FastQCFastQC Report
Fri 10 Feb 2017
ERR1632643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18162
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1280.7047681973350953No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA810.44598612487611494No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT800.44048012333443454RNA PCR Primer, Index 47 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA610.33586609404250634No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA570.3138420878757846No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA560.3083360863341042No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA550.30283008479242374No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT540.29732408325074333No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA530.29181808170906287No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC510.280806078625702No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC470.2587820724589803No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT460.25327607091729987No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG450.2477700693756194No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT440.242264067833939No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC430.23675806629225854No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC420.23125206475057813No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT410.22574606320889767No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC400.22024006166721727No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA400.22024006166721727No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT400.22024006166721727No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA390.2147340601255368No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC370.203722057042176No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA370.203722057042176No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC360.19821605550049554No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC350.1927100539588151No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA350.1927100539588151No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC340.18720405241713467No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA340.18720405241713467No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC340.18720405241713467No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC330.18169805087545426No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC310.1706860477920934No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA310.1706860477920934No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG310.1706860477920934No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG290.15967404470873253No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA280.1541680431670521No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA280.1541680431670521No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC270.14866204162537167No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA270.14866204162537167No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA270.14866204162537167No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT270.14866204162537167No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC270.14866204162537167No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA270.14866204162537167No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT270.14866204162537167No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC270.14866204162537167No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA260.1431560400836912No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA260.1431560400836912No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG260.1431560400836912No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC260.1431560400836912No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG250.13765003854201077No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC250.13765003854201077No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT240.13214403700033034No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG240.13214403700033034No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC240.13214403700033034No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC240.13214403700033034RNA PCR Primer, Index 47 (95% over 23bp)
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT230.12663803545864993No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA230.12663803545864993No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT220.1211320339169695No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT220.1211320339169695No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA210.11562603237528907No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA210.11562603237528907No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC210.11562603237528907No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG210.11562603237528907No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG210.11562603237528907No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG210.11562603237528907No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG200.11012003083360863No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC200.11012003083360863No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT200.11012003083360863No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC200.11012003083360863No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC200.11012003083360863No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC200.11012003083360863No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT200.11012003083360863No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC190.1046140292919282No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA190.1046140292919282No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG190.1046140292919282No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC190.1046140292919282No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG190.1046140292919282No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC190.1046140292919282No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT190.1046140292919282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACC250.005356761629.612
AAACCGC250.005356761629.614
TGCGCTT250.005356761629.626
TGCGTGC250.005356761629.622
GTATCAA250.005356761629.62
GCGCTTT250.005356761629.627
CGTGCGC250.005356761629.624
GGTATCA250.005356761629.61
AACGCAG652.4498986E-622.769235
ACGCAGA652.4498986E-622.769236
CAGAGTA652.4498986E-622.769239
AGAGTAC652.4498986E-622.7692310
CGCAGAG652.4498986E-622.769237
TATCAAC652.4498986E-622.769231
ATCAACG652.4498986E-622.769232
CAACGCA652.4498986E-622.769234
TCAACGC704.661577E-621.1428593
GCAGAGT704.661577E-621.1428598
TAACTCC554.8802412E-420.1818184
CTAACTC554.8802412E-420.1818183
Produced by FastQC (version 0.11.5)