FastQCFastQC Report
Fri 10 Feb 2017
ERR1632644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181911
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4300.23637932835287584No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3310.18195711089488817No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2500.1374298420656255No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2310.12698517406863796No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2110.11599078670338792No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2040.1121427511255504No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1960.1077449961794504No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1930.10609583807466287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1500.029.61
GTGTTAC250.0054843429.5999981
CACTATA250.0054843429.5999982
GTAGTTC250.0054843429.5999986
TAGTAGT250.0054843429.5999984
GCGGTAA1400.026.4285723
CTTATAC1600.025.43750237
TAATACG1550.022.6774227
GGTAATA1650.022.42424225
GTAATAC1650.022.42424226
CGGTAAT1650.022.42424224
AACTCCG1600.021.9687525
AATACGG1600.021.96875228
ATACGGA1450.021.68965529
TAAAGAC603.7041373E-521.58333437
CTAACTC1650.021.303033
CGCGGTA1750.021.14285722
TAACTCC1700.020.6764724
GGTACTT450.00381362420.5555572
AGCCGCG1800.020.55555719
Produced by FastQC (version 0.11.5)