##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632642.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	611766
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06018804575606	34.0	33.0	34.0	31.0	34.0
2	33.20516504676625	34.0	33.0	34.0	31.0	34.0
3	33.27504797586005	34.0	34.0	34.0	31.0	34.0
4	36.556093342879464	37.0	37.0	37.0	35.0	37.0
5	36.52589715675602	37.0	37.0	37.0	35.0	37.0
6	36.567890337155056	37.0	37.0	37.0	35.0	37.0
7	36.563985249261975	37.0	37.0	37.0	35.0	37.0
8	36.53986164644652	37.0	37.0	37.0	35.0	37.0
9	38.33318948748378	39.0	39.0	39.0	37.0	39.0
10	38.36399701846785	39.0	39.0	39.0	37.0	39.0
11	38.43696282565556	39.0	39.0	39.0	37.0	39.0
12	38.40263107135735	39.0	39.0	39.0	37.0	39.0
13	38.426205444565404	39.0	39.0	39.0	37.0	39.0
14	40.00381028040133	41.0	40.0	41.0	38.0	41.0
15	40.0012815357506	41.0	40.0	41.0	38.0	41.0
16	39.96773766440109	41.0	40.0	41.0	38.0	41.0
17	39.95785153146792	41.0	40.0	41.0	38.0	41.0
18	39.94842962832194	41.0	40.0	41.0	38.0	41.0
19	39.95968229682591	41.0	40.0	41.0	38.0	41.0
20	39.93187591333941	41.0	40.0	41.0	38.0	41.0
21	39.89724175583475	41.0	40.0	41.0	38.0	41.0
22	39.87598853156272	41.0	40.0	41.0	38.0	41.0
23	39.836376980741	41.0	40.0	41.0	38.0	41.0
24	39.81703298319946	41.0	40.0	41.0	38.0	41.0
25	39.77636220384918	41.0	40.0	41.0	38.0	41.0
26	39.70533504640663	41.0	40.0	41.0	38.0	41.0
27	39.62202214572238	41.0	40.0	41.0	37.0	41.0
28	39.56298977059856	41.0	40.0	41.0	37.0	41.0
29	39.52930041878758	41.0	40.0	41.0	37.0	41.0
30	39.494481550135184	41.0	40.0	41.0	37.0	41.0
31	39.43293514186797	41.0	39.0	41.0	37.0	41.0
32	39.37513036030116	41.0	39.0	41.0	36.0	41.0
33	39.31786500067019	41.0	39.0	41.0	36.0	41.0
34	39.27643739599782	41.0	39.0	41.0	36.0	41.0
35	39.20346995419818	41.0	39.0	41.0	35.0	41.0
36	39.15843476100339	41.0	39.0	41.0	35.0	41.0
37	39.10486198971502	41.0	39.0	41.0	35.0	41.0
38	39.01821938453592	41.0	39.0	41.0	35.0	41.0
39	38.9390338789668	40.0	39.0	41.0	35.0	41.0
40	38.86406567216877	40.0	39.0	41.0	35.0	41.0
41	38.81063838134189	40.0	38.0	41.0	35.0	41.0
42	38.75221735107868	40.0	38.0	41.0	35.0	41.0
43	38.03087291546114	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	6.0
18	12.0
19	22.0
20	33.0
21	78.0
22	129.0
23	223.0
24	374.0
25	524.0
26	761.0
27	956.0
28	1332.0
29	1820.0
30	2526.0
31	3255.0
32	4462.0
33	6012.0
34	9015.0
35	14115.0
36	22711.0
37	43428.0
38	117062.0
39	382909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.275043072024275	16.066600628344823	12.38627187519411	28.272084424436795
2	21.35636828460555	19.160267160973312	31.88604793336014	27.597316621060997
3	21.38938744552656	19.44779539889435	28.369507295273028	30.793309860306067
4	16.257032917814982	14.85617049656241	32.49886394471089	36.38793264091172
5	17.421857376840162	33.14175681551443	32.487585122416085	16.94880068522932
6	36.41424989293292	32.51553698636407	14.511757763589348	16.55845535711367
7	31.348750993026748	27.060673525498313	19.069873121422244	22.5207023600527
8	27.936171673482995	30.983415227390864	19.506804889451196	21.57360820967494
9	28.025748407070676	12.942203391492827	16.777329894109837	42.254718307326655
10	19.302805320988746	23.737670939542244	29.549206722831933	27.41031701663708
11	40.00663652442274	18.99010405939526	18.475037841266104	22.5282215749159
12	23.54772903365012	22.979864850285896	25.932300912440375	27.54010520362361
13	34.76966683339708	17.215732812872897	20.83280208445713	27.181798269272896
