##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632639.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621597
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01306151734966	34.0	33.0	34.0	31.0	34.0
2	33.16296732448838	34.0	33.0	34.0	31.0	34.0
3	33.2286867536362	34.0	33.0	34.0	31.0	34.0
4	36.52809135179224	37.0	37.0	37.0	35.0	37.0
5	36.503306804891274	37.0	37.0	37.0	35.0	37.0
6	36.54300133366152	37.0	37.0	37.0	35.0	37.0
7	36.53869307606053	37.0	37.0	37.0	35.0	37.0
8	36.506680373296525	37.0	37.0	37.0	35.0	37.0
9	38.311626343112984	39.0	39.0	39.0	37.0	39.0
10	38.33967184526309	39.0	39.0	39.0	37.0	39.0
11	38.41177322284374	39.0	39.0	39.0	37.0	39.0
12	38.3765108261462	39.0	39.0	39.0	37.0	39.0
13	38.40269981997982	39.0	39.0	39.0	37.0	39.0
14	39.95841678772581	41.0	40.0	41.0	38.0	41.0
15	39.94982279515506	41.0	40.0	41.0	38.0	41.0
16	39.92297903625661	41.0	40.0	41.0	38.0	41.0
17	39.91245453243822	41.0	40.0	41.0	38.0	41.0
18	39.889899726028275	41.0	40.0	41.0	38.0	41.0
19	39.896812565054205	41.0	40.0	41.0	38.0	41.0
20	39.859280208881316	41.0	40.0	41.0	38.0	41.0
21	39.83676883897445	41.0	40.0	41.0	38.0	41.0
22	39.80459043399502	41.0	40.0	41.0	38.0	41.0
23	39.76612177986702	41.0	40.0	41.0	38.0	41.0
24	39.75159146520978	41.0	40.0	41.0	38.0	41.0
25	39.695965392368365	41.0	40.0	41.0	37.0	41.0
26	39.61408436655904	41.0	40.0	41.0	37.0	41.0
27	39.54238035254353	41.0	40.0	41.0	37.0	41.0
28	39.49003454006374	41.0	40.0	41.0	37.0	41.0
29	39.45217560573812	41.0	39.0	41.0	37.0	41.0
30	39.40027541960466	41.0	39.0	41.0	36.0	41.0
31	39.357673862647346	41.0	39.0	41.0	36.0	41.0
32	39.304406231046805	41.0	39.0	41.0	36.0	41.0
33	39.23745288345986	41.0	39.0	41.0	35.0	41.0
34	39.19346457592299	41.0	39.0	41.0	35.0	41.0
35	39.09594480024839	41.0	39.0	41.0	35.0	41.0
36	39.06601061459434	41.0	39.0	41.0	35.0	41.0
37	39.010220448296884	41.0	39.0	41.0	35.0	41.0
38	38.94446401768348	40.0	39.0	41.0	35.0	41.0
39	38.8704498252083	40.0	39.0	41.0	35.0	41.0
40	38.76224306101864	40.0	38.0	41.0	35.0	41.0
41	38.714564259480014	40.0	38.0	41.0	35.0	41.0
42	38.64619198612606	40.0	38.0	41.0	35.0	41.0
43	37.89544029330901	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	6.0
18	5.0
19	22.0
20	35.0
21	59.0
22	122.0
23	230.0
24	357.0
25	504.0
26	748.0
27	1033.0
28	1438.0
29	1983.0
30	2706.0
31	3518.0
32	4795.0
33	6450.0
34	9831.0
35	17020.0
36	25365.0
37	47112.0
38	120736.0
39	377518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84770196767359	15.230768488264903	11.938442431350216	28.983087112711292
2	21.253159201218796	17.787087132016403	32.69948214035782	28.26027152640698
3	21.219857882196987	19.13973201286364	28.103900115348047	31.536509989591327
4	15.729001266093626	14.598365178725123	32.55453292084743	37.11810063433382
5	17.374762104707713	32.75659309810054	32.774772079015825	17.093872718175923
6	36.91314469020925	32.40009202103614	14.531119036932289	16.15564425182232
7	32.128855190742556	26.63140266120976	19.072968498882716	22.166773649164973
8	28.823498182906288	30.75513556210857	18.873964964438375	21.54740129054677
9	28.950107545564087	12.367981183950373	16.415619766504662	42.266291503980874
10	20.069273178602856	22.645218686705373	28.645086768436784	28.640421366254987
11	40.3394804028977	18.797548894219247	18.102886596943037	22.760084105940024
12	24.591495776202265	22.554806409940845	25.454916931709775	27.39878088214712
13	34.72764508194216	16.766490185763445	20.364641399491955	28.141223332802447
14	24.484995905707397	18.597097476339172	22.430931938217206	34.48697467973623
15	30.301626294850198	22.751396805325637	19.55945733328829	27.38751956653587
16	26.863224886863996	22.900367923268615	20.631534579478345	29.604872610389044
