FastQCFastQC Report
Fri 10 Feb 2017
ERR1632636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226841
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12650.5576593296626272No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9210.40601125898757273No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7710.339885646774613No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6750.2975652549583188No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6480.28566264475998604No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5610.24730978967646944No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5560.2451056026027041No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5460.24069722845517344No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5300.2336438298191244No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5280.23276215498961828No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5270.23232131757486518No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5150.2270312685978284No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5110.2252679189388162No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC5070.22350456927980392No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4880.21512865839949566No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4880.21512865839949566No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4840.21336530874048343No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4730.20851609717819972No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4690.20675274751918746No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4650.20498939786017517No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4540.2001401862978915No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4480.19749516180937307No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4180.18427003936678113No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4170.18382920195202807No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4090.18030250263400355No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4080.17986166521925048No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4040.17809831556023822No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3940.17368994141270758No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3920.17280826658320145No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3810.16795905502091774No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3640.16046481897011564No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3550.15649728223733805No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3510.15473393257832577No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3440.15164807067505431No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3430.15120723326030128No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3270.14415383462425224No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3240.14283132237999302No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3180.14018629789147463No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3160.13930462306196853No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3130.1379821108177093No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3010.13269206184067253No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2990.1318103870111664No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2980.13136954959641334No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2960.13048787476690724No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2940.12960619993740108No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2940.12960619993740108No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2910.12828368769314188No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2890.12740201286363578No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2850.1256386632046235No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2840.12519782578987043No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2820.12431615096036432No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2750.12123028905709286No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2680.1181444271538214No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2650.1168219149095622No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2610.11505856525054994No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2600.11461772783579688No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2600.11461772783579688No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2570.11329521559153767No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2560.11285437817678463No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2550.11241354076203156No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2510.11065019110301928No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2420.10668265437024171No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2390.10536014212598252No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2390.10536014212598252No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2310.10183344280795799No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2310.10183344280795799No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2280.10051093056369881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG200.001838823237.05
GCCATAC200.001838823237.01
TCAATGC200.001838823237.032
AACCTCT200.001838823237.036
GCTAAAC303.5904546E-430.8333343
CCGGGAC250.005487160329.69
GGGCTAA250.005487160329.61
TGTATAA250.005487160329.610
GGTCAAA250.005487160329.627
GTGTATA250.005487160329.69
ATAAGAG250.005487160329.613
GTATAAG250.005487160329.611
TAAGAGA250.005487160329.614
TTTTACG250.005487160329.64
GCTTTTT250.005487160329.61
AGAGACA250.005487160329.616
GGTATCA4850.027.0824741
GTATTAG556.2245635E-726.909091
TATTAGC556.2245635E-726.909092
GATTGCA358.8473706E-426.428572
Produced by FastQC (version 0.11.5)