##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632636.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226841
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.781230024554645	34.0	31.0	34.0	31.0	34.0
2	32.93782869939738	34.0	33.0	34.0	31.0	34.0
3	33.0126696673	34.0	33.0	34.0	31.0	34.0
4	36.39588081519655	37.0	37.0	37.0	35.0	37.0
5	36.327185120855574	37.0	37.0	37.0	35.0	37.0
6	36.368121283189545	37.0	37.0	37.0	35.0	37.0
7	36.38572392116064	37.0	37.0	37.0	35.0	37.0
8	36.35162073875534	37.0	37.0	37.0	35.0	37.0
9	38.10644460216628	39.0	38.0	39.0	37.0	39.0
10	38.10897060055281	39.0	38.0	39.0	37.0	39.0
11	38.193003028553036	39.0	38.0	39.0	37.0	39.0
12	38.126035416877905	39.0	38.0	39.0	37.0	39.0
13	38.17727394959465	39.0	38.0	39.0	37.0	39.0
14	39.604555613844056	41.0	39.0	41.0	37.0	41.0
15	39.61926635837437	41.0	39.0	41.0	37.0	41.0
16	39.55067646501294	41.0	39.0	41.0	37.0	41.0
17	39.55504075541899	41.0	39.0	41.0	37.0	41.0
18	39.57865200735317	41.0	39.0	41.0	37.0	41.0
19	39.581235314603624	41.0	39.0	41.0	37.0	41.0
20	39.57111368756089	41.0	39.0	41.0	37.0	41.0
21	39.51030457456985	40.0	39.0	41.0	37.0	41.0
22	39.468508779277116	40.0	39.0	41.0	37.0	41.0
23	39.4435397481055	40.0	39.0	41.0	37.0	41.0
24	39.42695544456249	40.0	39.0	41.0	37.0	41.0
25	39.395603087625254	40.0	39.0	41.0	37.0	41.0
26	39.28690580626959	40.0	39.0	41.0	36.0	41.0
27	39.118986426616	40.0	39.0	41.0	36.0	41.0
28	39.09613782340935	40.0	39.0	41.0	36.0	41.0
29	39.07247807935955	40.0	39.0	41.0	36.0	41.0
30	39.03535956903735	40.0	39.0	41.0	35.0	41.0
31	38.97350567137334	40.0	39.0	41.0	35.0	41.0
32	38.92442724198888	40.0	38.0	41.0	35.0	41.0
33	38.85173756067025	40.0	38.0	41.0	35.0	41.0
34	38.828426078178104	40.0	38.0	41.0	35.0	41.0
35	38.73734906829012	40.0	38.0	41.0	35.0	41.0
36	38.68161840231704	40.0	38.0	41.0	35.0	41.0
37	38.59247226030568	40.0	38.0	41.0	35.0	41.0
38	38.53183066553225	40.0	38.0	41.0	35.0	41.0
39	38.40358665320643	40.0	38.0	41.0	34.0	41.0
40	38.32103984729392	40.0	38.0	41.0	34.0	41.0
41	38.251819556429396	40.0	37.0	41.0	34.0	41.0
42	38.19205963648547	40.0	37.0	41.0	34.0	41.0
43	37.410939821284515	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	2.0
20	12.0
21	33.0
22	51.0
23	93.0
24	129.0
25	239.0
26	345.0
27	496.0
28	706.0
29	921.0
30	1304.0
31	1683.0
32	2264.0
33	2989.0
34	4457.0
35	7163.0
36	11661.0
37	23620.0
38	64356.0
39	104311.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.912436464307596	15.997989781388727	12.73491123738654	28.354662516917134
2	22.648903857768214	19.727915147614407	30.744442142293497	26.878738852323874
3	22.58013322106674	18.874894750067227	28.907913472432234	29.637058556433804
4	15.82606318963503	16.618688861361044	35.60820133926407	31.94704660973986
5	17.818648304318884	32.011849709708564	32.315586688473424	17.853915297499128
6	31.02569641290596	34.748568380495584	16.389012568274694	17.83672263832376
7	28.63239008821157	27.090340811405344	19.49471215521004	24.782556945173052
8	26.30300518865637	29.187404393385673	21.49038313179716	23.019207286160793
9	27.92616854977716	12.216927275051688	17.23542040460058	42.62148377057058
10	20.472930378547087	21.79985099695381	27.72558752606451	30.00163109843459
11	40.3595469954726	19.141601385992832	18.133847055867324	22.36500456266724
12	21.61514012017228	23.354684558787874	26.966906335274487	28.06326898576536
13	37.670438765478906	17.06173046318787	19.254014926754863	26.01381584457836
14	23.892947042201364	20.440308409855362	23.003337139229682	32.66340740871359
15	31.218783200567803	23.16997368200634	19.04197213025864	26.569270987167222
16	24.361998051498627	23.88236694424729	23.102084720134368	28.653550284119717
17	27.539554137038717	23.19333806498825	20.672629727430227	28.594478070542806
18	26.776464572101162	19.99550345836952	22.825679661084195	30.402352308445124
19	28.889398301012605	22.180293685885708	22.74456557676963	26.185742436332056
20	31.429062647405008	19.352321670244795	21.59089406236086	27.62772161998933
21	28.161575729255294	21.004580300739285	21.34358427268439	29.49025969732103
22	28.71923505891792	22.300201462698542	20.591956480530417	28.38860699785312
23	29.55462195987498	20.707015045780967	21.635859478665672	28.102503515678386
24	28.60417649366737	20.021072028425195	22.47389140411125	28.90086007379618
25	29.450584329993255	21.81792533095869	21.957229954020658	26.7742603850274
26	29.31216138176079	22.273310380398605	21.444976878077597	26.969551359763006
27	27.501642119369958	21.136831525165203	24.69174443773392	26.669781917730923
28	26.716510683694743	23.278860523450344	20.75109878725627	29.253530005598634
29	28.10118100343412	23.671646659995325	20.89701597153954	27.330156365031012
30	27.674891223367908	21.351078508735196	23.626681243690513	27.34734902420638
31	29.347869212355793	21.527854312051172	22.037021526090964	27.087254949502075
32	26.071124708496257	21.229407382263346	22.318275796703418	30.381192112536976
33	26.614236403472034	21.295092157061553	23.75099739465088	28.33967404481553
34	26.568389312337715	21.432192593049756	23.209208211919364	28.790209882693162
35	25.913304914014663	23.691484343659212	23.705150303516557	26.690060438809564
36	26.912683333259864	21.48994229438241	24.50791523578189	27.08945913657584
37	27.829184318531482	21.21530058499125	23.69368853073298	27.26182656574429
