##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632644.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181911
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.049067950811114	34.0	33.0	34.0	31.0	34.0
2	33.194237841581874	34.0	33.0	34.0	31.0	34.0
3	33.26951091467806	34.0	34.0	34.0	31.0	34.0
4	36.55284727146792	37.0	37.0	37.0	35.0	37.0
5	36.51371274964131	37.0	37.0	37.0	35.0	37.0
6	36.55952636179231	37.0	37.0	37.0	35.0	37.0
7	36.559251502108175	37.0	37.0	37.0	35.0	37.0
8	36.5359269093128	37.0	37.0	37.0	35.0	37.0
9	38.326549796328976	39.0	39.0	39.0	37.0	39.0
10	38.35021521513268	39.0	39.0	39.0	37.0	39.0
11	38.419617285375814	39.0	39.0	39.0	37.0	39.0
12	38.3792513921643	39.0	39.0	39.0	37.0	39.0
13	38.41376277410382	39.0	39.0	39.0	37.0	39.0
14	39.96656057082859	41.0	40.0	41.0	38.0	41.0
15	39.957973954296335	41.0	40.0	41.0	38.0	41.0
16	39.93975625443211	41.0	40.0	41.0	38.0	41.0
17	39.93964631055846	41.0	40.0	41.0	38.0	41.0
18	39.93917355190175	41.0	40.0	41.0	38.0	41.0
19	39.936452441028855	41.0	40.0	41.0	38.0	41.0
20	39.90020394588563	41.0	40.0	41.0	38.0	41.0
21	39.875455579926445	41.0	40.0	41.0	38.0	41.0
22	39.84109811941004	41.0	40.0	41.0	38.0	41.0
23	39.8015623024446	41.0	40.0	41.0	38.0	41.0
24	39.781876851867125	41.0	40.0	41.0	38.0	41.0
25	39.74305567008042	41.0	40.0	41.0	38.0	41.0
26	39.655106068352104	41.0	40.0	41.0	37.0	41.0
27	39.57612238952015	41.0	40.0	41.0	37.0	41.0
28	39.53711430314824	41.0	40.0	41.0	37.0	41.0
29	39.490992848151016	41.0	40.0	41.0	37.0	41.0
30	39.452325587787435	41.0	40.0	41.0	37.0	41.0
31	39.39169154146808	41.0	39.0	41.0	36.0	41.0
32	39.35893376431332	41.0	39.0	41.0	36.0	41.0
33	39.285408798808206	41.0	39.0	41.0	36.0	41.0
34	39.242387761047986	41.0	39.0	41.0	36.0	41.0
35	39.17174332503257	41.0	39.0	41.0	35.0	41.0
36	39.12363738311592	41.0	39.0	41.0	35.0	41.0
37	39.062695493950336	41.0	39.0	41.0	35.0	41.0
38	38.985943675753525	41.0	39.0	41.0	35.0	41.0
39	38.90532183320415	40.0	39.0	41.0	35.0	41.0
40	38.82525520721671	40.0	38.0	41.0	35.0	41.0
41	38.778463094590215	40.0	38.0	41.0	35.0	41.0
42	38.720819521634205	40.0	38.0	41.0	35.0	41.0
43	37.99987906173898	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	4.0
20	13.0
21	23.0
22	43.0
23	64.0
24	104.0
25	147.0
26	219.0
27	307.0
28	417.0
29	567.0
30	752.0
31	979.0
32	1330.0
33	1845.0
34	2729.0
35	4338.0
36	6966.0
37	13401.0
38	35663.0
39	111996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30854098982469	16.475639186195448	12.14330084491867	28.07251897906119
2	21.851344888434454	18.91749262001748	31.352144730115278	27.879017761432788
3	21.370340441204768	19.810236874075784	28.021395077812777	30.798027606906675
4	16.13536289724096	14.994695208096267	33.32783613965071	35.54210575501207
5	17.56573269345999	32.70555381477755	32.92983931702866	16.7988741747338
6	36.25014430133417	33.10629923424092	14.48345619561214	16.160100268812773
7	31.153696038172512	26.932950728653022	19.535377189944533	22.37797604322993
8	28.33363567898588	30.43246422701211	19.243476205397144	21.99042388860487
9	27.999406303082274	12.413213054735557	17.099020949805123	42.488359692377045
10	19.57385754572291	23.024446020306634	29.838217589920347	27.56347884405011
