##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632653.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13701
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9845266768849	34.0	33.0	34.0	31.0	34.0
2	33.11641486022918	34.0	33.0	34.0	31.0	34.0
3	33.20020436464492	34.0	33.0	34.0	31.0	34.0
4	36.49113203415809	37.0	37.0	37.0	35.0	37.0
5	36.444639077439604	37.0	37.0	37.0	35.0	37.0
6	36.4985767462229	37.0	37.0	37.0	35.0	37.0
7	36.49514633968324	37.0	37.0	37.0	35.0	37.0
8	36.48332238522735	37.0	37.0	37.0	35.0	37.0
9	38.26727976060142	39.0	39.0	39.0	37.0	39.0
10	38.28012553828188	39.0	39.0	39.0	37.0	39.0
11	38.372016641121085	39.0	39.0	39.0	37.0	39.0
12	38.30902853806292	39.0	39.0	39.0	37.0	39.0
13	38.34763885847748	39.0	39.0	39.0	37.0	39.0
14	39.8776001751697	41.0	40.0	41.0	38.0	41.0
15	39.872199109554046	41.0	40.0	41.0	38.0	41.0
16	39.881249543828915	41.0	40.0	41.0	38.0	41.0
17	39.8517626450624	41.0	40.0	41.0	38.0	41.0
18	39.86884169038756	41.0	40.0	41.0	38.0	41.0
19	39.829209546748416	41.0	40.0	41.0	38.0	41.0
20	39.76206116341873	41.0	40.0	41.0	38.0	41.0
21	39.734909860594115	41.0	40.0	41.0	38.0	41.0
22	39.729435807605284	41.0	40.0	41.0	38.0	41.0
23	39.66287132326108	41.0	40.0	41.0	37.0	41.0
24	39.656740383913586	41.0	40.0	41.0	37.0	41.0
25	39.60032114444201	41.0	40.0	41.0	37.0	41.0
26	39.49587621341508	41.0	40.0	41.0	37.0	41.0
27	39.42646522151668	41.0	39.0	41.0	37.0	41.0
28	39.417706736734544	41.0	39.0	41.0	37.0	41.0
29	39.33793153784395	41.0	39.0	41.0	36.0	41.0
30	39.27706006860813	41.0	39.0	41.0	36.0	41.0
31	39.222246551346615	41.0	39.0	41.0	36.0	41.0
32	39.17984088752646	41.0	39.0	41.0	35.0	41.0
33	39.0745930953945	41.0	39.0	41.0	35.0	41.0
34	39.00941537114079	41.0	39.0	41.0	35.0	41.0
35	38.9329246040435	40.0	39.0	41.0	35.0	41.0
36	38.890883877089266	40.0	39.0	41.0	35.0	41.0
37	38.82753083716517	40.0	39.0	41.0	35.0	41.0
38	38.75928764323772	40.0	38.0	41.0	35.0	41.0
39	38.64170498503759	40.0	38.0	41.0	35.0	41.0
40	38.610101452448724	40.0	38.0	41.0	35.0	41.0
41	38.562075760893364	40.0	38.0	41.0	35.0	41.0
42	38.49740894825195	40.0	38.0	41.0	35.0	41.0
43	37.77359316838187	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	1.0
22	4.0
23	7.0
24	11.0
25	17.0
26	26.0
27	25.0
28	33.0
29	61.0
30	63.0
31	78.0
32	112.0
33	172.0
34	225.0
35	382.0
36	606.0
37	1092.0
38	2823.0
39	7961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94577038172396	15.714181446609738	12.656010510181739	28.684037661484563
2	22.261148821253922	19.297861469965696	29.435807605284285	29.005182103496097
3	22.458214728851907	19.202977884825927	27.019925552879354	31.318881833442813
4	15.998832202029048	15.071892562586672	32.056054302605645	36.87322093277863
5	18.72126122180863	31.57433763958835	31.880884606962994	17.823516531640028
6	36.8513247208233	32.45018611780162	14.144952923144297	16.553536238230787
7	31.099919713889495	26.428727830085396	19.15918546091526	23.312166995109845
8	28.35559448215459	29.37011896941829	19.56791475074812	22.706371797679
9	28.100138676009053	11.692577184147144	16.531640026275454	43.67564411356835
10	20.21750237208963	21.86701700605795	28.538062915115685	29.377417706736736
11	41.27435953580031	18.283336982701993	16.93307057878987	23.509232902707833
12	22.998321290416758	23.6041164878476	25.830231369972996	27.567330851762645
13	36.79293482227575	17.006057951974306	18.72855995912707	27.472447266622872
14	25.501788190643023	19.04240566382016	21.45098897890665	34.004817166630176
15	30.77877527187797	23.158893511422523	18.151959710970004	27.910371505729508
16	27.61112327567331	22.837749069410993	20.728413984380705	28.822713670535
17	28.282607108970147	22.983723815779868	20.144514998905187	28.58915407634479
18	27.625720750310194	20.305087219910956	21.45098897890665	30.6182030508722
19	30.574410626961534	21.268520545945552	21.75023720896285	26.40683161813006
20	30.764177797241075	19.443836216334574	21.159039486168894	28.632946500255457
21	28.72783008539523	20.232099846726516	21.531275089409533	29.50879497846873
22	29.304430333552293	21.874315743376396	19.80877308225677	29.012480840814543
23	29.50879497846873	21.232026859353333	20.531348076782717	28.72783008539523
24	29.610977300926937	20.772206408291368	20.68462156047004	28.932194730311657
25	30.93204875556529	21.07875337566601	20.246697321363406	27.742500547405296
26	29.786146996569595	21.932705641923945	20.757608933654478	27.523538427851978
27	28.581855339026347	21.85971826873951	22.38522735566747	27.173199036566675
28	27.472447266622872	22.691774323042115	20.735712721699144	29.10006568863587
29	27.98335887891395	22.341434931756808	21.034960951755348	28.640245237573897
30	27.57462958908109	21.275819283263996	23.27567330851763	27.873877819137288
31	29.80074447120648	21.465586453543537	20.845193781475803	27.888475293774178
32	27.976060141595504	20.524049339464273	21.414495292314427	30.085395226625796
33	27.187796511203562	20.94737610393402	23.290270783154515	28.574556601707908
