FastQCFastQC Report
Fri 10 Feb 2017
ERR1632639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621597
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT13980.22490456035019474No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT12960.20849521474524493No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11250.18098542946635843No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10000.16087593730342972No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8990.14462746763578332No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8960.14414483982387302No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8720.14028381732859072No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7320.11776118610611055No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7210.11599155079577282No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7070.11373928767352481No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6970.1121305283004905No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6870.1105217689274562No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC6600.1061781186202636No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6540.10521286299644303No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG6410.10312147581149846No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT6340.10199534425037445No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6310.10151271643846416No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT6270.10086921268925043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACG251.2329732E-437.04
ATACGGC603.45608E-1133.91666429
GGTATCA7700.030.5129891
GATAGAG250.005494424629.67
TAAGGTA405.9358063E-527.7500025
CGTTATT358.864795E-426.4285710
TGTTACG358.864795E-426.4285716
CCGTTTA358.864795E-426.4285727
TAAACCA659.377254E-825.6153855
GACCGTT400.001930194323.1250027
TGCGAGT400.001930194323.1250028
CGTCGTA400.001930194323.12500213
TTACGAG400.001930194323.1250026
AGTCGGT1155.456968E-1222.5217411
GCGGTAA4700.021.64893723
GTGATTC1301.8189894E-1221.34615316
ATCTTAC806.94954E-720.81251
CAGTCGG1251.8189894E-1120.7210
TAGGACC1251.8189894E-1120.724
GACAGGC4850.020.5979397
Produced by FastQC (version 0.11.5)