14	24.702255437536575	18.73919112863415	21.892030613012164	34.66652282081711
15	29.444428098325176	23.932189758829356	19.44681463173828	27.176567511107187
16	27.180980963309505	23.10687419699689	21.427146981035232	28.28499785865838
17	27.836133423563915	23.023672449923662	21.227233942389738	27.91296018412269
18	26.711520417937578	21.51214680122792	23.10523958507011	28.671093195764392
19	28.878688910465765	21.870453735578636	22.537865785283916	26.71299156867168
20	29.60641814026932	20.791282941516855	22.41919295939951	27.18310595881432
21	27.6566530340032	22.025905329815647	21.47569495526067	28.841746680920483
22	28.562064580248002	22.143924310929343	20.80354253096772	28.490468577854934
23	28.12153666598013	22.086712893491956	21.66089648656512	28.13085395396279
24	28.85024666293975	21.480925713426373	21.51051218930114	28.158315434332735
25	29.151669102238436	21.68443489831079	21.969674679534332	27.19422131991644
26	28.856785110646882	22.020347649264586	21.618723498854138	27.504143741234394
27	27.90249866779128	21.372714403873378	22.91791305826084	27.806873870074504
28	27.078490795500244	22.324385467646128	21.844463405942797	28.75266033091084
29	27.68787412180474	23.125018389384177	21.59976200050346	27.58734548830762
30	27.371576713972335	22.25229908167502	22.84337475439956	27.532749449953087
31	28.193459590758557	21.884347936956285	22.24657793993128	27.675614532353876
32	26.001281535750596	21.829588437409075	22.85236511999686	29.316764906843467
33	26.84049129896071	21.600415845274174	23.207893214072048	28.351199641693064
34	27.073423498527216	21.422733528832918	23.31577760123969	28.188065371400178
35	26.559011125168773	22.440115992062324	23.412219704919853	27.58865317784905
36	26.599059117374946	21.417175848281857	24.518198134580867	27.46556689976233
37	27.232634700195828	20.45324519505824	24.17967000454422	28.134450100201715
38	25.757397436274655	20.4182644998251	25.98673348960223	27.837604574298012
39	25.276331146222574	19.62596809891364	26.721981934268985	28.375718820594802
40	24.86032241085644	20.160322737778824	27.851498775675665	27.12785607568907
41	23.454229231438166	19.779131236453154	28.719150786411795	28.047488745696885
42	22.55960612391012	20.168005413834702	29.27737075940801	27.995017702847168
43	21.558242857563187	20.481033597813543	29.317418751614177	28.64330479300909
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	5.0
2	6.0
3	11.5
4	17.0
5	17.0
6	19.5
7	22.0
8	21.0
9	20.0
10	24.0
11	28.0
12	28.0
13	77.0
14	126.0
15	221.5
16	317.0
17	317.5
18	318.0
19	318.0
20	416.5
21	515.0
22	566.5
23	618.0
24	757.5
25	897.0
26	897.0
27	1098.5
28	1300.0
29	2092.5
30	2885.0
31	3341.5
32	3798.0
33	3798.0
34	5076.0
35	6354.0
36	7116.5
37	7879.0
38	11382.0
39	14885.0
40	14885.0
41	17548.5
42	20212.0
43	22418.5
44	24625.0
45	31119.5
46	37614.0
47	37614.0
48	43322.5
49	49031.0
50	55987.0
51	62943.0
52	67808.0
53	72673.0
54	72673.0
55	66602.5
56	60532.0
57	57801.5
58	55071.0
59	51779.5
60	48488.0
61	48488.0
62	45162.0
63	41836.0
64	36172.0
65	30508.0
66	26510.5
67	22513.0
68	22513.0
69	19284.5
70	16056.0
71	13836.0
72	11616.0
73	9530.5
74	7445.0
75	7445.0
76	5935.5
77	4426.0
78	3603.0
79	2780.0
80	2229.0
81	1678.0
82	1678.0
83	1271.5
84	865.0
85	661.5
86	458.0
87	345.5
88	233.0
89	233.0
90	174.5
91	116.0
92	83.0
93	50.0
94	28.0
95	6.0
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	611766.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.46356846540362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50157364626273	45.38181231355858
