ERR1632637.fastq FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content
Kmer Content

Basic Statistics

Measure	Value
Filename	ERR1632637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49293
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	97	0.19678250461525976	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	81	0.16432353478181486	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	72	0.14606536425050212	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	72	0.14606536425050212	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	67	0.13592193617755058	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	65	0.13186456494836996	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	56	0.1136063944170572	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	54	0.10954902318787657	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	53	0.10752033757328626	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	51	0.10346296634410565	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACCC	30	3.550127E-4	30.833332	32
GGTATCA	30	3.550127E-4	30.833332	1
CTGTAGT	40	0.0019052059	23.125002	4
CTTATAC	60	3.6339643E-5	21.583332	37
TACCCTG	45	0.0037748266	20.555555	34
ATACCCT	45	0.0037748266	20.555555	33
CTCTTAT	215	0.0	18.930233	37
GTATCAA	50	0.0069420305	18.5	2
TGATACC	50	0.0069420305	18.5	31
TCTTATA	110	3.633977E-8	18.5	37
TCTCTTA	340	0.0	13.058823	37
GTCTCTT	430	0.0	10.325582	36
ACCTGTC	175	0.0010591045	9.514286	37
TGTCTCT	570	9.094947E-12	8.114036	37
CCTGTCT	455	3.0629963E-7	7.3186817	37
CTGTCTC	710	1.6916601E-10	6.7746477	37
GCTGTCT	305	0.0028850168	6.672131	36