##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632638.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53889
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.868822950880514	34.0	31.0	34.0	31.0	34.0
2	33.03295663307911	34.0	33.0	34.0	31.0	34.0
3	33.10603277106645	34.0	33.0	34.0	31.0	34.0
4	36.44272486036111	37.0	37.0	37.0	35.0	37.0
5	36.40921152739891	37.0	37.0	37.0	35.0	37.0
6	36.452077418397074	37.0	37.0	37.0	35.0	37.0
7	36.46029802000408	37.0	37.0	37.0	35.0	37.0
8	36.422516654604834	37.0	37.0	37.0	35.0	37.0
9	38.22384902299171	39.0	39.0	39.0	37.0	39.0
10	38.224795412792965	39.0	39.0	39.0	37.0	39.0
11	38.308597301861234	39.0	39.0	39.0	37.0	39.0
12	38.24242424242424	39.0	39.0	39.0	37.0	39.0
13	38.28621796656089	39.0	39.0	39.0	37.0	39.0
14	39.78620868822951	41.0	40.0	41.0	38.0	41.0
15	39.78301694223311	41.0	40.0	41.0	38.0	41.0
16	39.73237580953441	41.0	40.0	41.0	38.0	41.0
17	39.73662528530869	41.0	40.0	41.0	38.0	41.0
18	39.735604668856354	41.0	40.0	41.0	38.0	41.0
19	39.739186104770916	41.0	40.0	41.0	38.0	41.0
20	39.742470634081165	41.0	40.0	41.0	38.0	41.0
21	39.69893670322329	41.0	40.0	41.0	38.0	41.0
22	39.6589099816289	41.0	40.0	41.0	37.0	41.0
23	39.60314721000575	41.0	40.0	41.0	37.0	41.0
24	39.58423797064336	41.0	40.0	41.0	37.0	41.0
25	39.54525042216408	41.0	39.0	41.0	37.0	41.0
26	39.446065059659674	41.0	39.0	41.0	37.0	41.0
27	39.34305702462469	41.0	39.0	41.0	36.0	41.0
28	39.306611738944866	41.0	39.0	41.0	36.0	41.0
29	39.26389430124886	41.0	39.0	41.0	36.0	41.0
30	39.24574588506003	40.0	39.0	41.0	36.0	41.0
31	39.1601996696914	40.0	39.0	41.0	36.0	41.0
32	39.106311121008	40.0	39.0	41.0	35.0	41.0
33	39.03117519345321	40.0	39.0	41.0	35.0	41.0
34	39.00250514947392	40.0	39.0	41.0	35.0	41.0
35	38.90855276587059	40.0	39.0	41.0	35.0	41.0
36	38.859748742786095	40.0	38.0	41.0	35.0	41.0
37	38.83460446473306	40.0	38.0	41.0	35.0	41.0
38	38.76735511885543	40.0	38.0	41.0	35.0	41.0
39	38.64380485813431	40.0	38.0	41.0	35.0	41.0
40	38.55927925921802	40.0	38.0	41.0	35.0	41.0
41	38.496223719126355	40.0	38.0	41.0	35.0	41.0
42	38.439124867783775	40.0	38.0	41.0	35.0	41.0
43	37.69346248770621	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	4.0
21	14.0
22	7.0
23	16.0
24	40.0
25	53.0
26	85.0
27	115.0
28	135.0
29	196.0
30	260.0
31	374.0
32	472.0
33	640.0
34	976.0
35	1472.0
36	2405.0
37	4718.0
38	13000.0
39	28903.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.30753957208336	15.9457403180612	12.659355341535377	28.087364768320068
2	22.546345265267494	18.78862105438958	30.071072018408206	28.59396166193472
3	21.51088348271447	18.940785689101673	28.503034014362854	31.045296813821004
4	15.908626992521665	15.257288129302825	33.46508563899868	35.36899923917682
5	17.50264432444469	32.74694278980868	32.37580953441333	17.374603351333295
6	34.778897363098224	33.416838315797285	14.71172224387166	17.09254207723283
7	30.00241236616007	27.04262465438216	19.066970995936092	23.887991983521683
8	28.369426042420532	28.91870326040565	20.159958433075396	22.551912264098426
9	27.640149195568668	12.037707138748168	16.775223143869805	43.54692052181336
10	20.479133032715396	21.820779750969585	28.222828406539367	29.477258809775652
11	40.70403978548498	18.54367310582865	17.380170350164224	23.372116758522147
12	23.22180779008703	23.19397279593238	25.26675202731541	28.31746738666518
13	36.75703761435544	16.8475941286719	19.447382582716326	26.94798567425634
14	24.838093117333777	19.298929280558184	21.753975764998422	34.109001837109616
15	30.41808161220286	23.162426469223774	18.745940730019115	27.673551188554253
16	26.51004843288983	23.351704429475404	21.447790829297258	28.69045630833751
17	28.115199762474717	23.060364823990053	20.286143739909814	28.538291673625416
18	27.03520198927425	20.02263912857912	22.100985358793075	30.84117352335356
19	29.65354710608844	21.603666796563306	21.594388465178422	27.14839763216983
20	32.30158288333426	19.213568631817253	20.829853959064	27.65499452578448
21	28.992929911484715	20.547792684963536	20.605318339549818	29.853959064001927
