##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632651.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5502
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95528898582334	34.0	33.0	34.0	31.0	34.0
2	33.08687749909124	34.0	33.0	34.0	31.0	34.0
3	33.1601235914213	34.0	33.0	34.0	31.0	34.0
4	36.49291166848419	37.0	37.0	37.0	35.0	37.0
5	36.44165757906216	37.0	37.0	37.0	35.0	37.0
6	36.48727735368957	37.0	37.0	37.0	35.0	37.0
7	36.477826245001815	37.0	37.0	37.0	35.0	37.0
8	36.42911668484187	37.0	37.0	37.0	35.0	37.0
9	38.226826608505995	39.0	39.0	39.0	37.0	39.0
10	38.23209741912032	39.0	39.0	39.0	37.0	39.0
11	38.30443475099964	39.0	39.0	39.0	37.0	39.0
12	38.273173391494005	39.0	39.0	39.0	37.0	39.0
13	38.33460559796438	39.0	39.0	39.0	37.0	39.0
14	39.82260996001454	41.0	40.0	41.0	38.0	41.0
15	39.79589240276263	41.0	40.0	41.0	38.0	41.0
16	39.80388949472919	41.0	40.0	41.0	38.0	41.0
17	39.81352235550709	41.0	40.0	41.0	38.0	41.0
18	39.75972373682298	41.0	40.0	41.0	38.0	41.0
19	39.795165394402034	41.0	40.0	41.0	38.0	41.0
20	39.725009087604505	41.0	40.0	41.0	38.0	41.0
21	39.726826608505995	41.0	40.0	41.0	38.0	41.0
22	39.69720101781171	41.0	40.0	41.0	38.0	41.0
23	39.63104325699746	41.0	40.0	41.0	37.0	41.0
24	39.665212649945474	41.0	40.0	41.0	37.0	41.0
25	39.63794983642312	41.0	40.0	41.0	37.0	41.0
26	39.542166484914574	41.0	39.0	41.0	37.0	41.0
27	39.38949472918939	41.0	39.0	41.0	36.0	41.0
28	39.31733914940022	41.0	39.0	41.0	36.0	41.0
29	39.32479098509633	41.0	39.0	41.0	36.0	41.0
30	39.282079243911305	41.0	39.0	41.0	36.0	41.0
31	39.19956379498364	41.0	39.0	41.0	35.0	41.0
32	39.16921119592875	41.0	39.0	41.0	35.0	41.0
33	39.08705925118139	41.0	39.0	41.0	35.0	41.0
34	39.08360596146856	40.0	39.0	41.0	35.0	41.0
35	38.99200290803344	40.0	39.0	41.0	35.0	41.0
36	38.907124681933844	40.0	38.0	41.0	35.0	41.0
37	38.80461650308978	40.0	38.0	41.0	35.0	41.0
38	38.76335877862596	40.0	38.0	41.0	35.0	41.0
39	38.69011268629589	40.0	38.0	41.0	35.0	41.0
40	38.596510359869136	40.0	38.0	41.0	35.0	41.0
41	38.56961105052708	40.0	38.0	41.0	35.0	41.0
42	38.401308615049075	40.0	37.0	41.0	35.0	41.0
43	37.72846237731734	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	1.0
23	5.0
24	3.0
25	5.0
26	12.0
27	6.0
28	17.0
29	26.0
30	34.0
31	37.0
32	54.0
33	76.0
34	87.0
35	136.0
36	242.0
37	439.0
38	1127.0
39	3194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80225372591785	16.066884769174848	11.105052708106143	29.02580879680116
2	22.500908760450745	17.8117048346056	29.51653944020356	30.170846964740093
3	21.33769538349691	18.847691748455105	26.33587786259542	33.47873500545256
4	16.19411123227917	14.70374409305707	31.31588513267903	37.786259541984734
5	19.50199927299164	31.970192657215556	30.6979280261723	17.829880043620502
6	37.47728098873137	32.33369683751363	13.994910941475828	16.19411123227917
7	33.00617957106507	26.826608505997818	18.284260268993094	21.882951653944023
8	29.58924027626318	31.424936386768447	18.047982551799347	20.93784078516903
9	28.934932751726645	12.395492548164304	16.484914576517628	42.18466012359142
10	19.93820428934933	21.84660123591421	27.771719374772807	30.443475099963653
11	43.402399127589966	17.575427117411852	16.666666666666664	22.355507088331517
12	24.06397673573246	23.336968375136312	25.808796801163215	26.79025808796801
13	37.386404943656856	15.19447473645947	18.77499091239549	28.644129407488183
14	25.28171573973101	18.393311523082513	21.010541621228644	35.31443111595783
15	30.916030534351147	22.02835332606325	17.957106506724827	29.098509632860782
16	27.2446383133406	22.791712104689203	20.774263904034896	29.1893856779353
17	28.00799709196656	23.02798982188295	20.410759723736824	28.553253362413667
18	28.262450018175212	20.265358051617593	21.646673936750272	29.825517993456923
19	29.40748818611414	21.864776444929117	21.410396219556524	27.317339149400215
20	30.407124681933844	20.44711014176663	21.810250817884405	27.335514358415118
21	29.280261723009815	20.75608869501999	19.46564885496183	30.49800072700836
22	29.425663395129042	21.2831697564522	19.3020719738277	29.98909487459106
23	29.425663395129042	21.19229371137768	20.574336604870954	28.80770628862232
24	29.171210468920393	20.24718284260269	19.611050527081062	30.970556161395855
25	30.38894947291894	20.24718284260269	20.53798618684115	28.825881497637223
