##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632648.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65516
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06519018255083	34.0	33.0	34.0	31.0	34.0
2	33.20179803406801	34.0	33.0	34.0	31.0	34.0
3	33.261356004640085	34.0	34.0	34.0	31.0	34.0
4	36.546080346785516	37.0	37.0	37.0	35.0	37.0
5	36.51540081812077	37.0	37.0	37.0	35.0	37.0
6	36.560824836681114	37.0	37.0	37.0	35.0	37.0
7	36.56596861835277	37.0	37.0	37.0	35.0	37.0
8	36.53005372733378	37.0	37.0	37.0	35.0	37.0
9	38.32607302033091	39.0	39.0	39.0	37.0	39.0
10	38.35418828988339	39.0	39.0	39.0	37.0	39.0
11	38.43058184260333	39.0	39.0	39.0	37.0	39.0
12	38.40179498137859	39.0	39.0	39.0	37.0	39.0
13	38.43529824775627	39.0	39.0	39.0	37.0	39.0
14	40.00210635569937	41.0	40.0	41.0	38.0	41.0
15	39.99171194822639	41.0	40.0	41.0	38.0	41.0
16	39.955110202088036	41.0	40.0	41.0	38.0	41.0
17	39.95132486720801	41.0	40.0	41.0	38.0	41.0
18	39.936671957994996	41.0	40.0	41.0	38.0	41.0
19	39.938076195127906	41.0	40.0	41.0	38.0	41.0
20	39.923667501068444	41.0	40.0	41.0	38.0	41.0
21	39.89071371878625	41.0	40.0	41.0	38.0	41.0
22	39.85397460162403	41.0	40.0	41.0	38.0	41.0
23	39.81261065999145	41.0	40.0	41.0	38.0	41.0
24	39.77565785456988	41.0	40.0	41.0	38.0	41.0
25	39.74723731607546	41.0	40.0	41.0	38.0	41.0
26	39.66530313205934	41.0	40.0	41.0	37.0	41.0
27	39.59292386592588	41.0	40.0	41.0	37.0	41.0
28	39.54145552231516	41.0	40.0	41.0	37.0	41.0
29	39.50891385310459	41.0	40.0	41.0	37.0	41.0
30	39.45718603089322	41.0	40.0	41.0	36.0	41.0
31	39.39170584284755	41.0	39.0	41.0	36.0	41.0
32	39.3384211490323	41.0	39.0	41.0	36.0	41.0
33	39.273658343000186	41.0	39.0	41.0	36.0	41.0
34	39.230478051163075	41.0	39.0	41.0	35.0	41.0
35	39.15721350509799	41.0	39.0	41.0	35.0	41.0
36	39.10495146223823	41.0	39.0	41.0	35.0	41.0
37	39.03957811832224	41.0	39.0	41.0	35.0	41.0
38	38.964558275841014	41.0	39.0	41.0	35.0	41.0
39	38.862476341657	41.0	39.0	41.0	35.0	41.0
40	38.78333536845961	40.0	38.0	41.0	35.0	41.0
41	38.74326881983027	40.0	38.0	41.0	35.0	41.0
42	38.66678368642774	40.0	38.0	41.0	35.0	41.0
43	37.917302643629036	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	5.0
20	9.0
21	12.0
22	20.0
23	25.0
24	35.0
25	58.0
26	77.0
27	87.0
28	169.0
29	204.0
30	294.0
31	374.0
32	484.0
33	675.0
34	971.0
35	1641.0
36	2523.0
37	4709.0
38	12228.0
39	40913.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.60919470053117	15.887722083155259	12.226021124610781	28.277062091702792
2	21.196348983454424	18.78167165272605	31.505281152695524	28.516698211123998
3	21.751938457781307	19.279260028084742	27.668050552536783	31.300750961597167
4	16.576103547225106	15.010073875083949	32.05323890347396	36.36058367421698
5	17.92081323646132	31.981500702118566	32.92172904328714	17.175957018132976
6	36.32700409060382	32.208926063862265	14.981073325599853	16.482996519934062
7	31.465596190243605	26.736980279626348	19.558581109957874	22.238842420172173
8	28.67696440564137	29.74387935771415	19.557054765248182	22.0221014713963
9	28.281641125831857	12.36949752732157	17.081323646132244	42.26753770071433
10	19.56773917821601	22.910434092435437	29.68587825874596	27.835948470602602
11	40.220709445021065	18.58019415104707	18.20166066304414	22.997435740887724
12	23.55913059405336	22.82343244398315	25.967702545942977	27.649734416020515
13	35.60656938763051	16.753159533549056	20.288173881189326	27.352097197631114
14	25.166371573356127	18.972464741437207	22.16557787410709	33.695585811099576
15	30.265889248427865	22.689114109530497	19.40289394956957	27.642102692472072
