FastQCFastQC Report
Fri 10 Feb 2017
ERR1632653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13701
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1140.8320560543026055TruSeq Adapter, Index 8 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA460.33574191664841985No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA310.22626085687176117No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA310.22626085687176117No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT290.21166338223487335No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC280.20436464491642944No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC270.19706590759798556RNA PCR Primer, Index 8 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA260.18976717027954162No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.17516969564265383No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA230.16787095832420992No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT220.160572221005766No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC210.15327348368732208No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT210.15327348368732208No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA200.14597474636887817No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC190.13867600905043428No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT190.13867600905043428No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA190.13867600905043428No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA180.13137727173199037No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG180.13137727173199037No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC170.12407853441354647No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC160.11677979709510254No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA150.10948105977665863No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA150.10948105977665863No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC150.10948105977665863No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA150.10948105977665863No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC150.10948105977665863No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT150.10948105977665863No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA140.10218232245821472No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT140.10218232245821472No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC140.10218232245821472No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT140.10218232245821472No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC140.10218232245821472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTT854.900068E-415.23529437
TGTCTCT1000.001677198712.95000136
Produced by FastQC (version 0.11.5)