17	27.70508866677284	22.975014358177404	21.125906334811784	28.19399064023797
18	27.20009909957738	20.444918492206366	22.84245258583938	29.512529822376877
19	29.413269369060664	21.028898144617816	21.68157182225783	27.876260664063697
20	29.37240688098559	20.518599671491337	22.203453362870153	27.90554008465292
21	29.481802518351923	20.961812878762284	20.825872711740885	28.730511891144907
22	30.09715297853754	22.01651552372357	19.967599586227088	27.918731911511802
23	29.29406029951882	21.841160752062834	20.896658124154396	27.968120824263952
24	29.436757255906965	20.750100145270974	20.936877108480253	28.87626549034181
25	28.667448523721962	21.024554494310625	22.160660363547443	28.14733661841997
26	29.29808219795141	21.394247398233905	21.84293038737317	27.464740016441517
27	29.14492830563854	20.69234568377904	22.423370769163945	27.739355241418473
28	27.236457061407954	21.438488280992345	22.16757802885149	29.157476628748206
29	28.798562412624257	21.792737094934502	21.8443782708089	27.56432222163234
30	27.70814530958161	20.67078830818038	23.043064879656754	28.57800150258125
31	27.829767518183	21.326840380503768	22.178195840713517	28.665196260599714
32	26.142983315556545	21.368668124202657	22.590038240210298	29.898310320030504
33	27.39427635590262	21.154542251651794	23.687533884494293	27.763647507951294
34	27.573170398184033	21.184786927864838	23.332319814928322	27.909722859022807
35	27.435782347726906	22.013458880914804	23.75671053753477	26.79404823382352
36	27.156340844630844	20.90438016914496	23.443163335730386	28.49611565049381
37	26.196555002678583	20.825068332054368	24.318006682786436	28.66036998248061
38	26.54259914381826	19.836646573262097	25.346808301841868	28.273945981077773
39	25.211350762632378	19.105143686343403	26.76669932448194	28.91680622654228
40	25.13010841429415	19.743821157438017	28.201873561165836	26.924196867101998
41	23.53051896968615	18.83680262292128	28.845055558504946	28.787622848887622
42	21.608051518910163	19.860134460108398	29.99580113803638	28.536012882945062
43	21.11770166200931	19.576188430767843	30.15345955659374	29.152650350629106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	11.0
4	18.0
5	18.0
6	32.0
7	46.0
8	36.0
9	26.0
10	46.5
11	67.0
12	67.0
13	123.5
14	180.0
15	374.5
16	569.0
17	547.0
18	525.0
19	525.0
20	576.5
21	628.0
22	529.5
23	431.0
24	513.5
25	596.0
26	596.0
27	766.5
28	937.0
29	1338.0
30	1739.0
31	2362.0
32	2985.0
33	2985.0
34	4455.5
35	5926.0
36	6941.0
37	7956.0
38	10732.5
39	13509.0
40	13509.0
41	15847.0
42	18185.0
43	21789.5
44	25394.0
45	31436.5
46	37479.0
47	37479.0
48	42555.0
49	47631.0
50	53875.0
51	60119.0
52	65276.5
53	70434.0
54	70434.0
55	66540.0
56	62646.0
57	59435.0
58	56224.0
59	53074.5
60	49925.0
61	49925.0
62	47947.5
63	45970.0
64	38166.5
65	30363.0
66	26185.0
67	22007.0
68	22007.0
69	18622.5
70	15238.0
71	13872.0
72	12506.0
73	12719.0
74	12932.0
75	12932.0
76	12471.0
77	12010.0
78	7930.0
79	3850.0
80	2504.5
81	1159.0
82	1159.0
83	932.5
84	706.0
85	527.5
86	349.0
87	259.5
88	170.0
89	170.0
90	130.5
91	91.0
92	66.5
93	42.0
94	28.5
95	15.0
96	15.0
97	9.0
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	621597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.05418516591117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.79882604451687	36.5094435837252
2	6.781980306363172	5.83985271803446
3	2.3375291055756184	3.019212328264782
4	1.2520495989257527	2.1562390107621674
5	0.8025834539999354	1.7277288319804887
6	0.5578016095655761	1.4409416270447761
7	0.41283153053884175	1.2441887610702065
8	0.31924991827653443	1.0996036076543938
9	0.27699018773536144	1.0733028148708876
>10	1.99234221805877	17.38878687924578
>50	0.24685079828015696	7.611105319313275
>100	0.21046093887894293	17.582328432677635
