##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632652.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23395
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03859799102372	34.0	33.0	34.0	31.0	34.0
2	33.171275913656764	34.0	33.0	34.0	31.0	34.0
3	33.249027569993586	34.0	34.0	34.0	31.0	34.0
4	36.53720880530028	37.0	37.0	37.0	35.0	37.0
5	36.50502244069246	37.0	37.0	37.0	35.0	37.0
6	36.55832442829664	37.0	37.0	37.0	35.0	37.0
7	36.557597777302846	37.0	37.0	37.0	35.0	37.0
8	36.51985466980124	37.0	37.0	37.0	35.0	37.0
9	38.32207736695875	39.0	39.0	39.0	37.0	39.0
10	38.33990168839495	39.0	39.0	39.0	37.0	39.0
11	38.411626415900834	39.0	39.0	39.0	37.0	39.0
12	38.37183158794614	39.0	39.0	39.0	37.0	39.0
13	38.40474460354777	39.0	39.0	39.0	37.0	39.0
14	39.96148749732849	41.0	40.0	41.0	38.0	41.0
15	39.968924983970936	41.0	40.0	41.0	38.0	41.0
16	39.920795041675575	41.0	40.0	41.0	38.0	41.0
17	39.90228681342167	41.0	40.0	41.0	38.0	41.0
18	39.924684761701215	41.0	40.0	41.0	38.0	41.0
19	39.91690532164993	41.0	40.0	41.0	38.0	41.0
20	39.87933319085275	41.0	40.0	41.0	38.0	41.0
21	39.85565291729002	41.0	40.0	41.0	38.0	41.0
22	39.81996153024151	41.0	40.0	41.0	38.0	41.0
23	39.773199401581536	41.0	40.0	41.0	38.0	41.0
24	39.778328702714255	41.0	40.0	41.0	38.0	41.0
25	39.74366317589229	41.0	40.0	41.0	38.0	41.0
26	39.66783500748023	41.0	40.0	41.0	37.0	41.0
27	39.562385125026715	41.0	40.0	41.0	37.0	41.0
28	39.49728574481727	41.0	40.0	41.0	37.0	41.0
29	39.450566360333404	41.0	40.0	41.0	37.0	41.0
30	39.42415045949989	41.0	39.0	41.0	36.0	41.0
31	39.35811070741612	41.0	39.0	41.0	36.0	41.0
32	39.25659328916435	41.0	39.0	41.0	36.0	41.0
33	39.21017311391323	41.0	39.0	41.0	35.0	41.0
34	39.16247061337893	41.0	39.0	41.0	35.0	41.0
35	39.080530027783716	41.0	39.0	41.0	35.0	41.0
36	39.02397948279547	41.0	39.0	41.0	35.0	41.0
37	38.951228895063046	41.0	39.0	41.0	35.0	41.0
38	38.84714682624492	40.0	39.0	41.0	35.0	41.0
39	38.760034195340886	40.0	38.0	41.0	35.0	41.0
40	38.65330198760419	40.0	38.0	41.0	35.0	41.0
41	38.609147253686686	40.0	38.0	41.0	35.0	41.0
42	38.536054712545415	40.0	38.0	41.0	35.0	41.0
43	37.78298781790981	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	3.0
22	6.0
23	11.0
24	14.0
25	26.0
26	25.0
27	43.0
28	49.0
29	80.0
30	92.0
31	130.0
32	193.0
33	237.0
34	382.0
35	622.0
36	939.0
37	1792.0
38	4682.0
39	14063.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.808292370164565	16.1444753152383	13.14810857020731	27.899123744389826
2	22.120111134857876	18.790339816200046	30.412481299422957	28.677067749519132
3	21.735413549903825	19.07245137849968	28.117119042530454	31.075016029066038
4	16.1530241504595	15.15708484718957	33.23359692241932	35.45629407993161
5	18.409916648856594	31.647787988886517	32.20773669587519	17.734558666381705
6	34.96046163710195	32.3017738833084	15.3750801453302	17.362684334259455
7	30.93396024791622	26.300491558025218	19.2306048300919	23.534943363966658
8	28.18550972430006	29.412267578542423	20.260739474246634	22.14148322291088
9	28.040179525539642	12.194913443043385	16.75999145116478	43.00491558025219
10	19.636674503098952	22.633041248129942	28.429151528104295	29.30113272066681
11	40.568497542209876	18.474032913015602	17.98247488779654	22.974994656977987
12	23.0989527676854	23.16734344945501	26.069673007052792	27.664030775806797
13	36.606112417183155	16.62748450523616	19.004060696730072	27.76234238085061
14	24.97542209873905	19.204958324428297	22.132934387689676	33.68668518914298
15	30.733062620217993	23.37251549476384	19.02543278478307	26.86898910023509
16	27.356272707843555	22.188501816627486	21.24385552468476	29.211369950844198
17	27.343449455011754	23.0562085915794	20.910450951057918	28.689891002350933
18	27.193844838640736	20.192348792477024	22.436418038042316	30.17738833083992
19	29.01047232314597	21.77388330839923	22.432143620431717	26.78350074802308
