FastQCFastQC Report
Fri 10 Feb 2017
ERR1632642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611766
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12880.21053801616958118No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10470.17114386873412385No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7800.12749973028903208No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7120.11638436918691134No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT6700.10951899909442499No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6580.10755746478228603No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6490.10608631404818182No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6300.10298055138729516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAT250.00549435829.65
TAAATTG250.00549435829.65
CAATAGT250.00549435829.61
GGTATCA4750.029.2105261
ATTTATA358.864631E-426.4285722
CGAACTA358.864631E-426.42857224
TACCTTC358.864631E-426.4285727
AGTATTA601.3356985E-624.66666615
TAAGGTA400.001930160123.1250025
ATACGGC1300.022.7692329
AACTCCG6750.022.7481485
GCGGTAA6600.022.70454623
ATACACA1400.022.46428737
GGTAATA6600.022.42424225
GGTCTAG502.7001E-422.21
TAATACG6400.021.96875227
ACGTCAA3400.021.76470629
TTTATTC603.72262E-521.5833323
CGGTAAT6950.021.56115224
GTAACGT3650.021.2876726
Produced by FastQC (version 0.11.5)