##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128914
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05365592565586	34.0	33.0	34.0	31.0	34.0
2	33.192996881642024	34.0	33.0	34.0	31.0	34.0
3	33.26824084273236	34.0	34.0	34.0	31.0	34.0
4	36.548280248848066	37.0	37.0	37.0	35.0	37.0
5	36.52237926059233	37.0	37.0	37.0	35.0	37.0
6	36.562087903563615	37.0	37.0	37.0	35.0	37.0
7	36.5615138774687	37.0	37.0	37.0	35.0	37.0
8	36.52766960919683	37.0	37.0	37.0	35.0	37.0
9	38.336216392323564	39.0	39.0	39.0	37.0	39.0
10	38.368012783716274	39.0	39.0	39.0	37.0	39.0
11	38.44631304590657	39.0	39.0	39.0	37.0	39.0
12	38.395154909474535	39.0	39.0	39.0	37.0	39.0
13	38.43039545743674	39.0	39.0	39.0	37.0	39.0
14	39.98420652528042	41.0	40.0	41.0	38.0	41.0
15	39.97958328808353	41.0	40.0	41.0	38.0	41.0
16	39.95798749553966	41.0	40.0	41.0	38.0	41.0
17	39.94110802550538	41.0	40.0	41.0	38.0	41.0
18	39.94070465581706	41.0	40.0	41.0	38.0	41.0
19	39.918038382177265	41.0	40.0	41.0	38.0	41.0
20	39.92024140124424	41.0	40.0	41.0	38.0	41.0
21	39.887366771646214	41.0	40.0	41.0	38.0	41.0
22	39.87616550568596	41.0	40.0	41.0	38.0	41.0
23	39.83730238763827	41.0	40.0	41.0	38.0	41.0
24	39.81690118994058	41.0	40.0	41.0	38.0	41.0
25	39.76718587585522	41.0	40.0	41.0	38.0	41.0
26	39.67798687497091	41.0	40.0	41.0	38.0	41.0
27	39.60576042943357	41.0	40.0	41.0	37.0	41.0
28	39.55436957971981	41.0	40.0	41.0	37.0	41.0
29	39.51556076143786	41.0	40.0	41.0	37.0	41.0
30	39.4875808678654	41.0	39.0	41.0	37.0	41.0
31	39.41054501450579	41.0	39.0	41.0	36.0	41.0
32	39.37400127216594	41.0	39.0	41.0	36.0	41.0
33	39.29777215818297	41.0	39.0	41.0	36.0	41.0
34	39.24589261057759	41.0	39.0	41.0	36.0	41.0
35	39.18421583381169	41.0	39.0	41.0	35.0	41.0
36	39.11703150937834	41.0	39.0	41.0	35.0	41.0
37	39.04777603673767	41.0	39.0	41.0	35.0	41.0
38	38.97551856276277	41.0	39.0	41.0	35.0	41.0
39	38.89605473416386	40.0	39.0	41.0	35.0	41.0
40	38.79072870285617	40.0	38.0	41.0	35.0	41.0
41	38.74312332252509	40.0	38.0	41.0	35.0	41.0
42	38.6774050917666	40.0	38.0	41.0	35.0	41.0
43	37.953690056937184	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	8.0
21	9.0
22	22.0
23	35.0
24	69.0
25	88.0
26	156.0
27	206.0
28	271.0
29	371.0
30	535.0
31	735.0
32	991.0
33	1364.0
34	2012.0
35	3031.0
36	4773.0
37	9905.0
38	24545.0
39	79785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92773476891571	16.18598445475278	12.346215306328249	27.540065470003256
2	21.23896551189165	19.048357819941977	31.99885194781017	27.713824720356207
3	21.72145771599671	18.76755045999659	28.287850815272197	31.223141008734505
4	15.55998572691872	15.6817723443536	32.782319996276584	35.975921932451094
5	17.85841723939991	32.034534651007654	32.746637293079104	17.360410816513337
6	36.08219433110445	32.332407651612705	15.182214499588872	16.403183517693968
7	31.517911165583257	26.29117085809144	19.530074313107963	22.66084366321734
8	28.180026994740683	29.961835021797476	20.15529733000295	21.702840653458896
9	28.48022712816296	12.041360907271514	17.360410816513337	42.118001148052194
10	19.405184851916783	22.798920210372806	29.47468855205796	28.321206385652452
11	40.44324123058784	18.63102533471927	17.821958825263355	23.103774609429543
12	22.888126968366507	23.138681601687946	25.961493708984285	28.01169772096126
13	35.90067797136075	16.854647284236002	20.463254572815988	26.78142017158726
14	25.08959461346324	19.565757016305444	22.44985028778876	32.89479808244256
15	29.665513443070573	23.60488387607242	19.864405727849572	26.86519695300743
16	27.350792000868797	22.625936670958936	22.042602044774036	27.980669283398235
17	27.605225188885612	22.750826132150117	21.169927238313914	28.474021440650354
18	27.20650976620072	20.405076252385314	23.00370789828878	29.384706083125185
19	29.4607257551546	22.14422017779295	22.269885349923204	26.12516871712925