>500	0.009378829718312966	2.6904244571094966
>1k	0.0011254595661975557	0.616841628246453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	1398	0.22490456035019474	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	1296	0.20849521474524493	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1125	0.18098542946635843	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1000	0.16087593730342972	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	899	0.14462746763578332	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	896	0.14414483982387302	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	872	0.14028381732859072	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	732	0.11776118610611055	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	721	0.11599155079577282	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	707	0.11373928767352481	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	697	0.1121305283004905	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	687	0.1105217689274562	No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC	660	0.1061781186202636	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	654	0.10521286299644303	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	641	0.10312147581149846	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	634	0.10199534425037445	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	631	0.10151271643846416	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	627	0.10086921268925043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.6087593730342972E-4	0.0	0.0	0.0	0.0
6	1.6087593730342972E-4	0.0	0.0	0.0	0.0
7	1.6087593730342972E-4	0.0	0.0	0.0	0.0
8	1.6087593730342972E-4	0.0	0.0	0.0	0.0
9	1.6087593730342972E-4	0.0	0.0	0.0	0.0
10	1.6087593730342972E-4	0.0	0.0	0.0	0.0
11	1.6087593730342972E-4	0.0	0.0	0.0	0.0
12	1.6087593730342972E-4	0.0	0.0	0.0	0.0
13	1.6087593730342972E-4	0.0	0.0	0.0	0.0
14	1.6087593730342972E-4	0.0	0.0	1.6087593730342972E-4	0.0
15	1.6087593730342972E-4	0.0	0.0	6.435037492137189E-4	0.0
16	1.6087593730342972E-4	0.0	0.0	0.0016087593730342972	0.0
17	1.6087593730342972E-4	0.0	0.0	0.002895766871461735	0.0
18	1.6087593730342972E-4	0.0	0.0	0.0037001465579788832	0.0
19	1.6087593730342972E-4	0.0	0.0	0.004665402181799462	0.0
20	1.6087593730342972E-4	0.0	0.0	0.007239417178654337	0.0
21	1.6087593730342972E-4	0.0	0.0	0.012548323109667518	0.0
22	1.6087593730342972E-4	0.0	0.0	0.019305112476411566	0.0
23	1.6087593730342972E-4	0.0	0.0	0.0238096387209076	0.0
24	3.2175187460685943E-4	0.0	0.0	0.027509785278886482	0.0
25	3.2175187460685943E-4	0.0	0.0	0.030566428087651646	0.0
26	3.2175187460685943E-4	0.0	0.0	0.036518837767878544	0.0
27	4.826278119102891E-4	0.0	0.0	0.06113285617530329	0.0
28	4.826278119102891E-4	0.0	0.0	0.20994309818097578	0.0
29	4.826278119102891E-4	0.0	0.0	0.41666867761588294	0.0
30	4.826278119102891E-4	0.0	0.0	0.6922491582166581	0.0
31	4.826278119102891E-4	0.0	0.0	1.4334046013735589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTACG	25	1.2329732E-4	37.0	4
ATACGGC	60	3.45608E-11	33.916664	29
GGTATCA	770	0.0	30.512989	1
GATAGAG	25	0.0054944246	29.6	7
TAAGGTA	40	5.9358063E-5	27.750002	5
CGTTATT	35	8.864795E-4	26.42857	10
TGTTACG	35	8.864795E-4	26.42857	16
CCGTTTA	35	8.864795E-4	26.42857	27
TAAACCA	65	9.377254E-8	25.615385	5
GACCGTT	40	0.0019301943	23.125002	7
TGCGAGT	40	0.0019301943	23.125002	8
CGTCGTA	40	0.0019301943	23.125002	13
TTACGAG	40	0.0019301943	23.125002	6
AGTCGGT	115	5.456968E-12	22.52174	11
GCGGTAA	470	0.0	21.648937	23
GTGATTC	130	1.8189894E-12	21.346153	16
ATCTTAC	80	6.94954E-7	20.8125	1
CAGTCGG	125	1.8189894E-11	20.72	10
TAGGACC	125	1.8189894E-11	20.72	4
GACAGGC	485	0.0	20.597939	7
>>END_MODULE