16	27.14604066182307	22.873801819402896	21.150558642163748	28.829598876610298
17	27.912265706087062	22.960803467855182	21.074241406679285	28.052689419378474
18	27.420782709567128	20.749129983515477	22.654008181207644	29.17607912570975
19	28.611331583124734	21.96104768300873	21.993100921912205	27.434519811954335
20	29.99419989010318	20.639233164417853	21.90457292875023	27.461994016728735
21	28.350326637767875	21.77483362842664	21.18719091519629	28.687648818609198
22	29.678246535197513	21.886256792233958	20.410281457964466	28.025215214604067
23	29.150131265645037	21.5260394407473	21.16124305513157	28.162586238476095
24	28.666279992673545	21.329140973197386	21.32150924964894	28.683069784480125
25	29.330239941388363	21.54130288784419	21.709200805910005	27.419256364857443
26	28.754807985835523	22.212894560107454	21.356615177971793	27.67568227608523
27	28.49685572989804	21.210086085841624	22.80358996275719	27.489468221503145
28	27.326149337566395	22.125892911655168	21.51993406190854	29.02802368886989
29	28.20227120092802	22.356370962818243	21.391721106294646	28.049636729959094
30	27.70926185969839	21.455827584101595	22.6036388057879	28.231271750412112
31	28.406801392026377	21.806886867330117	21.588619573844557	28.197692166798948
32	26.724769521948836	21.622199157457718	21.78399169668478	29.869039623908662
33	27.002564259112276	21.576408816167042	22.98522498321021	28.43580194151047
34	27.6665242078271	21.214665119970693	22.38079247817327	28.738018194028943
35	26.70034800659381	22.489162952561205	23.710238720312596	27.10025032053239
36	27.16130410891996	21.835887416814213	23.432443983149152	27.57036449111667
37	27.452835948470604	21.02234568654985	23.872031259539657	27.65278710543989
38	26.5599242933024	20.814762806032114	24.46272666218939	28.162586238476095
39	26.11728432749252	20.256120642285854	25.410586726906402	28.21600830331522
40	25.67311801697295	20.90634348861347	26.375236583430002	27.045301910983575
41	24.470358385737832	19.837902191831002	27.184199279565295	28.507540142865867
42	23.01727822211368	20.689602539837598	27.951950668538984	28.34116856950974
43	22.847853959338178	20.984187068807618	27.590206972342635	28.57775199951157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	2.0
9	3.0
10	3.5
11	4.0
12	4.0
13	8.0
14	12.0
15	31.0
16	50.0
17	41.5
18	33.0
19	33.0
20	44.0
21	55.0
22	52.0
23	49.0
24	60.0
25	71.0
26	71.0
27	84.5
28	98.0
29	138.5
30	179.0
31	246.5
32	314.0
33	314.0
34	430.0
35	546.0
36	667.5
37	789.0
38	1022.0
39	1255.0
40	1255.0
41	1492.0
42	1729.0
43	2124.0
44	2519.0
45	3302.5
46	4086.0
47	4086.0
48	4604.5
49	5123.0
50	5894.0
51	6665.0
52	7055.0
53	7445.0
54	7445.0
55	7069.5
56	6694.0
57	6487.0
58	6280.0
59	5866.5
60	5453.0
61	5453.0
62	5202.0
63	4951.0
64	4154.5
65	3358.0
66	2943.5
67	2529.0
68	2529.0
69	2169.0
70	1809.0
71	1567.5
72	1326.0
73	1097.5
74	869.0
75	869.0
76	700.0
77	531.0
78	413.5
79	296.0
80	231.5
81	167.0
82	167.0
83	140.5
84	114.0
85	88.0
86	62.0
87	45.0
88	28.0
89	28.0
90	20.0
91	12.0
92	9.5
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	65516.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.20904817143904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.40173786472002	61.182001343183344
2	5.943585558961692	8.226997985224983
3	1.8547515603290474	3.850967702545943
4	0.9769975519925898	2.7046828255693267
5	0.6329532673179984	2.190304658404054
6	0.42343911959949715	1.7583491055620002
7	0.27567651015592265	1.3355516209780818
8	0.2668548618309331	1.4775016789791806
9	0.14335178528107978	0.8929116551682033
