FastQCFastQC Report
Fri 10 Feb 2017
ERR1632649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320923
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4690.14614097462631223No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4290.13367692561767153No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3930.1224592815098949No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3350.10438641044736587No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3260.10158199942042173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3050.027.2950821
CTTCTAT451.3203101E-424.6666681
GTATCAA4250.019.5882362
GTAACGT902.1423948E-618.526
ATACACA1103.8258804E-818.537
CTTATAC3300.017.37878637
GCGGTTT1005.851278E-616.6528
TAACGTC1005.851278E-616.6527
TCACTAA1606.220944E-1016.187533
GGCGGTT1201.9248437E-615.41666727
GTCACTA1701.4697434E-915.23529432
GGTAACG1101.445315E-515.13636425
TCTTATA5100.014.87254937
GTATTAG750.0040958414.81
GTCGTCC750.0040958414.88
AATACTG750.0040958414.85
ACTATCG1001.0894681E-414.79999911
CCTGTAC1001.0894681E-414.7999993
GTACTGG1252.9425064E-614.7999991
TATACAC1252.9425064E-614.79999936
Produced by FastQC (version 0.11.5)