FastQCFastQC Report
Fri 10 Feb 2017
ERR1632654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT390.7332205301748449RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA190.35721000188005264No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT170.3196089490505734No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT160.3008084226358338No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC150.2820078962210942No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA150.2820078962210942No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG150.2820078962210942No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA140.26320736980635456No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC130.24440684339161498No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA130.24440684339161498No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA120.2256063169768754No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC120.2256063169768754No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA120.2256063169768754No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC110.20680579056213572RNA PCR Primer, Index 29 (95% over 21bp)
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA110.20680579056213572No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA100.18800526414739613No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT100.18800526414739613No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC100.18800526414739613No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA100.18800526414739613No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA100.18800526414739613No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT100.18800526414739613No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA90.1692047377326565No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC90.1692047377326565No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA90.1692047377326565No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC90.1692047377326565No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA90.1692047377326565No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC90.1692047377326565No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA90.1692047377326565No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT80.1504042113179169No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT80.1504042113179169No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG80.1504042113179169No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT80.1504042113179169No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA80.1504042113179169No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC80.1504042113179169No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA70.13160368490317728No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC70.13160368490317728No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG70.13160368490317728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.13160368490317728No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG70.13160368490317728No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA70.13160368490317728No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC70.13160368490317728No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG70.13160368490317728No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT70.13160368490317728No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC70.13160368490317728No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT70.13160368490317728No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA70.13160368490317728No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT60.1128031584884377No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC60.1128031584884377No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC60.1128031584884377No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA60.1128031584884377No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG60.1128031584884377No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG60.1128031584884377No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT60.1128031584884377No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC60.1128031584884377No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC60.1128031584884377No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT60.1128031584884377No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.1128031584884377No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT60.1128031584884377No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA60.1128031584884377No Hit
TCGCAGATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCA60.1128031584884377No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC60.1128031584884377No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA60.1128031584884377No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC60.1128031584884377No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC60.1128031584884377No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA60.1128031584884377No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG60.1128031584884377No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG60.1128031584884377No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT60.1128031584884377No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC60.1128031584884377No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)