##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632643.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18162
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78845942076864	34.0	31.0	34.0	31.0	34.0
2	32.91592335645854	34.0	33.0	34.0	31.0	34.0
3	32.99267701794957	34.0	33.0	34.0	31.0	34.0
4	36.359817200748815	37.0	37.0	37.0	35.0	37.0
5	36.30591344565576	37.0	37.0	37.0	35.0	37.0
6	36.382116506992624	37.0	37.0	37.0	35.0	37.0
7	36.36587380244467	37.0	37.0	37.0	35.0	37.0
8	36.334214293580004	37.0	37.0	37.0	35.0	37.0
9	38.074826560951436	39.0	38.0	39.0	37.0	39.0
10	38.10494438938443	39.0	38.0	39.0	37.0	39.0
11	38.18527695187755	39.0	38.0	39.0	37.0	39.0
12	38.13594317806409	39.0	38.0	39.0	37.0	39.0
13	38.19381125426715	39.0	38.0	39.0	37.0	39.0
14	39.63170355687699	41.0	40.0	41.0	37.0	41.0
15	39.644477480453695	41.0	40.0	41.0	37.0	41.0
16	39.56970597951767	41.0	39.0	41.0	37.0	41.0
17	39.543882832287196	41.0	39.0	41.0	37.0	41.0
18	39.5599603567889	41.0	39.0	41.0	37.0	41.0
19	39.540358991300515	41.0	39.0	41.0	37.0	41.0
20	39.53226516903425	41.0	39.0	41.0	37.0	41.0
21	39.51315934368461	41.0	39.0	41.0	37.0	41.0
22	39.47450721286202	41.0	39.0	41.0	37.0	41.0
23	39.45105164629446	41.0	39.0	41.0	37.0	41.0
24	39.410637594978525	40.0	39.0	41.0	37.0	41.0
25	39.380684946591785	40.0	39.0	41.0	37.0	41.0
26	39.23962118709393	40.0	39.0	41.0	36.0	41.0
27	39.10109018830525	40.0	39.0	41.0	36.0	41.0
28	39.077194141614356	40.0	39.0	41.0	36.0	41.0
29	39.026814227507984	40.0	39.0	41.0	35.0	41.0
30	38.9859046360533	40.0	39.0	41.0	35.0	41.0
31	38.919777557537714	40.0	39.0	41.0	35.0	41.0
32	38.863341041735495	40.0	38.0	41.0	35.0	41.0
33	38.76924347538817	40.0	38.0	41.0	35.0	41.0
34	38.745072128620194	40.0	38.0	41.0	35.0	41.0
35	38.628124655874906	40.0	38.0	41.0	35.0	41.0
36	38.575211981059354	40.0	38.0	41.0	35.0	41.0
37	38.47874683404911	40.0	38.0	41.0	35.0	41.0
38	38.40942627463936	40.0	38.0	41.0	35.0	41.0
39	38.24165840766435	40.0	38.0	41.0	34.0	41.0
40	38.17327386851668	40.0	37.0	41.0	34.0	41.0
41	38.10180596850567	40.0	37.0	41.0	34.0	41.0
42	37.99955951987666	40.0	37.0	41.0	34.0	41.0
43	37.18935139301839	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	0.0
21	3.0
22	7.0
23	8.0
24	25.0
25	21.0
26	24.0
27	45.0
28	67.0
29	91.0
30	126.0
31	135.0
32	199.0
33	253.0
34	363.0
35	558.0
36	899.0
37	1926.0
38	5057.0
39	8353.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.85871600044048	16.094042506331903	12.575707521198106	28.471533972029512
2	22.97103843189076	19.79407554234115	30.079286422200198	27.155599603567886
3	21.92489813897148	19.02323532650589	29.220350181698052	29.83151635282458
4	15.703116396872591	16.650148662041627	35.519215945380466	32.127518995705316
5	17.789890981169478	32.02290496641339	32.51293910362295	17.674264948794185
6	30.58033256249312	35.38156590683845	16.534522629666338	17.503578901002093
7	28.82942407223874	27.755753771611058	19.337077414381675	24.077744741768527
8	26.566457438608083	28.97808611386411	21.68814007267922	22.767316374848583
9	27.430899680651912	12.10769739015527	17.008038762250855	43.45336416694197
10	21.121021913886135	21.247659949344786	27.41988767756855	30.21143045920053
11	40.430569320559414	18.709393238630106	18.048673053628455	22.811364387182028
12	21.726682083470983	23.609734610725692	26.599493447858162	28.064089857945163
13	38.05748265609515	17.013544763792535	18.340491135337516	26.588481444774803
14	24.204382777227178	20.493337738134567	22.139632199097015	33.16264728554124
15	31.345666776786697	22.32133024997247	19.210439378923024	27.122563594317807
16	24.72194692214514	23.433542561391917	22.662702345556657	29.181808170906287
17	27.458429688360315	23.532650589142165	20.179495650258783	28.82942407223874
18	26.53892743089968	19.463715449840326	22.525052307014644	31.47230481224535
19	28.658738024446645	22.216716220680542	22.640678339389936	26.483867415482877
20	31.362184781411738	19.26549939433983	21.864332122012993	27.50798370223544
21	28.311859927320782	20.53187974892633	21.110009910802773	30.046250412950116
22	28.625702015196563	22.43145028080608	20.493337738134567	28.44950996586279
23	30.393128510075982	20.207025657967183	20.867745842968834	28.532099988987998
24	28.251293910362296	20.218037661050545	22.134126197555336	29.396542231031823
25	29.704878317365928	21.58352604338729	21.6936460742209	27.01794956502588
26	30.156370443783725	22.05153617443013	20.735601805968507	27.056491575817642
27	27.254707631318137	21.56150203722057	24.46316484968616	26.720625481775134
28	26.946371544984032	23.411518555225193	20.12994163638366	29.512168263407112
29	28.317365928862458	23.71434864001762	20.465807730426164	27.502477700693756
30	27.271225635943182	20.575927761259774	24.61733289285321	27.53551370994384
31	29.407554234115185	21.578020041845612	21.9193921374298	27.0950335866094