22	29.42158882146635	22.331087977138193	19.846350832266324	28.400972369129136
23	29.840969400063095	20.594184341887956	20.668410992967022	28.896435265081927
24	28.545714338733326	20.50511236059307	21.63150179071796	29.31767150995565
25	29.55705245968565	21.05438957857819	21.275213865538422	28.11334409619774
26	29.06158956373286	21.729852103397725	21.323461188739817	27.8850971441296
27	27.53808755033495	21.414388836311677	23.407374417784705	27.640149195568668
28	27.358087921468204	22.13253168550168	21.046966913470282	29.462413479559835
29	27.56406687821262	23.030674163558427	21.03212158325447	28.373137374974483
30	27.781179832618903	20.662843994136097	23.127168809961216	28.428807363283788
31	28.47519902020821	21.68717177902726	21.800367421922843	28.037261778841692
32	26.608398745569595	20.514390691977955	22.331087977138193	30.546122585314254
33	26.641800738555176	21.47562582345191	23.396240420122847	28.486333017870063
34	27.385922915622857	21.045111247193304	22.94345784854052	28.625507988643324
35	26.022008202044944	22.197480005195867	24.11252760303587	27.66798418972332
36	26.884893020839133	20.789029300970512	24.56716584089517	27.75891183729518
37	26.730872719850062	19.731299523093767	24.802835458071222	28.734992298984952
38	25.806750913915643	19.328619940989814	25.838297240624247	29.026331904470297
39	25.292731355193084	18.469446454749576	27.346953923806343	28.890868266250997
40	24.4372692015068	19.07253799476702	29.237877860045646	27.252314943680528
41	22.69294290114866	18.790476720666554	29.267568520477276	29.249011857707508
42	20.86696728460354	19.350887936313534	31.50364638423426	28.278498394848672
43	20.256453079478188	20.319545732895396	30.290040639091465	29.133960548534954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	2.5
9	2.0
10	4.0
11	6.0
12	6.0
13	10.0
14	14.0
15	25.0
16	36.0
17	33.5
18	31.0
19	31.0
20	39.0
21	47.0
22	42.0
23	37.0
24	43.0
25	49.0
26	49.0
27	58.5
28	68.0
29	92.5
30	117.0
31	151.0
32	185.0
33	185.0
34	252.5
35	320.0
36	376.0
37	432.0
38	644.5
39	857.0
40	857.0
41	1028.0
42	1199.0
43	1510.5
44	1822.0
45	2634.0
46	3446.0
47	3446.0
48	3978.5
49	4511.0
50	5064.5
51	5618.0
52	5813.0
53	6008.0
54	6008.0
55	5833.5
56	5659.0
57	5420.0
58	5181.0
59	5206.0
60	5231.0
61	5231.0
62	4799.5
63	4368.0
64	3653.0
65	2938.0
66	2482.0
67	2026.0
68	2026.0
69	1762.5
70	1499.0
71	1262.5
72	1026.0
73	780.5
74	535.0
75	535.0
76	401.5
77	268.0
78	209.0
79	150.0
80	128.0
81	106.0
82	106.0
83	76.0
84	46.0
85	36.5
86	27.0
87	19.5
88	12.0
89	12.0
90	7.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	53889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.845849802371546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.18123837363805	54.16133162612036
2	6.357220893495143	7.990498988661879
3	2.4625741872619367	4.642877024995825
4	1.1810907372958928	2.9690660431627975
5	0.9094398677178375	2.857726066544193
6	0.5226326512534326	1.970717586149307
7	0.3956653969941241	1.74061496780419
8	0.3129890453834116	1.5736050028762827
9	0.24212360114565804	1.3694817124088403
>10	1.334632533144359	15.275844792072593
>50	0.07677089792423303	3.392157954313496
>100	0.023621814745917857	2.0560782348902373
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	226	0.41938057859674516	Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	216	0.4008239158269777	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	127	0.23566961717604706	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	122	0.2263912857911633	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	109	0.20226762419046557	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	105	0.19484495908255858	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	102	0.18927796025162835	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	101	0.1874222939746516	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	98	0.18185529514372134	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	98	0.18185529514372134	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	97	0.1799996288667446	