FastQCFastQC Report
Fri 10 Feb 2017
ERR1632650.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632650.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347272
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8210.23641410767352394No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6820.19638784583842062No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6160.17738257043470249No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5140.14801078117441083No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4990.14369140040083853No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC4840.13937201962726625No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4840.13937201962726625No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4610.13274896910778872No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4550.1310212167983598No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4440.12785367089774008No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG4340.12497408371535856No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG4330.12468612499712041No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4170.12007878550530995No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3910.11259185883111798No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC3880.11172798267640352No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG3800.10942431293049829No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3800.10942431293049829No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3680.10596880831164045No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3630.10452901472044968No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3630.10452901472044968No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA3560.1025133036927826No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3560.1025133036927826No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3480.10020963394687737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT554.984031E-1033.63636436
TATGGGT250.005491127329.64
TTTATGA250.005491127329.62
CTTCTAG250.005491127329.61
ATACGGC453.997675E-628.77777729
GGTATCA3850.027.389611
TCCTGTA358.8568823E-426.4285722
TCTAGCA358.8568823E-426.4285723
AACTCCG3750.023.1866655
CTCGTAT802.7119313E-823.12500229
ATCCCAT400.001928488523.1250026
CGGACCT400.001928488523.12500234
TAACTCC3800.022.8815784
TAATACG3500.022.72857327
GCGGTAA3800.022.39473723
TAAGACT502.696843E-422.24
GGTAATA3950.022.01265725
CGTATGC855.1544703E-821.76470631
GTATGCC855.1544703E-821.76470632
ATGCCGT855.1544703E-821.76470634
Produced by FastQC (version 0.11.5)