32	26.390265389274308	20.75211981059355	22.530558308556326	30.327056491575817
33	27.0399735711926	20.54839775355137	24.270454795727343	28.141173879528687
34	27.067503578901004	22.145138200638694	22.238740226847263	28.54861799361304
35	25.867195242814667	23.929082700143155	23.609734610725692	26.593987446316486
36	27.656645743860807	20.66402378592666	24.59530888668649	27.08402158352604
37	28.68626803215505	20.966853870719085	22.761810373306904	27.585067723818963
38	26.69309547406673	20.223543662592224	24.980728994604117	28.10263186873692
39	26.170025327607092	19.061777337297656	25.586389164188965	29.181808170906287
40	26.192049333773816	20.421759718092723	27.392357669860147	25.99383327827332
41	23.659288624600816	20.15196564255038	27.601585728444007	28.5871600044048
42	21.41834599713688	20.438277722717764	30.23345446536725	27.90992181477811
43	20.746613809051865	22.134126197555336	28.06959585948684	29.049664133905956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	2.5
11	4.0
12	4.0
13	8.0
14	12.0
15	17.0
16	22.0
17	22.0
18	22.0
19	22.0
20	25.5
21	29.0
22	20.5
23	12.0
24	15.0
25	18.0
26	18.0
27	16.0
28	14.0
29	14.0
30	14.0
31	17.0
32	20.0
33	20.0
34	35.5
35	51.0
36	45.5
37	40.0
38	94.5
39	149.0
40	149.0
41	222.5
42	296.0
43	418.5
44	541.0
45	924.5
46	1308.0
47	1308.0
48	1514.5
49	1721.0
50	1991.0
51	2261.0
52	2352.0
53	2443.0
54	2443.0
55	2217.5
56	1992.0
57	1998.0
58	2004.0
59	1873.5
60	1743.0
61	1743.0
62	1541.5
63	1340.0
64	1020.5
65	701.0
66	621.5
67	542.0
68	542.0
69	447.0
70	352.0
71	303.5
72	255.0
73	186.0
74	117.0
75	117.0
76	92.0
77	67.0
78	51.5
79	36.0
80	30.0
81	24.0
82	24.0
83	15.5
84	7.0
85	4.0
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	18162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.42341151855522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.27359427776673	45.04459861248761
2	7.798529703953905	8.644422420438277
3	3.4869858931055036	5.797819623389495
4	1.8478044903636002	4.096465147010242
5	1.2418040929862906	3.44125096355027
6	0.794754619511226	2.6428807400066074
7	0.6159348301212001	2.3896046690893074
8	0.4172461752433936	1.850016518004625
9	0.35763957878005165	1.7839444995044598
>10	2.0663620107291876	20.586939764343136
>50	0.08940989469501291	3.0172888448408766
>100	0.009934432743890324	0.7047681973350953
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	128	0.7047681973350953	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	81	0.44598612487611494	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	80	0.44048012333443454	RNA PCR Primer, Index 47 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	61	0.33586609404250634	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	57	0.3138420878757846	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	56	0.3083360863341042	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	55	0.30283008479242374	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	54	0.29732408325074333	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	53	0.29181808170906287	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	51	0.280806078625702	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	47	0.2587820724589803	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	46	0.25327607091729987	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	45	0.2477700693756194	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	44	0.242264067833939	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	43	0.23675806629225854	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	42	0.23125206475057813	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	41	0.22574606320889767	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	40	0.22024006166721727	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	40	0.22024006166721727	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	40	0.22024006166721727	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	39	0.2147340601255368	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	37	0.203722057042176	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	37	0.203722057042176	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	36	0.19821605550049554	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	35	0.1927100539588151	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	35	0.1927100539588151	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	34	0.18720405241713467	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	34	0.18720405241713467	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	34	0.18720405241713467	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	33	0.18169805087545426	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	31	0.1706860477920934	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	