11	40.62591047270369	18.590409595901292	18.51289916497628	22.270780766418742
12	23.74402867336225	23.04588507566887	26.146302312669384	27.063783938299498
13	35.2513042092012	17.33924831373584	20.311031218562924	27.098416258500034
14	24.877550010719528	18.54753148517682	22.33509793250546	34.2398205715982
15	29.46111010329227	23.817691068709422	19.555167087201983	27.166031740796328
16	27.062134780194715	22.886466458872746	21.77988137056033	28.271517390372214
17	27.555233053526173	22.99256229694741	21.576485204303204	27.87571944522321
18	27.005513685263672	21.19333080462424	22.916701024127185	28.884454485984907
19	28.73438109844924	21.737003259835856	22.642391059364193	26.88622458235071
20	29.616130965142297	20.841510408936237	22.306512525355807	27.235846100565663
21	28.051629643067216	21.63750405418034	21.72271055626103	28.588155746491417
22	28.713491762455266	22.422503312059195	20.940459895223487	27.923545030262055
23	28.924034280499804	21.6468492834408	21.65509507396474	27.774021362094654
24	28.456223098108413	21.42641181676754	21.69467486847964	28.4226902166444
25	28.95206996828119	21.675984409958716	21.961838481455217	27.410107140304873
26	28.45292478189884	21.9151123351529	21.855192924012293	27.776769958935965
27	27.7806179945138	21.643550967231228	22.819950415312984	27.755880622941987
28	27.0066131240002	22.28342431188878	21.893123560422403	28.816839003688617
29	27.807004524190397	23.05028283061497	21.951393813458232	27.1913188317364
30	27.589865373726713	21.669937496907828	23.04148732072277	27.698709808642686
31	28.01534816476189	22.16633408644887	22.152041382874042	27.6662763659152
32	26.212818356229146	21.751845682778942	22.540692976235633	29.494642984756283
33	26.703168032719297	21.679832445536555	23.449379091973547	28.1676204297706
34	27.056087867143823	21.26149600628879	23.591206688985274	28.091209437582116
35	26.478882530468194	22.72319980649878	23.710495791898236	27.087421871134786
36	26.72130877187196	21.434107887923215	24.49604476914535	27.34853857105947
37	26.82245713563226	21.092182440863937	24.223933681855414	27.861426741648387
38	25.913771019894345	20.420425372847163	25.714222889215055	27.951580718043438
39	25.339863999428292	19.947116996773147	26.37498556986658	28.338033433931976
40	24.971552022692414	20.591937815745062	27.4991616779634	26.937348483599123
41	23.676962910434224	19.99989005612635	28.136286425779637	28.186860607659792
42	22.43679601563402	20.717273831708912	29.295094854076993	27.550835298580072
43	21.744149611623268	20.61392659047556	28.930630912918954	28.711292884982214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	2.5
4	5.0
5	5.0
6	4.0
7	3.0
8	4.5
9	6.0
10	9.5
11	13.0
12	13.0
13	24.5
14	36.0
15	64.5
16	93.0
17	85.5
18	78.0
19	78.0
20	115.0
21	152.0
22	154.0
23	156.0
24	207.5
25	259.0
26	259.0
27	323.0
28	387.0
29	528.5
30	670.0
31	934.0
32	1198.0
33	1198.0
34	1590.5
35	1983.0
36	2374.5
37	2766.0
38	3542.0
39	4318.0
40	4318.0
41	5167.0
42	6016.0
43	7127.5
44	8239.0
45	9912.0
46	11585.0
47	11585.0
48	13055.5
49	14526.0
50	16169.0
51	17812.0
52	18363.0
53	18914.0
54	18914.0
55	18597.5
56	18281.0
57	17636.0
58	16991.0
