FastQCFastQC Report
Fri 10 Feb 2017
ERR1632647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117581
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3370.2866109320383395No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2680.2279279815616469No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1980.1683945535418137No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1690.14373070479074No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1650.14032879461817813No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1620.1377773619887567No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1530.13012306410049243No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1430.12161828866908769No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT1420.12076781112594721TruSeq Adapter, Index 5 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1360.1156649458671044No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1350.11481446832396391No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1320.11226303569454249No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1280.1088611255219806No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1270.10801064797884012No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1210.10290778271999727No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1190.10120682763371633No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1190.10120682763371633No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1180.10035635009057586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG200.001835222737.01
ATCTAAG303.580026E-430.83333432
GGTATCA1150.030.5652141
GTAATGA250.005476541429.5999983
GTCAGCT250.005476541429.59999816
CATCTAA358.821916E-426.42857231
TCTAAGT358.821916E-426.42857233
ACATCTA400.001920947223.12500230
GCGGTAA903.739842E-922.6111123
CATAGGG450.00380571720.5555552
TGTGTCA450.00380571720.55555523
GGTAATA1001.2603778E-820.34999825
CGGTAAT1001.2603778E-820.34999824
CGCGGTA1052.2082531E-819.3809522
CTTATAC1052.2082531E-819.3809537
GCCGCTA701.2074245E-418.526
TTAAGTC609.1697474E-418.536
TGATTCA1103.7647624E-818.510
ATCCAGG500.006998292618.4999981
GTATCAA1950.018.0256422
Produced by FastQC (version 0.11.5)