31	0.1706860477920934	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	31	0.1706860477920934	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	29	0.15967404470873253	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	28	0.1541680431670521	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	28	0.1541680431670521	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	27	0.14866204162537167	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	27	0.14866204162537167	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	27	0.14866204162537167	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	27	0.14866204162537167	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	27	0.14866204162537167	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	27	0.14866204162537167	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	27	0.14866204162537167	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	27	0.14866204162537167	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	26	0.1431560400836912	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	26	0.1431560400836912	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	26	0.1431560400836912	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	26	0.1431560400836912	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	25	0.13765003854201077	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	25	0.13765003854201077	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	24	0.13214403700033034	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	24	0.13214403700033034	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	24	0.13214403700033034	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	24	0.13214403700033034	RNA PCR Primer, Index 47 (95% over 23bp)
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	23	0.12663803545864993	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	23	0.12663803545864993	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	22	0.1211320339169695	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	22	0.1211320339169695	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	21	0.11562603237528907	No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA	21	0.11562603237528907	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	21	0.11562603237528907	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	21	0.11562603237528907	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	21	0.11562603237528907	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	21	0.11562603237528907	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	20	0.11012003083360863	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	20	0.11012003083360863	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	20	0.11012003083360863	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	20	0.11012003083360863	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC	20	0.11012003083360863	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	20	0.11012003083360863	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	20	0.11012003083360863	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	19	0.1046140292919282	No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA	19	0.1046140292919282	No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG	19	0.1046140292919282	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	19	0.1046140292919282	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	19	0.1046140292919282	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	19	0.1046140292919282	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	19	0.1046140292919282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.005506001541680431	0.0
23	0.0	0.0	0.0	0.005506001541680431	0.0
24	0.0	0.0	0.0	0.005506001541680431	0.0
25	0.0	0.0	0.0	0.011012003083360863	0.0
26	0.0	0.0	0.0	0.016518004625041296	0.0
27	0.0	0.0	0.0	0.05506001541680432	0.0
28	0.0	0.0	0.0	0.18720405241713467	0.0
29	0.0	0.0	0.0	0.2753000770840216	0.0
30	0.0	0.0	0.0	0.49554013875123887	0.0
31	0.0	0.0	0.0	0.9139962559189516	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAAACC	25	0.0053567616	29.6	12
AAACCGC	25	0.0053567616	29.6	14
TGCGCTT	25	0.0053567616	29.6	26
TGCGTGC	25	0.0053567616	29.6	22
GTATCAA	25	0.0053567616	29.6	2
GCGCTTT	25	0.0053567616	29.6	27
CGTGCGC	25	0.0053567616	29.6	24
GGTATCA	25	0.0053567616	29.6	1
AACGCAG	65	2.4498986E-6	22.76923	5
ACGCAGA	65	2.4498986E-6	22.76923	6
CAGAGTA	65	2.4498986E-6	22.76923	9
AGAGTAC	65	2.4498986E-6	22.76923	10
CGCAGAG	65	2.4498986E-6	22.76923	7
TATCAAC	65	2.4498986E-6	22.76923	1
ATCAACG	65	2.4498986E-6	22.76923	2
CAACGCA	65	2.4498986E-6	22.76923	4
TCAACGC	70	4.661577E-6	21.142859	3
GCAGAGT	70	4.661577E-6	21.142859	8
TAACTCC	55	4.8802412E-4	20.181818	4
CTAACTC	55	4.8802412E-4	20.181818	3
>>END_MODULE