26	29.498364231188656	21.73754998182479	20.21083242457288	28.553253362413667
27	28.24427480916031	21.174118502362777	21.901126862958925	28.680479825517995
28	27.40821519447474	22.591784805525265	20.55616139585605	29.443838604143945
29	27.499091239549255	22.446383133406034	20.701563067975282	29.35296255906943
30	27.02653580516176	22.264631043256998	21.2831697564522	29.425663395129042
31	27.535441657579064	22.464558342420936	20.519810977826243	29.480189022173754
32	28.153398764085786	21.33769538349691	21.155943293347875	29.35296255906943
33	27.2264631043257	21.355870592511813	22.555434387495456	28.86223191566703
34	27.335514358415118	20.53798618684115	22.42820792439113	29.6982915303526
35	26.97201017811705	22.646310432569976	23.173391494002182	27.208287895310797
36	27.80806979280262	20.719738276990185	22.93711377680843	28.535078153398764
37	27.699018538713194	20.010905125408943	22.68266085059978	29.607415485278082
38	25.390766993820428	20.774263904034896	25.045438022537258	28.789531079607418
39	25.081788440567067	20.047255543438748	25.118138858596872	29.75281715739731
40	25.28171573973101	19.95637949836423	26.953834969102147	27.80806979280262
41	23.791348600508904	19.247546346782986	28.18974918211559	28.77135587059251
42	22.62813522355507	18.793166121410394	29.661941112322793	28.91675754271174
43	21.19229371137768	19.974554707379134	29.02580879680116	29.80734278444202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.5
11	1.0
12	1.0
13	1.5
14	2.0
15	1.0
16	0.0
17	1.5
18	3.0
19	3.0
20	4.5
21	6.0
22	6.0
23	6.0
24	5.5
25	5.0
26	5.0
27	8.0
28	11.0
29	12.0
30	13.0
31	17.0
32	21.0
33	21.0
34	27.5
35	34.0
36	41.0
37	48.0
38	70.0
39	92.0
40	92.0
41	104.0
42	116.0
43	176.0
44	236.0
45	260.0
46	284.0
47	284.0
48	333.5
49	383.0
50	434.5
51	486.0
52	486.0
53	486.0
54	486.0
55	542.5
56	599.0
57	567.0
58	535.0
59	531.0
60	527.0
61	527.0
62	491.0
63	455.0
64	396.0
65	337.0
66	298.0
67	259.0
68	259.0
69	228.5
70	198.0
71	169.5
72	141.0
73	119.5
74	98.0
75	98.0
76	76.5
77	55.0
78	42.0
79	29.0
80	23.0
81	17.0
82	17.0
83	12.0
84	7.0
85	7.5
86	8.0
87	4.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5502.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.33042529989095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.02487562189054	86.78662304616503
2	3.2238805970149254	5.888767720828789
3	0.855721393034826	2.3446019629225736
4	0.4975124378109453	1.817520901490367
5	0.11940298507462686	0.5452562704471101
6	0.05970149253731343	0.3271537622682661
7	0.01990049751243781	0.1272264631043257
8	0.01990049751243781	0.14540167211922936
9	0.05970149253731343	0.49073064340239914
>10	0.11940298507462686	1.5267175572519083
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	19	0.34532897128316975	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	18	0.3271537622682661	Illumina PCR Primer Index 10 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	16	0.2908033442384587	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	11	0.19992729916394036	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	10	0.1817520901490367	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	10	0.1817520901490367	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	9	0.16357688113413305	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	9	0.16357688113413305	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	9	0.16357688113413305	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	8	0.14540167211922936	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	7	0.1272264631043257	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	6	0.10905125408942204	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	6	0.10905125408942204	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	6	0.10905125408942204	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.01817520901490367	0.0
22	0.0	0.0	0.0	0.01817520901490367	0.0
23	0.0	0.0	0.0	0.01817520901490367	0.0
24	0.0	0.0	0.0	0.01817520901490367	0.0
25	0.0	0.0	0.0	0.01817520901490367	0.0
26	0.0	0.0	0.0	0.01817520901490367	0.0
27	0.0	0.0	0.0	0.07270083605961468	0.0
28	0.0	0.0	0.0	0.1999272991639404	0.0
29	0.0	0.0	0.0	0.3998545983278808	0.0
30	0.0	0.0	0.0	0.7997091966557616	0.0
31	0.0	0.0	0.0	1.3994910941475827	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE