FastQCFastQC Report
Fri 10 Feb 2017
ERR1632641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15327
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA380.24792849220330138No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA340.22183075618190123No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT270.17615971814445097Illumina PCR Primer Index 11 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA210.13701311411235076No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA200.1304886801070007No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA200.1304886801070007No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC190.12396424610165069No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA190.12396424610165069No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA190.12396424610165069No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT180.11743981209630064No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT170.11091537809095062No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC170.11091537809095062No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA170.11091537809095062No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT160.10439094408560058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200.001785764937.01
GTATCAA250.005330612429.62
Produced by FastQC (version 0.11.5)