FastQCFastQC Report
Fri 10 Feb 2017
ERR1632651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5502
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA190.34532897128316975No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT180.3271537622682661Illumina PCR Primer Index 10 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA160.2908033442384587No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA110.19992729916394036No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT100.1817520901490367No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC100.1817520901490367No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA90.16357688113413305No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA90.16357688113413305No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA90.16357688113413305No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT80.14540167211922936No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA70.1272264631043257No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT60.10905125408942204No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC60.10905125408942204No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA60.10905125408942204No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)