59	16233.0
60	15475.0
61	15475.0
62	14172.0
63	12869.0
64	11096.0
65	9323.0
66	8002.0
67	6681.0
68	6681.0
69	5732.5
70	4784.0
71	4012.5
72	3241.0
73	2601.0
74	1961.0
75	1961.0
76	1572.0
77	1183.0
78	960.0
79	737.0
80	632.0
81	527.0
82	527.0
83	434.0
84	341.0
85	242.5
86	144.0
87	111.5
88	79.0
89	79.0
90	62.0
91	45.0
92	30.5
93	16.0
94	12.0
95	8.0
96	8.0
97	6.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	181911.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.98501255192147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.03661829232466	54.82002495618866
2	6.756478390842158	8.51113752507959
3	2.054514420476082	3.8821084968536907
4	0.993733632529972	2.5036130127266727
5	0.6831656218319947	2.1514597633064714
6	0.4277277496994508	1.6164262610175024
7	0.3190321286119479	1.4065969837562835
8	0.21135622119134984	1.0649819395731088
9	0.17646206643788792	1.0003018922575535
>10	1.1963522488616662	14.497774959843523
>50	0.09969601875366506	4.153793327589151
>100	0.044863208439149274	4.391780881807788
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	430	0.23637932835287584	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	331	0.18195711089488817	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	250	0.1374298420656255	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	231	0.12698517406863796	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	211	0.11599078670338792	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	204	0.1121427511255504	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	196	0.1077449961794504	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	193	0.10609583807466287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	5.49719368262502E-4	0.0
20	0.0	0.0	0.0	5.49719368262502E-4	0.0
21	5.49719368262502E-4	0.0	0.0	5.49719368262502E-4	0.0
22	5.49719368262502E-4	0.0	0.0	0.0032983162095750118	0.0
23	5.49719368262502E-4	0.0	0.0	0.004947474314362518	0.0
24	5.49719368262502E-4	0.0	0.0	0.00824579052393753	5.49719368262502E-4
25	5.49719368262502E-4	0.0	0.0	0.009345229260462534	5.49719368262502E-4
26	5.49719368262502E-4	0.0	0.0	0.013193264838300047	5.49719368262502E-4
27	5.49719368262502E-4	0.0	0.0	0.03243344272748762	5.49719368262502E-4
28	5.49719368262502E-4	0.0	0.0	0.11928910291296294	5.49719368262502E-4
29	5.49719368262502E-4	0.0	0.0	0.25781838371511345	5.49719368262502E-4
30	5.49719368262502E-4	0.0	0.0	0.43043026534953904	5.49719368262502E-4
31	5.49719368262502E-4	0.0	0.0	0.9224290999444783	5.49719368262502E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	150	0.0	29.6	1
GTGTTAC	25	0.00548434	29.599998	1
CACTATA	25	0.00548434	29.599998	2
GTAGTTC	25	0.00548434	29.599998	6
TAGTAGT	25	0.00548434	29.599998	4
GCGGTAA	140	0.0	26.42857	23
CTTATAC	160	0.0	25.437502	37
TAATACG	155	0.0	22.67742	27
GGTAATA	165	0.0	22.424242	25
GTAATAC	165	0.0	22.424242	26
CGGTAAT	165	0.0	22.424242	24
AACTCCG	160	0.0	21.968752	5
AATACGG	160	0.0	21.968752	28
ATACGGA	145	0.0	21.689655	29
TAAAGAC	60	3.7041373E-5	21.583334	37
CTAACTC	165	0.0	21.30303	3
CGCGGTA	175	0.0	21.142857	22
TAACTCC	170	0.0	20.676472	4
GGTACTT	45	0.003813624	20.555557	2
AGCCGCG	180	0.0	20.555557	19
>>END_MODULE