2	6.737183782789856	7.069134053048051
3	2.0883896559455533	3.2869312109142075
4	1.0738243857713385	2.2534663673093847
5	0.6465347463550374	1.6959759965329935
6	0.4110001259069652	1.2937519946885747
7	0.32034467791542226	1.17644974596404
8	0.24797021527920501	1.0407521893345157
9	0.19991144959931417	0.9439261220764534
>10	1.4549886033772943	15.378920873916197
>50	0.18602734254267833	6.785504860208875
>100	0.12756160342291448	12.016353374991104
>500	0.0040644628541614825	1.2940158313809373
>1k	6.25301977563305E-4	0.38300506607611723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1288	0.21053801616958118	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1047	0.17114386873412385	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	780	0.12749973028903208	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	712	0.11638436918691134	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	670	0.10951899909442499	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	658	0.10755746478228603	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	649	0.10608631404818182	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	630	0.10298055138729516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.6346119267824627E-4	0.0	0.0	0.0	0.0
7	1.6346119267824627E-4	0.0	0.0	0.0	0.0
8	1.6346119267824627E-4	0.0	0.0	0.0	0.0
9	1.6346119267824627E-4	0.0	0.0	0.0	0.0
10	1.6346119267824627E-4	0.0	0.0	1.6346119267824627E-4	0.0
11	1.6346119267824627E-4	0.0	0.0	1.6346119267824627E-4	0.0
12	1.6346119267824627E-4	0.0	0.0	1.6346119267824627E-4	0.0
13	1.6346119267824627E-4	0.0	0.0	1.6346119267824627E-4	0.0
14	1.6346119267824627E-4	0.0	0.0	3.2692238535649254E-4	0.0
15	1.6346119267824627E-4	0.0	0.0	4.903835780347387E-4	0.0
16	1.6346119267824627E-4	0.0	0.0	9.807671560694774E-4	0.0
17	1.6346119267824627E-4	0.0	0.0	0.0014711507341042163	0.0
18	1.6346119267824627E-4	0.0	0.0	0.0017980731194607088	0.0
19	1.6346119267824627E-4	0.0	0.0	0.002451917890173694	0.0
20	1.6346119267824627E-4	0.0	0.0	0.003759607431599664	0.0
21	1.6346119267824627E-4	0.0	0.0	0.004903835780347388	0.0
22	1.6346119267824627E-4	0.0	0.0	0.006701908899808096	0.0
23	1.6346119267824627E-4	0.0	0.0	0.007519214863199328	0.0
24	1.6346119267824627E-4	0.0	0.0	0.009644210368016529	0.0
25	3.2692238535649254E-4	0.0	0.0	0.010461516331407761	0.0
26	3.2692238535649254E-4	0.0	0.0	0.013894201377650931	0.0
27	3.2692238535649254E-4	0.0	0.0	0.03694222954528365	0.0
28	3.2692238535649254E-4	0.0	0.0	0.13518240634490966	0.0
29	3.2692238535649254E-4	0.0	0.0	0.2889993886551394	0.0
30	3.2692238535649254E-4	0.0	0.0	0.47861437216190506	0.0
31	3.2692238535649254E-4	0.0	0.0	1.0160747736879787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTAT	25	0.005494358	29.6	5
TAAATTG	25	0.005494358	29.6	5
CAATAGT	25	0.005494358	29.6	1
GGTATCA	475	0.0	29.210526	1
ATTTATA	35	8.864631E-4	26.428572	2
CGAACTA	35	8.864631E-4	26.428572	24
TACCTTC	35	8.864631E-4	26.428572	7
AGTATTA	60	1.3356985E-6	24.666666	15
TAAGGTA	40	0.0019301601	23.125002	5
ATACGGC	130	0.0	22.76923	29
AACTCCG	675	0.0	22.748148	5
GCGGTAA	660	0.0	22.704546	23
ATACACA	140	0.0	22.464287	37
GGTAATA	660	0.0	22.424242	25
GGTCTAG	50	2.7001E-4	22.2	1
TAATACG	640	0.0	21.968752	27
ACGTCAA	340	0.0	21.764706	29
TTTATTC	60	3.72262E-5	21.583332	3
CGGTAAT	695	0.0	21.561152	24
GTAACGT	365	0.0	21.28767	26
>>END_MODULE