##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632649.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320923
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05855298623034	34.0	33.0	34.0	31.0	34.0
2	33.20306117043652	34.0	33.0	34.0	31.0	34.0
3	33.28139460244358	34.0	34.0	34.0	31.0	34.0
4	36.556865665595794	37.0	37.0	37.0	35.0	37.0
5	36.5248455236926	37.0	37.0	37.0	35.0	37.0
6	36.563618064146226	37.0	37.0	37.0	35.0	37.0
7	36.558694764787816	37.0	37.0	37.0	35.0	37.0
8	36.5334955737046	37.0	37.0	37.0	35.0	37.0
9	38.324576300234014	39.0	39.0	39.0	37.0	39.0
10	38.34846676617133	39.0	39.0	39.0	37.0	39.0
11	38.419340464846705	39.0	39.0	39.0	37.0	39.0
12	38.38735771509054	39.0	39.0	39.0	37.0	39.0
13	38.41635532510914	39.0	39.0	39.0	37.0	39.0
14	39.99070181943955	41.0	40.0	41.0	38.0	41.0
15	39.97861169190117	41.0	40.0	41.0	38.0	41.0
16	39.95052707347245	41.0	40.0	41.0	38.0	41.0
17	39.942145000514145	41.0	40.0	41.0	38.0	41.0
18	39.93061575518115	41.0	40.0	41.0	38.0	41.0
19	39.93168454738364	41.0	40.0	41.0	38.0	41.0
20	39.90378688969005	41.0	40.0	41.0	38.0	41.0
21	39.874670871205865	41.0	40.0	41.0	38.0	41.0
22	39.844676137266575	41.0	40.0	41.0	38.0	41.0
23	39.804825456573695	41.0	40.0	41.0	38.0	41.0
24	39.786955126307554	41.0	40.0	41.0	38.0	41.0
25	39.7430193535521	41.0	40.0	41.0	38.0	41.0
26	39.65946971703493	41.0	40.0	41.0	37.0	41.0
27	39.585679430891524	41.0	40.0	41.0	37.0	41.0
28	39.53012716446001	41.0	40.0	41.0	37.0	41.0
29	39.48026162038869	41.0	40.0	41.0	37.0	41.0
30	39.43657824462566	41.0	39.0	41.0	37.0	41.0
31	39.3760590546642	41.0	39.0	41.0	36.0	41.0
32	39.326149886421355	41.0	39.0	41.0	36.0	41.0
33	39.247906818769614	41.0	39.0	41.0	36.0	41.0
34	39.201824113572414	41.0	39.0	41.0	35.0	41.0
35	39.129791258339225	41.0	39.0	41.0	35.0	41.0
36	39.07585931827884	41.0	39.0	41.0	35.0	41.0
37	39.018356428177476	41.0	39.0	41.0	35.0	41.0
38	38.936523714411244	40.0	39.0	41.0	35.0	41.0
39	38.85275595703642	40.0	38.0	41.0	35.0	41.0
40	38.77126912063018	40.0	38.0	41.0	35.0	41.0
41	38.71313679605388	40.0	38.0	41.0	35.0	41.0
42	38.64686544747494	40.0	38.0	41.0	35.0	41.0
43	37.89964570940693	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	3.0
19	3.0
20	14.0
21	29.0
22	57.0
23	118.0
24	178.0
25	301.0
26	393.0
27	482.0
28	729.0
29	981.0
30	1418.0
31	1813.0
32	2382.0
33	3256.0
34	4940.0
35	8011.0
36	12761.0
37	24079.0
38	62266.0
39	196706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79835661513821	15.897894510521215	11.871383478279837	28.43236539606074
2	21.62886424469421	18.56333139101903	31.317481140335847	28.490323223950913
3	21.842311083967182	18.79640910748061	27.531526253961232	31.82975355459098
4	16.670353947831725	14.650866407206713	31.545261635968753	37.13351800899281
5	18.40161035513192	31.80513705779891	32.060961663701256	17.73229092336791
6	37.764510490055244	31.38042458782948	14.174428133851421	16.68063678826385
7	32.43425993151005	26.151444427479486	18.929151229422633	22.485144411587825
8	29.05494464404234	30.08540989583171	18.692645899483676	22.16699956064227
9	28.412111316421694	12.53073167083693	16.715536125488047	42.34162088725333
