FastQCFastQC Report
Fri 10 Feb 2017
ERR1632655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128914
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2960.22961043796639619No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2420.18772204725631042No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2420.18772204725631042No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2370.18384349256093208No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2340.1815163597437051No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2100.1628992972058892No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2060.15979645344958654No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2060.15979645344958654No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2030.15746932063235958No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1990.1543664768760569No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1950.15126363311975427No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC1810.14040367997269498No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1690.13109514870378702No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1660.12876801588656003No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1660.12876801588656003No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG1590.1233380393130304No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG1590.1233380393130304No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1570.12178661743487906No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1530.11868377367857642No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT1510.1171323518004251TruSeq Adapter, Index 1 (95% over 22bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1510.1171323518004251No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG1420.11015095334874411No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1410.10937524240966845No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC1410.10937524240966845No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA1340.10394526583613882No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1330.10316955489706316No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1300.10084242207983618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATG401.5855239E-632.3751
TCTATGG401.5855239E-632.3752
GTGATTC453.9720762E-628.77777716
GGTGATT453.9720762E-628.77777715
ATATGCC405.8999307E-527.7535
TATGCCC405.8999307E-527.7536
CTTATTG405.8999307E-527.7528
GGTATCA1850.027.01
GTATTAG358.826564E-426.4285721
TATGGGC509.009602E-625.9000024
GCAGTCG451.3143296E-424.6666669
GATATGC451.3143296E-424.66666634
ATTGATA451.3143296E-424.66666631
TTATTGA451.3143296E-424.66666629
GTCGGTG451.3143296E-424.66666612
TAGGAGC802.6784619E-823.1254
TTTAGGA753.6877464E-722.22
TTAGGAG753.6877464E-722.23
CTTTAGG753.6877464E-722.21
GGTTCGG753.6877464E-722.213
Produced by FastQC (version 0.11.5)