34	27.494343478578205	20.94737610393402	22.399824830304357	29.15845558718342
35	27.304576308298667	21.69914604773374	24.341288957010438	26.654988686957154
36	28.246113422377928	20.837895044157364	22.939931391869205	27.976060141595504
37	27.910371505729508	20.633530399240932	23.27567330851763	28.18042478651193
38	26.69878111086782	19.910955404714983	24.954382891759725	28.43588059265747
39	26.231661922487408	19.582512225385006	25.494489453324576	28.691336398803006
40	25.706152835559447	19.42194000437924	27.16590029924823	27.706006860813083
41	23.91796219254069	19.19567914750748	27.800890445952852	29.08546821399898
42	21.604262462593972	20.341580906503175	30.326253558134443	27.727903072768413
43	21.560470038683306	20.32698343186629	28.90299978103788	29.209546748412524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	4.5
16	8.0
17	7.0
18	6.0
19	6.0
20	7.5
21	9.0
22	7.5
23	6.0
24	8.5
25	11.0
26	11.0
27	13.0
28	15.0
29	26.5
30	38.0
31	45.0
32	52.0
33	52.0
34	71.5
35	91.0
36	107.5
37	124.0
38	161.5
39	199.0
40	199.0
41	272.5
42	346.0
43	393.5
44	441.0
45	611.5
46	782.0
47	782.0
48	884.0
49	986.0
50	1149.0
51	1312.0
52	1376.5
53	1441.0
54	1441.0
55	1510.0
56	1579.0
57	1523.5
58	1468.0
59	1362.5
60	1257.0
61	1257.0
62	1183.0
63	1109.0
64	930.5
65	752.0
66	657.0
67	562.0
68	562.0
69	473.0
70	384.0
71	324.0
72	264.0
73	215.0
74	166.0
75	166.0
76	130.0
77	94.0
78	85.0
79	76.0
80	58.0
81	40.0
82	40.0
83	36.5
84	33.0
85	26.0
86	19.0
87	16.5
88	14.0
89	14.0
90	11.5
91	9.0
92	6.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	13701.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.91394788701554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.6677765445801	71.54952193270564
2	5.114687384387717	8.072403474198964
3	1.368849426563078	3.2406393693890956
4	0.9896411394746578	3.123859572293993
5	0.5364409914909359	2.1166338223487338
6	0.3699593044765076	1.751696956426538
7	0.1664816870144284	0.9196409021239326
8	0.13873473917869034	0.875848478213269
9	0.12948575656677763	0.9196409021239326
>10	0.5086940436551979	6.598058535873294
>50	0.0	0.0
>100	0.00924898261191269	0.8320560543026055
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	114	0.8320560543026055	TruSeq Adapter, Index 8 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	46	0.33574191664841985	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	31	0.22626085687176117	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	31	0.22626085687176117	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	29	0.21166338223487335	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	28	0.20436464491642944	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	27	0.19706590759798556	RNA PCR Primer, Index 8 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	26	0.18976717027954162	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	24	0.17516969564265383	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	23	0.16787095832420992	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	22	0.160572221005766	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	21	0.15327348368732208	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	21	0.15327348368732208	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	20	0.14597474636887817	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	19	0.13867600905043428	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	19	0.13867600905043428	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	19	0.13867600905043428	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	18	0.13137727173199037	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	18	0.13137727173199037	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	17	0.12407853441354647	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	16	0.11677979709510254	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	15	0.10948105977665863	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	15	0.10948105977665863	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	15	0.10948105977665863	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	15	0.10948105977665863	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	15	0.10948105977665863	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	15	0.10948105977665863	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	14	0.10218232245821472	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	14	0.10218232245821472	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	14	0.10218232245821472	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	14	0.10218232245821472	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	14	0.10218232245821472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.080286110502883	0.0
29	0.0	0.0	0.0	0.27735201810086857	0.0
30	0.0	0.0	0.0	0.401430552514415	0.0
31	0.0	0.0	0.0	0.9269396394423765	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTCTT	85	4.900068E-4	15.235294	37
TGTCTCT	100	0.0016771987	12.950001	36
>>END_MODULE