10	19.75894529217289	22.941640206529293	29.401445206482553	27.897969294815265
11	40.836586969459965	18.397247937978893	18.138930522274812	22.62723457028633
12	24.150964561592655	22.22526898975767	25.991592998943673	27.632173449706006
13	35.525967288103374	16.750747063937453	19.849309647485534	27.873976000473633
14	25.130327212446602	18.340848116214794	21.871601599137488	34.65722307220112
15	29.974479859654807	23.31587327801373	18.92011479389137	27.789532068440092
16	27.53931628459163	22.494492448344307	21.13435310027639	28.83183816678767
17	27.822873399538206	22.48389800668696	21.20010095879697	28.49312763497786
18	28.002044104037417	20.897224567887	22.23991424734282	28.860817080732758
19	28.962399080153183	21.39890254048479	21.985024445116117	27.65367393424591
20	29.819302449497233	20.68845174699227	21.76814999236577	27.72409581114473
21	28.537375008958538	21.359952387332786	21.278936068776623	28.823736534932053
22	29.2428401828476	21.745403102925	20.53576714663642	28.475989567590982
23	29.017552497016418	21.333466283189427	21.2901537128844	28.358827506909755
24	28.86798390891273	21.03121309472989	21.25244996463326	28.848353031724123
25	29.191737581912168	20.91311623037302	21.606428956478656	28.288717231236156
26	28.891042399578716	21.721721409808584	21.367119215512755	28.020116975099945
27	28.232940611922487	21.330350270937267	22.164195149615328	28.27251396752492
28	27.654297136696343	21.922392598847697	21.246217940128943	29.17709232432702
29	28.3226817647847	22.36143872517707	21.269899633245355	28.045979876792877
30	28.03600863758596	21.429751061781175	22.07570040165398	28.458539898978884
31	28.535817002832452	21.40357655886303	21.487085687220922	28.573520751083592
32	27.309042979156995	21.431309067907257	21.76441077766318	29.49523717527257
33	27.448951929278987	21.109113401033895	22.83756539730714	28.60436927237998
34	27.604752541886995	20.957052003128478	22.579247981603064	28.858947473381463
35	27.107748587667444	21.848543108471503	23.266017081979165	27.777691221881884
36	27.30000654362573	20.778192899854485	23.701323993605943	28.220476562913845
37	27.26074478924851	20.384328951181434	23.66393184658002	28.690994412990033
38	26.43095072649826	20.216375890790005	24.92529360625446	28.42737977645728
39	25.966041698475955	19.589434225655374	25.78468978540024	28.659834290468428
40	25.602403068648865	20.20328863933093	26.63317992166345	27.56112837035675
41	24.367839014343005	19.72061834147132	27.333659475949055	28.577883168236617
42	23.22800173250281	20.222607915294322	28.382509200026174	28.166881152176693
43	22.871218329630473	20.216375890790005	28.040371054738987	28.87203472484054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	4.0
4	7.0
5	7.0
6	9.5
7	12.0
8	11.0
9	10.0
10	14.5
11	19.0
12	19.0
13	43.5
14	68.0
15	120.0
16	172.0
17	163.5
18	155.0
19	155.0
20	220.0
21	285.0
22	265.0
23	245.0
24	299.5
25	354.0
26	354.0
27	479.0
28	604.0
29	789.0
30	974.0
31	1317.5
32	1661.0
33	1661.0
34	2288.5
35	2916.0
36	3459.0
37	4002.0
38	5107.5
39	6213.0
40	6213.0
41	7401.0
42	8589.0
43	10427.5
44	12266.0
45	15653.5
46	19041.0
47	19041.0
48	21388.5
49	23736.0
50	26814.5
51	29893.0
52	30939.0
53	31985.0
54	31985.0
55	31592.5
56	31200.0
