954083428777	25.17119551250035	27.141362251741047
37	26.399555439609518	20.93172448505573	24.876955498379935	27.79176457695482
38	25.29436124023039	20.694169799957525	26.415363324349673	27.596105635462422
39	24.7763863175484	19.82677273789654	27.482831958305976	27.91400898624908
40	24.415811376052122	20.542103767610516	28.37660782189654	26.66547703444082
41	22.981561023769046	20.237244346256684	29.240562062434357	27.540632567539912
42	22.03420321957152	20.650964814364034	29.700881847214372	27.613950118850077
43	21.253991975801327	21.051156448598675	29.57189726620575	28.12295430939425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.5
2	19.0
3	48.5
4	78.0
5	78.0
6	96.0
7	114.0
8	109.5
9	105.0
10	194.5
11	284.0
12	284.0
13	537.5
14	791.0
15	1448.0
16	2105.0
17	2092.5
18	2080.0
19	2080.0
20	2465.5
21	2851.0
22	2883.5
23	2916.0
24	3572.0
25	4228.0
26	4228.0
27	5249.5
28	6271.0
29	9683.5
30	13096.0
31	15528.0
32	17960.0
33	17960.0
34	23177.0
35	28394.0
36	32028.0
37	35662.0
38	47797.5
39	59933.0
40	59933.0
41	70255.5
42	80578.0
43	88578.5
44	96579.0
45	119207.5
46	141836.0
47	141836.0
48	160047.5
49	178259.0
50	197966.0
51	217673.0
52	228318.5
53	238964.0
54	238964.0
55	218517.0
56	198070.0
57	186316.5
58	174563.0
59	161662.0
60	148761.0
61	148761.0
62	137568.0
63	126375.0
64	107452.5
65	88530.0
66	77108.5
67	65687.0
68	65687.0
69	55985.0
70	46283.0
71	39617.5
72	32952.0
73	26754.0
74	20556.0
75	20556.0
76	16545.5
77	12535.0
78	10261.0
79	7987.0
80	6185.0
81	4383.0
82	4383.0
83	3404.0
84	2425.0
85	1854.0
86	1283.0
87	972.5
88	662.0
89	662.0
90	469.0
91	276.0
92	191.5
93	107.0
94	70.5
95	34.0
96	34.0
97	20.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2062262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.17229755004399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.65050758780423	36.95035111380701
2	8.495790949116458	7.505572114546857
3	2.5946166721556265	3.4383053901228986
4	1.2853615252450536	2.2710948701001157
5	0.7540791346333574	1.6654703955652173
6	0.5079442011405971	1.3462237434961113
7	0.3653472597268147	1.1296759506022243
8	0.2600485751639892	0.9189554431686968
9	0.21295557014465263	0.8466063128431974
>10	1.4636879893356143	12.859160527054575
>50	0.20595416435039254	6.4068534256498655
>100	0.18130604082981158	16.083624287065863
>500	0.016772661151338516	4.993919436970235
>1k	0.0056276692020938435	3.584186989007196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3411	0.16540090444376127	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2841	0.13776135137048542	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2216	0.1074548238778584	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2082	0.10095710438343915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	4.849044398820324E-5	0.0	0.0	0.0	0.0
5	4.849044398820324E-5	0.0	0.0	0.0	0.0
6	4.849044398820324E-5	0.0	0.0	0.0	0.0
7	4.849044398820324E-5	0.0	0.0	0.0	0.0
8	9.698088797640649E-5	0.0	0.0	0.0	0.0
9	9.698088797640649E-5	0.0	0.0	9.698088797640649E-5	0.0
10	1.4547133196460974E-4	0.0	0.0	9.698088797640649E-5	0.0
11	1.4547133196460974E-4	0.0	0.0	9.698088797640649E-5	0.0
12	1.4547133196460974E-4	0.0	0.0	9.698088797640649E-5	0.0
13	1.4547133196460974E-4	0.0	0.0	2.4245221994101623E-4	0.0
14	1.4547133196460974E-4	0.0	0.0	2.4245221994101623E-4	0.0
15	1.4547133196460974E-4	0.0	0.0	3.394331079174227E-4	0.0
16	1.4547133196460974E-4	0.0	0.0	6.303757718466422E-4	0.0
17	1.4547133196460974E-4	0.0	0.0	0.0012607515436932845	0.0
18	1.9396177595281297E-4	0.0	0.0	0.0014547133196460974	0.0
19	2.9094266392921947E-4	0.0	0.0	0.0016486750955989104	0.0
20	2.9094266392921947E-4	0.0	0.0	0.002569993531374772	0.0
21	2.9094266392921947E-4	0.0	0.0	0.0034428215231624305	0.0
22	2.9094266392921947E-4	0.0	0.0	0.005770362834596186	0.0
23	2.9094266392921947E-4	0.0	0.0	0.0071765857102540805	0.0
24	2.9094266392921947E-4	0.0	0.0	0.009213184357758617	0.0
25	3.394331079174227E-4	0.0	0.0	0.010376955013475495	0.0
26	3.394331079174227E-4	0.0	0.0	0.014304680976519957	0.0
27	3.394331079174227E-4	0.0	0.0	0.03641632343514064	0.0
28	3.394331079174227E-4	0.0	0.0	0.13936153602209614	0.0
29	3.394331079174227E-4	0.0	0.0	0.30228942782245904	0.0
30	3.394331079174227E-4	0.0	0.0	0.5117196554075089	0.0
31	3.394331079174227E-4	0.0	0.0	1.196016800969033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	29.765362	1
ATACGGC	275	0.0	24.218184	29
TAATAGT	50	2.7031105E-4	22.2	4
CTTATAC	2580	0.0	21.439922	37
GCGGTAA	1465	0.0	20.709898	23
TACGGCT	345	0.0	19.84058	30
GGTAATA	1625	0.0	19.695385	25
TATACAC	620	0.0	19.693548	37
TAATACG	1500	0.0	19.486668	27
CGGTAAT	1570	0.0	19.442675	24
GTATCAA	2775	0.0	19.133333	2
AGTATTA	215	0.0	18.930233	15
AACTCCG	1685	0.0	18.554895	5
CGCGGTA	1710	0.0	18.5	22
TTAGACT	60	9.240209E-4	18.5	4
GTATATA	50	0.0070367297	18.5	1
GCTAACT	1670	0.0	18.5	2
TCTATAC	100	2.8776776E-7	18.5	3
CTAACTC	1670	0.0	18.389221	3
CGTGCCA	1740	0.0	18.287355	10
>>END_MODULE