20	30.828304140744994	20.15064306436849	22.128705959011434	26.89234683587508
21	27.7619187985789	22.14344446685387	20.949625331616424	29.145011402950804
22	29.4925299036567	21.944086755511428	20.468684549389515	28.094698791442358
23	28.031866205377227	21.5624369734862	21.513567184324433	28.892129636812136
24	28.22812107296337	21.352995019935772	21.8525528647005	28.56633104240036
25	28.832399894503315	21.309555207347533	22.36917635012489	27.488868548024264
26	28.765688753742808	22.2349783576648	21.08537474595467	27.91395814263773
27	28.06677319763563	21.553128442217293	23.158850086103914	27.22124827404316
28	26.802364366942307	22.449074576849686	21.694307833129063	29.054253223078952
29	28.34758055758102	23.06809190623206	21.70206494251982	26.882262593667093
30	28.017903408473867	20.95505530818996	22.62128240532448	28.4057588780117
31	27.73864747040663	21.370060660595435	22.14189304497572	28.749398824022215
32	26.108878787408663	21.767224661402178	22.14422017779295	29.97967637339622
33	26.57508106179313	21.010906495803404	23.976449415889665	28.4375630265138
34	27.387250415005354	21.069860527173155	23.373722016227873	28.169167041593617
35	26.936562359402394	22.053461997921094	23.827512915587135	27.18246272708938
36	26.462602975627163	21.136571667933662	23.843802845307724	28.557022511131454
37	26.073971795150253	20.939541089408443	25.184231348030472	27.802255767410834
38	25.635695114572503	20.264672572412618	25.12217447290442	28.97745784011046
39	25.078734660316183	19.641000977395784	27.605225188885612	27.67503917340242
40	25.537955536248973	20.141334533099588	27.53773833718603	26.782971593465412
41	23.12549451572366	19.88534992320462	28.302589323114635	28.686566237957088
42	21.075290503746682	20.931783980017688	29.798160013652513	28.19476550258312
43	21.39565912158493	20.706827807685745	28.64622926912515	29.25128380160417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	3.0
4	3.0
5	3.0
6	5.0
7	7.0
8	6.0
9	5.0
10	10.5
11	16.0
12	16.0
13	33.0
14	50.0
15	84.5
16	119.0
17	110.5
18	102.0
19	102.0
20	124.5
21	147.0
22	115.5
23	84.0
24	115.0
25	146.0
26	146.0
27	178.5
28	211.0
29	282.5
30	354.0
31	429.0
32	504.0
33	504.0
34	860.0
35	1216.0
36	1347.0
37	1478.0
38	1879.0
39	2280.0
40	2280.0
41	2755.5
42	3231.0
43	4079.0
44	4927.0
45	6719.5
46	8512.0
47	8512.0
48	9759.0
49	11006.0
50	12427.0
51	13848.0
52	14532.0
53	15216.0
54	15216.0
55	14475.0
56	13734.0
57	12737.5
58	11741.0
59	11462.0
60	11183.0
61	11183.0
62	10221.0
63	9259.0
64	7396.5
65	5534.0
66	4990.0
67	4446.0
68	4446.0
69	3895.0
70	3344.0
71	3240.0
72	3136.0
73	2490.0
74	1844.0
75	1844.0
76	1192.0
77	540.0
78	419.5
79	299.0
80	247.0
81	195.0
82	195.0
83	143.5
84	92.0
85	75.0
86	58.0
87	41.5
88	25.0
89	25.0
90	19.0
91	13.0
92	6.5
93	0.0
94	1.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	128914.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.870906185519026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.58581531148215	44.72128705959012
2	6.929487367587077	7.327365530508711
3	2.4135097860852723	3.828133484338396
4	1.3791484491915842	2.9166731309244924
5	0.8773731623580505	2.319375707836232
6	0.6015434725197335	1.9082489101261306
7	0.4064086387511371	1.5041035108677103
8	0.3183778866750785	1.3466341902353507
9	0.24942046421549927	1.186837736785764
>10	1.9704216673024442	20.093240454876895
>50	0.19953637137239943	7.306421335153668
>100	0.06895742245957921	5.541678948756535