20	31.066467193844836	19.876041889292583	21.363539217781575	27.693951699080998
21	28.557384056422315	21.354990382560377	21.021585808933533	29.06603975208378
22	29.130156016242786	21.790980978841635	20.54285103654627	28.53601196836931
23	29.01902115836717	21.22248343663176	20.7651207522975	28.99337465270357
24	29.224193203675995	20.60269288309468	21.16691600769395	29.00619790553537
25	29.673007052789057	20.84206026928831	21.885018166274843	27.59991451164779
26	28.578756144475314	22.013250694592863	21.427655481940587	27.980337678991233
27	27.770891216071806	21.252404359905963	23.363966659542637	27.61273776447959
28	26.96730070527891	21.961957683265652	21.23530668946356	29.835434921991876
29	28.01025860226544	23.02201325069459	20.7736695875187	28.194058559521267
30	28.05727719598205	21.513143834152597	22.671511006625348	27.75806796324001
31	29.224193203675995	20.876255610173114	21.491771746099595	28.407779440051296
32	26.975849540500107	21.628553109638812	21.645650780081212	29.749946569779866
33	27.270784355631545	20.820688181235305	23.8469758495405	28.06155161359265
34	27.52724941226758	21.590083351143406	22.13720880530028	28.74545843128874
35	26.86471468262449	22.376576191493907	23.517845693524258	27.240863432357344
36	27.488779653772173	21.696943791408422	23.000641162641593	27.813635392177815
37	27.651207522974996	21.03013464415473	23.436631758922847	27.882026073947426
38	26.958751870057707	20.910450951057918	24.06924556529173	28.06155161359265
39	26.719384483864072	20.0	24.954050010686043	28.32656550544988
40	25.509724300064118	21.380636888223982	26.022654413336184	27.08698439837572
41	24.808719811925624	19.717888437700363	26.650993802094465	28.822397948279548
42	22.893780722376576	20.363325496901048	28.630049155802524	28.112844624919852
43	22.69288309467835	21.90639025432785	26.9373797820047	28.4633468689891
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	3.5
14	6.0
15	11.5
16	17.0
17	18.5
18	20.0
19	20.0
20	21.5
21	23.0
22	16.0
23	9.0
24	16.0
25	23.0
26	23.0
27	23.0
28	23.0
29	37.0
30	51.0
31	59.5
32	68.0
33	68.0
34	115.5
35	163.0
36	179.0
37	195.0
38	267.5
39	340.0
40	340.0
41	426.0
42	512.0
43	662.5
44	813.0
45	1145.0
46	1477.0
47	1477.0
48	1651.0
49	1825.0
50	2208.0
51	2591.0
52	2594.5
53	2598.0
54	2598.0
55	2573.5
56	2549.0
57	2465.5
58	2382.0
59	2234.5
60	2087.0
61	2087.0
62	1910.5
63	1734.0
64	1470.0
65	1206.0
66	1087.5
67	969.0
68	969.0
69	810.0
70	651.0
71	533.5
72	416.0
73	343.0
74	270.0
75	270.0
76	209.0
77	148.0
78	123.5
79	99.0
80	78.0
81	57.0
82	57.0
83	51.0
84	45.0
85	29.0
86	13.0
87	9.5
88	6.0
89	6.0
90	4.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23395.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.21201111348579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.68864685687227	64.76597563581961
2	5.090564697525749	7.351998290232956
3	1.6573931573339646	3.5905107929044666
4	1.012193678228957	2.9237016456507803
5	0.5268142535811531	1.9021158367172473
6	0.39067124422872024	1.6926693737978198
7	0.23085118977151653	1.1669160076939518
8	0.1894163608381674	1.0942509083137422
9	0.18349709956197466	1.192562513357555
>10	0.9944358944003788	12.588159863218637
>50	0.029596306380963656	1.2865997007907672
>100	0.005919261276192731	0.4445394315024578
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	104	0.4445394315024578	TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	72	0.30775806796324	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	63	0.269288309467835	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	63	0.269288309467835	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	52	0.2222697157512289	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	51	0.21799529814062832	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	50	0.21372088053002777	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	47	0.20089762769822614	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	