57	30940.5
58	30681.0
59	29632.5
60	28584.0
61	28584.0
62	26598.5
63	24613.0
64	21664.5
65	18716.0
66	16607.0
67	14498.0
68	14498.0
69	12553.0
70	10608.0
71	8945.5
72	7283.0
73	5918.0
74	4553.0
75	4553.0
76	3719.0
77	2885.0
78	2371.5
79	1858.0
80	1496.5
81	1135.0
82	1135.0
83	853.5
84	572.0
85	441.5
86	311.0
87	222.5
88	134.0
89	134.0
90	98.5
91	63.0
92	39.0
93	15.0
94	9.0
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	320923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.41947829004788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.55698157037737	63.06555919417406
2	6.175290557762887	8.697214787342425
3	1.6027131792566227	3.38586677795506
4	0.7262216235391966	2.0456059141032714
5	0.4034527309831451	1.4205465415264054
6	0.27790738084952704	1.1742055663426398
7	0.1932956463557137	0.9528245000474866
8	0.14399984785570505	0.8112315327876015
9	0.10374952570228758	0.6575388725515504
>10	0.7104665110320755	9.618911387145674
>50	0.06825062614212193	3.370251134688415
>100	0.037670800143368675	4.800243791335492
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	469	0.14614097462631223	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	429	0.13367692561767153	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	393	0.1224592815098949	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	335	0.10438641044736587	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	326	0.10158199942042173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	3.1160122521601755E-4	0.0
15	0.0	0.0	0.0	3.1160122521601755E-4	0.0
16	0.0	0.0	0.0	6.232024504320351E-4	0.0
17	0.0	0.0	0.0	0.0012464049008640702	0.0
18	0.0	3.1160122521601755E-4	0.0	0.0015580061260800878	0.0
19	0.0	3.1160122521601755E-4	0.0	0.0015580061260800878	0.0
20	0.0	3.1160122521601755E-4	0.0	0.0015580061260800878	0.0
21	0.0	3.1160122521601755E-4	0.0	0.0024928098017281404	0.0
22	0.0	3.1160122521601755E-4	0.0	0.003739214702592211	0.0
23	0.0	3.1160122521601755E-4	0.0	0.005297220828672298	0.0
24	0.0	3.1160122521601755E-4	0.0	0.007166828179968404	0.0
25	0.0	3.1160122521601755E-4	0.0	0.008413233080832474	0.0
26	0.0	3.1160122521601755E-4	0.0	0.01277565023385672	0.0
27	0.0	3.1160122521601755E-4	0.0	0.03801534947635414	0.0
28	0.0	3.1160122521601755E-4	0.0	0.12277088273511091	0.0
29	0.0	3.1160122521601755E-4	0.0	0.24896937894759802	0.0
30	0.0	3.1160122521601755E-4	0.0	0.3982263658260704	0.0
31	0.0	3.1160122521601755E-4	0.0	0.8924259090186742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	305	0.0	27.295082	1
CTTCTAT	45	1.3203101E-4	24.666668	1
GTATCAA	425	0.0	19.588236	2
GTAACGT	90	2.1423948E-6	18.5	26
ATACACA	110	3.8258804E-8	18.5	37
CTTATAC	330	0.0	17.378786	37
GCGGTTT	100	5.851278E-6	16.65	28
TAACGTC	100	5.851278E-6	16.65	27
TCACTAA	160	6.220944E-10	16.1875	33
GGCGGTT	120	1.9248437E-6	15.416667	27
GTCACTA	170	1.4697434E-9	15.235294	32
GGTAACG	110	1.445315E-5	15.136364	25
TCTTATA	510	0.0	14.872549	37
GTATTAG	75	0.00409584	14.8	1
GTCGTCC	75	0.00409584	14.8	8
AATACTG	75	0.00409584	14.8	5
ACTATCG	100	1.0894681E-4	14.799999	11
CCTGTAC	100	1.0894681E-4	14.799999	3
GTACTGG	125	2.9425064E-6	14.799999	1
TATACAC	125	2.9425064E-6	14.799999	36
>>END_MODULE