ERR1632652.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632652.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23395
Sequences flagged as poor quality	0
Sequence length	43
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	104	0.4445394315024578	TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	72	0.30775806796324	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	63	0.269288309467835	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	63	0.269288309467835	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	52	0.2222697157512289	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	51	0.21799529814062832	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	50	0.21372088053002777	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	47	0.20089762769822614	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	43	0.1837999572558239	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	43	0.1837999572558239	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	42	0.17952553964522333	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	41	0.17525112203462279	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	40	0.1709767044240222	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	39	0.16670228681342167	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	35	0.14960461637101946	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	34	0.1453301987604189	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	34	0.1453301987604189	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	32	0.13678136353921777	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	32	0.13678136353921777	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	31	0.13250694592861723	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	31	0.13250694592861723	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	31	0.13250694592861723	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	31	0.13250694592861723	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	31	0.13250694592861723	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	30	0.12823252831801668	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	30	0.12823252831801668	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	29	0.12395811070741612	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	29	0.12395811070741612	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	29	0.12395811070741612	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	28	0.11968369309681556	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	28	0.11968369309681556	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	28	0.11968369309681556	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	28	0.11968369309681556	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	27	0.115409275486215	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	27	0.115409275486215	RNA PCR Primer, Index 6 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	26	0.11113485787561445	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	26	0.11113485787561445	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	26	0.11113485787561445	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	24	0.10258602265441334	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	35	2.290378E-5	31.714285	1
TACGGAG	25	0.005388136	29.6	30
GTATGCC	45	1.2698074E-4	24.666668	32
TGCCGTC	45	1.2698074E-4	24.666668	35
CCGTCTT	45	1.2698074E-4	24.666668	37
ATGCCGT	45	1.2698074E-4	24.666668	34
CGTATGC	45	1.2698074E-4	24.666668	31
GCCGTCT	45	1.2698074E-4	24.666668	36
CGCGGTA	40	0.001875412	23.125	22
TAATACG	40	0.001875412	23.125	27
AATACGG	40	0.001875412	23.125	28
GTAATAC	40	0.001875412	23.125	26
CCGCGGT	40	0.001875412	23.125	21
AGCCCAC	50	2.5939112E-4	22.2	7
ATCTCGT	50	2.5939112E-4	22.2	27
GCCCACG	50	2.5939112E-4	22.2	8
TATGCCG	50	2.5939112E-4	22.2	33
GAGCCCA	50	2.5939112E-4	22.2	6
CACGAGA	50	2.5939112E-4	22.2	11
TCTCGTA	50	2.5939112E-4	22.2	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content