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	83	0.15402030098907013	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	82	0.15216463471209338	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	80	0.1484533021581399	No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	80	0.1484533021581399	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	78	0.1447419696041864	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	77	0.14288630332720964	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	75	0.13917497077325613	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	74	0.13731930449627938	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	67	0.12432964055744215	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	67	0.12432964055744215	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	66	0.1224739742804654	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	65	0.12061830800348865	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	64	0.1187626417265119	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	62	0.1150513091725584	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	61	0.11319564289558165	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	61	0.11319564289558165	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	61	0.11319564289558165	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	60	0.1113399766186049	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	59	0.10948431034162817	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	56	0.10391731151069791	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	54	0.10020597895674442	Illumina PCR Primer Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0018556662769767486	0.0	0.0	0.0	0.0018556662769767486
11	0.0018556662769767486	0.0	0.0	0.0	0.0018556662769767486
12	0.0018556662769767486	0.0	0.0	0.0	0.0018556662769767486
13	0.003711332553953497	0.0	0.0	0.0	0.0018556662769767486
14	0.003711332553953497	0.0	0.0	0.0	0.0018556662769767486
15	0.003711332553953497	0.0	0.0	0.0	0.0018556662769767486
16	0.003711332553953497	0.0	0.0	0.0018556662769767486	0.0018556662769767486
17	0.003711332553953497	0.0	0.0	0.0018556662769767486	0.0018556662769767486
18	0.003711332553953497	0.0	0.0	0.0018556662769767486	0.0018556662769767486
19	0.003711332553953497	0.0	0.0	0.0018556662769767486	0.0018556662769767486
20	0.003711332553953497	0.0	0.0	0.0018556662769767486	0.0018556662769767486
21	0.003711332553953497	0.0	0.0	0.0018556662769767486	0.0018556662769767486
22	0.003711332553953497	0.0	0.0	0.003711332553953497	0.0018556662769767486
23	0.003711332553953497	0.0	0.0	0.003711332553953497	0.0018556662769767486
24	0.003711332553953497	0.0	0.0	0.003711332553953497	0.0018556662769767486
25	0.003711332553953497	0.0	0.0	0.005566998830930246	0.0018556662769767486
26	0.003711332553953497	0.0	0.0	0.016700996492790738	0.0018556662769767486
27	0.003711332553953497	0.0	0.0	0.05010298947837221	0.0018556662769767486
28	0.003711332553953497	0.0	0.0	0.22267995323720982	0.0018556662769767486
29	0.003711332553953497	0.0	0.0	0.49546289595279186	0.0018556662769767486
30	0.003711332553953497	0.0	0.0	0.8888641466718625	0.0018556662769767486
31	0.003711332553953497	0.0	0.0	1.653398652786283	0.0018556662769767486
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	90	0.0	32.88889	1
TTCTTCT	35	2.3466095E-5	31.714287	15
TCTTCTG	35	2.3466095E-5	31.714287	16
AACGTCA	40	5.8372465E-5	27.750002	28
CAATGAG	40	5.8372465E-5	27.750002	33
TAACGTC	40	5.8372465E-5	27.750002	27
TTCTGCG	40	5.8372465E-5	27.750002	18
GTAACGT	40	5.8372465E-5	27.750002	26
CTTCTGC	40	5.8372465E-5	27.750002	17
GGTAACG	40	5.8372465E-5	27.750002	25
ACGTCAA	45	1.3004779E-4	24.666666	29
GGGTAAC	45	1.3004779E-4	24.666666	24
TCAATGA	45	1.3004779E-4	24.666666	32
TCTGCGG	45	1.3004779E-4	24.666666	19
CTGCGGG	45	1.3004779E-4	24.666666	20
GCTTCTT	45	1.3004779E-4	24.666666	13
TGCGGGT	45	1.3004779E-4	24.666666	21
TACGGGT	40	0.0019075144	23.125002	16
GTATCAA	130	0.0	22.76923	2
CGTCAAT	50	2.65605E-4	22.2	30
>>END_MODULE