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	296	0.22961043796639619	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	242	0.18772204725631042	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	242	0.18772204725631042	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	237	0.18384349256093208	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	234	0.1815163597437051	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	210	0.1628992972058892	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	206	0.15979645344958654	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	206	0.15979645344958654	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	203	0.15746932063235958	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	199	0.1543664768760569	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	195	0.15126363311975427	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	181	0.14040367997269498	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	169	0.13109514870378702	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	166	0.12876801588656003	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	166	0.12876801588656003	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	159	0.1233380393130304	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	159	0.1233380393130304	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	157	0.12178661743487906	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	153	0.11868377367857642	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	151	0.1171323518004251	TruSeq Adapter, Index 1 (95% over 22bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	151	0.1171323518004251	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	142	0.11015095334874411	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	141	0.10937524240966845	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	141	0.10937524240966845	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	134	0.10394526583613882	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	133	0.10316955489706316	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	130	0.10084242207983618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	7.757109390756628E-4	0.0	0.0	0.0
5	0.0	7.757109390756628E-4	0.0	0.0	0.0
6	0.0	7.757109390756628E-4	0.0	0.0	0.0
7	0.0	7.757109390756628E-4	0.0	0.0	0.0
8	0.0	7.757109390756628E-4	0.0	0.0	0.0
9	0.0	7.757109390756628E-4	0.0	0.0	0.0
10	0.0	7.757109390756628E-4	0.0	0.0	0.0
11	0.0	7.757109390756628E-4	0.0	0.0	0.0
12	0.0	7.757109390756628E-4	0.0	0.0	0.0
13	0.0	7.757109390756628E-4	0.0	0.0	0.0
14	0.0	7.757109390756628E-4	0.0	0.0	0.0
15	0.0	7.757109390756628E-4	0.0	0.0	0.0
16	0.0	7.757109390756628E-4	0.0	0.0	0.0
17	0.0	7.757109390756628E-4	0.0	7.757109390756628E-4	0.0
18	0.0	7.757109390756628E-4	0.0	7.757109390756628E-4	0.0
19	0.0	7.757109390756628E-4	0.0	7.757109390756628E-4	0.0
20	0.0	7.757109390756628E-4	0.0	7.757109390756628E-4	0.0
21	0.0	7.757109390756628E-4	0.0	0.004654265634453977	0.0
22	0.0	7.757109390756628E-4	0.0	0.007757109390756628	0.0
23	0.0	7.757109390756628E-4	0.0	0.008532820329832292	0.0
24	0.0	7.757109390756628E-4	0.0	0.009308531268907953	0.0
25	0.0	7.757109390756628E-4	0.0	0.012411375025210605	0.0
26	0.0	7.757109390756628E-4	0.0	0.01628992972058892	0.0
27	0.0	7.757109390756628E-4	0.0	0.04266410164916146	0.0
28	0.0	7.757109390756628E-4	0.0	0.14738507842437595	0.0
29	0.0	7.757109390756628E-4	0.0	0.3343314147416107	0.0
30	0.0	7.757109390756628E-4	0.0	0.5802317824285959	0.0
31	0.0	7.757109390756628E-4	0.0	1.255876010363498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTATG	40	1.5855239E-6	32.375	1
TCTATGG	40	1.5855239E-6	32.375	2
GTGATTC	45	3.9720762E-6	28.777777	16
GGTGATT	45	3.9720762E-6	28.777777	15
ATATGCC	40	5.8999307E-5	27.75	35
TATGCCC	40	5.8999307E-5	27.75	36
CTTATTG	40	5.8999307E-5	27.75	28
GGTATCA	185	0.0	27.0	1
GTATTAG	35	8.826564E-4	26.428572	1
TATGGGC	50	9.009602E-6	25.900002	4
GCAGTCG	45	1.3143296E-4	24.666666	9
GATATGC	45	1.3143296E-4	24.666666	34
ATTGATA	45	1.3143296E-4	24.666666	31
TTATTGA	45	1.3143296E-4	24.666666	29
GTCGGTG	45	1.3143296E-4	24.666666	12
TAGGAGC	80	2.6784619E-8	23.125	4
TTTAGGA	75	3.6877464E-7	22.2	2
TTAGGAG	75	3.6877464E-7	22.2	3
CTTTAGG	75	3.6877464E-7	22.2	1
GGTTCGG	75	3.6877464E-7	22.2	13
>>END_MODULE