FastQCFastQC Report
Fri 10 Feb 2017
ERR1632638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53889
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT2260.41938057859674516Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2160.4008239158269777No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1270.23566961717604706No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1220.2263912857911633No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1090.20226762419046557No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1050.19484495908255858No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1020.18927796025162835No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1010.1874222939746516No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA980.18185529514372134No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT980.18185529514372134No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC970.1799996288667446No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA830.15402030098907013No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA820.15216463471209338No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT800.1484533021581399No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC800.1484533021581399No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT780.1447419696041864No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT770.14288630332720964No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA750.13917497077325613No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC740.13731930449627938No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA670.12432964055744215No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC670.12432964055744215No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA660.1224739742804654No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT650.12061830800348865No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC640.1187626417265119No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG620.1150513091725584No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC610.11319564289558165No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC610.11319564289558165No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT610.11319564289558165No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC600.1113399766186049No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA590.10948431034162817No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC560.10391731151069791No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC540.10020597895674442Illumina PCR Primer Index 10 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA900.032.888891
TTCTTCT352.3466095E-531.71428715
TCTTCTG352.3466095E-531.71428716
AACGTCA405.8372465E-527.75000228
CAATGAG405.8372465E-527.75000233
TAACGTC405.8372465E-527.75000227
TTCTGCG405.8372465E-527.75000218
GTAACGT405.8372465E-527.75000226
CTTCTGC405.8372465E-527.75000217
GGTAACG405.8372465E-527.75000225
ACGTCAA451.3004779E-424.66666629
GGGTAAC451.3004779E-424.66666624
TCAATGA451.3004779E-424.66666632
TCTGCGG451.3004779E-424.66666619
CTGCGGG451.3004779E-424.66666620
GCTTCTT451.3004779E-424.66666613
TGCGGGT451.3004779E-424.66666621
TACGGGT400.001907514423.12500216
GTATCAA1300.022.769232
CGTCAAT502.65605E-422.230
Produced by FastQC (version 0.11.5)