>10	1.0144895573737953	12.595396544355578
>50	0.04851906578744238	2.3154649245985715
>100	0.01764329664997905	1.4698699554307344
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	142	0.21674094877587152	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	136	0.20758288051773616	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	126	0.19231943342084376	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	125	0.19079308871115452	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	114	0.17400329690457295	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	107	0.16331888393674826	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	107	0.16331888393674826	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	106	0.16179253922705905	RNA PCR Primer, Index 33 (95% over 22bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	93	0.14195005800109897	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	88	0.1343183344526528	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	85	0.12973930032358508	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	84	0.12821295561389584	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	80	0.1221075767751389	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	80	0.1221075767751389	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	79	0.12058123206544966	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	77	0.11752854264607118	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	75	0.11447585322669272	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	71	0.10837047438793576	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	69	0.1053177849685573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0015263447096892362
16	0.0	0.0	0.0	0.0	0.0015263447096892362
17	0.0	0.0	0.0	0.0	0.0015263447096892362
18	0.0	0.0	0.0	0.0	0.0015263447096892362
19	0.0015263447096892362	0.0	0.0	0.0	0.0015263447096892362
20	0.0015263447096892362	0.0	0.0	0.0	0.0015263447096892362
21	0.0015263447096892362	0.0	0.0	0.0	0.0015263447096892362
22	0.0015263447096892362	0.0	0.0	0.0015263447096892362	0.0015263447096892362
23	0.0015263447096892362	0.0	0.0	0.0015263447096892362	0.0015263447096892362
24	0.0015263447096892362	0.0	0.0	0.004579034129067709	0.0015263447096892362
25	0.0015263447096892362	0.0	0.0	0.004579034129067709	0.0015263447096892362
26	0.0015263447096892362	0.0	0.0	0.007631723548446181	0.0015263447096892362
27	0.0015263447096892362	0.0	0.0	0.018316136516270835	0.0015263447096892362
28	0.0015263447096892362	0.0	0.0	0.08394895903290799	0.0015263447096892362
29	0.0015263447096892362	0.0	0.0	0.1862140545820868	0.0015263447096892362
30	0.0015263447096892362	0.0	0.0	0.3251114231638073	0.0015263447096892362
31	0.0015263447096892362	0.0	0.0	0.7295927712314549	0.0015263447096892362
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCACA	20	0.0018292943	37.0	4
ATTGCAC	20	0.0018292943	37.0	3
GCGGTAA	35	2.3543485E-5	31.714285	23
AACTCCG	35	2.3543485E-5	31.714285	5
TATCGCG	30	3.5628703E-4	30.833334	7
CTAGACA	25	0.005459052	29.6	4
TCTTACC	25	0.005459052	29.6	2
GCACAAT	25	0.005459052	29.6	6
AGCCGCG	40	5.8563404E-5	27.750002	19
CTCCGTG	40	5.8563404E-5	27.750002	7
CGTGCCA	40	5.8563404E-5	27.750002	10
CTAACTC	40	5.8563404E-5	27.750002	3
TCCGTGC	40	5.8563404E-5	27.750002	8
GCTAACT	40	5.8563404E-5	27.750002	2
TAACTCC	40	5.8563404E-5	27.750002	4
GGCTAAC	40	5.8563404E-5	27.750002	1
GTGCCAG	40	5.8563404E-5	27.750002	11
GCGCCTT	35	8.7800436E-4	26.42857	13
CGCCTTT	35	8.7800436E-4	26.42857	14
ATCGCGC	35	8.7800436E-4	26.42857	8
>>END_MODULE