43	0.1837999572558239	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	43	0.1837999572558239	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	42	0.17952553964522333	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	41	0.17525112203462279	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	40	0.1709767044240222	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	39	0.16670228681342167	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	35	0.14960461637101946	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	34	0.1453301987604189	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	34	0.1453301987604189	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	32	0.13678136353921777	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	32	0.13678136353921777	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	31	0.13250694592861723	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	31	0.13250694592861723	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	31	0.13250694592861723	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	31	0.13250694592861723	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	31	0.13250694592861723	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	30	0.12823252831801668	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	30	0.12823252831801668	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	29	0.12395811070741612	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	29	0.12395811070741612	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	29	0.12395811070741612	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	28	0.11968369309681556	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	28	0.11968369309681556	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	28	0.11968369309681556	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	28	0.11968369309681556	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	27	0.115409275486215	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	27	0.115409275486215	RNA PCR Primer, Index 6 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	26	0.11113485787561445	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	26	0.11113485787561445	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	26	0.11113485787561445	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	24	0.10258602265441334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.004274417610600555	0.0	0.0	0.0	0.0
18	0.004274417610600555	0.0	0.0	0.0	0.0
19	0.004274417610600555	0.0	0.0	0.0	0.0
20	0.004274417610600555	0.0	0.0	0.0	0.0
21	0.004274417610600555	0.0	0.0	0.0	0.0
22	0.004274417610600555	0.0	0.0	0.0	0.0
23	0.004274417610600555	0.0	0.0	0.004274417610600555	0.0
24	0.004274417610600555	0.0	0.0	0.00854883522120111	0.0
25	0.004274417610600555	0.0	0.0	0.00854883522120111	0.0
26	0.004274417610600555	0.0	0.0	0.012823252831801668	0.0
27	0.004274417610600555	0.0	0.0	0.025646505663603335	0.0
28	0.004274417610600555	0.0	0.0	0.09831160504381278	0.0
29	0.004274417610600555	0.0	0.0	0.18807437486642445	0.0
30	0.004274417610600555	0.0	0.0	0.3163069031844411	0.0
31	0.004274417610600555	0.0	0.0	0.6326138063688822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	35	2.290378E-5	31.714285	1
TACGGAG	25	0.005388136	29.6	30
GTATGCC	45	1.2698074E-4	24.666668	32
TGCCGTC	45	1.2698074E-4	24.666668	35
CCGTCTT	45	1.2698074E-4	24.666668	37
ATGCCGT	45	1.2698074E-4	24.666668	34
CGTATGC	45	1.2698074E-4	24.666668	31
GCCGTCT	45	1.2698074E-4	24.666668	36
CGCGGTA	40	0.001875412	23.125	22
TAATACG	40	0.001875412	23.125	27
AATACGG	40	0.001875412	23.125	28
GTAATAC	40	0.001875412	23.125	26
CCGCGGT	40	0.001875412	23.125	21
AGCCCAC	50	2.5939112E-4	22.2	7
ATCTCGT	50	2.5939112E-4	22.2	27
GCCCACG	50	2.5939112E-4	22.2	8
TATGCCG	50	2.5939112E-4	22.2	33
GAGCCCA	50	2.5939112E-4	22.2	6
CACGAGA	50	2.5939112E-4	22.2	11
TCTCGTA	50	2.5939112E-4	22.2	28
>>END_MODULE