FastQCFastQC Report
Fri 10 Feb 2017
ERR1632648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65516
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1420.21674094877587152No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1360.20758288051773616No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1260.19231943342084376No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1250.19079308871115452No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1140.17400329690457295No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1070.16331888393674826No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1070.16331888393674826No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT1060.16179253922705905RNA PCR Primer, Index 33 (95% over 22bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT930.14195005800109897No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT880.1343183344526528No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC850.12973930032358508No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT840.12821295561389584No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA800.1221075767751389No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA800.1221075767751389No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA790.12058123206544966No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA770.11752854264607118No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA750.11447585322669272No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA710.10837047438793576No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA690.1053177849685573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACA200.001829294337.04
ATTGCAC200.001829294337.03
GCGGTAA352.3543485E-531.71428523
AACTCCG352.3543485E-531.7142855
TATCGCG303.5628703E-430.8333347
CTAGACA250.00545905229.64
TCTTACC250.00545905229.62
GCACAAT250.00545905229.66
AGCCGCG405.8563404E-527.75000219
CTCCGTG405.8563404E-527.7500027
CGTGCCA405.8563404E-527.75000210
CTAACTC405.8563404E-527.7500023
TCCGTGC405.8563404E-527.7500028
GCTAACT405.8563404E-527.7500022
TAACTCC405.8563404E-527.7500024
GGCTAAC405.8563404E-527.7500021
GTGCCAG405.8563404E-527.75000211
GCGCCTT358.7800436E-426.4285713
CGCCTTT358.7800436E-426.4285714
ATCGCGC358.7800436E-426.428578
Produced by FastQC (version 0.11.5)