FastQCFastQC Report
Fri 10 Feb 2017
ERR1632645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79727
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1470.18437919399952338No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1140.14298794636697731No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1110.13922510567310947No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT1020.12793658359150603TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1020.12793658359150603No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT970.12166518243505965No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT970.12166518243505965No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC930.11664806150990255No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA890.11163094058474544No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT800.10034241850314198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA251.2233463E-436.99999637
AGCGTAC303.5697664E-430.8333341
GTACACG303.5697664E-430.8333344
ATCAGAA303.5697664E-430.8333342
GCGTACA303.5697664E-430.8333342
AACCGTT250.005466085429.5999987
ACGGGGT250.005466085429.59999815
CCGAGGT250.005466085429.59999832
AGGATTG250.005466085429.5999985
GGTATCA652.901288E-928.4615381
GTATTAG358.796874E-426.4285721
GGTGGAT358.796874E-426.42857210
TCAGAAG358.796874E-426.4285723
CGGTGGA358.796874E-426.4285729
CATCAGA358.796874E-426.4285721
TACACGG358.796874E-426.4285725
TGCTCCG451.30818E-424.66666828
ACGGTGG451.30818E-424.6666688
AGAAGAG400.001915546623.1255
AGGCGAT400.001915546623.12531
Produced by FastQC (version 0.11.5)