38	26.563540100775434	20.371537773153882	25.261747215009635	27.803174911061053
39	25.84144841540991	19.150858971702647	26.5582500517984	28.449442561089043
40	25.854673537852506	20.34949590241623	27.5294148764994	26.26641568323187
41	23.444174553982748	19.45062841373473	28.38684364819411	28.718353384088413
42	21.32330575160575	20.45044767039468	30.169590153455506	28.056656424544062
43	20.534647616612517	21.893308528881462	28.537610044039656	29.034433810466364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	4.0
4	6.0
5	6.0
6	8.5
7	11.0
8	11.5
9	12.0
10	19.5
11	27.0
12	27.0
13	47.0
14	67.0
15	132.0
16	197.0
17	196.5
18	196.0
19	196.0
20	204.0
21	212.0
22	169.5
23	127.0
24	122.5
25	118.0
26	118.0
27	117.5
28	117.0
29	141.0
30	165.0
31	202.0
32	239.0
33	239.0
34	500.5
35	762.0
36	727.0
37	692.0
38	1446.0
39	2200.0
40	2200.0
41	3217.0
42	4234.0
43	5650.0
44	7066.0
45	12072.5
46	17079.0
47	17079.0
48	20154.0
49	23229.0
50	26349.0
51	29469.0
52	30365.0
53	31261.0
54	31261.0
55	28580.0
56	25899.0
57	24601.0
58	23303.0
59	21423.5
60	19544.0
61	19544.0
62	17651.0
63	15758.0
64	12256.5
65	8755.0
66	7547.5
67	6340.0
68	6340.0
69	5256.0
70	4172.0
71	3389.0
72	2606.0
73	1997.5
74	1389.0
75	1389.0
76	1091.0
77	793.0
78	617.0
79	441.0
80	320.0
81	199.0
82	199.0
83	149.5
84	100.0
85	64.5
86	29.0
87	22.0
88	15.0
89	15.0
90	10.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	226841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.91811004183547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.32724572578765	29.21120961378234
2	7.538323698712521	5.415246802826649
3	2.886704223277735	3.1105487984976263
4	1.697411539452852	2.438712578413955
5	1.0628766400333838	1.9088260058807711
6	0.72535807651239	1.5632094727143682
7	0.5596671453293568	1.4071530278917832
8	0.46516194754347856	1.3366190415312929
9	0.3473372853688771	1.1228128953760563
>10	2.7296046737115995	20.15200074060686
>50	0.35715600721676055	8.931806860311848
>100	0.28597027381960555	19.40742634708893
>500	0.01595542300281061	3.4367684854148943
>1k	0.0012273402309854315	0.5576593296626272
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1265	0.5576593296626272	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	921	0.40601125898757273	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	771	0.339885646774613	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	675	0.2975652549583188	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	648	0.28566264475998604	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	561	0.24730978967646944	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	556	0.2451056026027041	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	546	0.24069722845517344	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	530	0.2336438298191244	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	528	0.23276215498961828	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	527	0.23232131757486518	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	515	0.2270312685978284	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	511	0.2252679189388162	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	507	0.22350456927980392	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	488	0.21512865839949566	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	488	0.21512865839949566	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	484	0.21336530874048343	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	473	0.20851609717819972	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	469	0.20675274751918746	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	465	0.20498939786017517	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	454	0.2001401862978915	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	448	0.19749516180937307	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	418	0.18427003936678113	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	417	0.18382920195202807	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	409	0.18030250263400355	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	408	0.17986166521925048	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	404	0.17809831556023822	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	394	0.17368994141270758	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	392	0.17280826658320145	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	381	0.16795905502091774	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	364	0.16046481897011564	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	355	0.15649728223733805	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	351	0.15473393257832577	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	344	0.15164807067505431	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	343	0.15120723326030128	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	327	0.14415383462425224	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	324	0.14283132237999302	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	318	0.14018629789147463	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	316	0.13930462306196853	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	313	0.1379821108177093	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	301	0.13269206184067253	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	299	0.1318103870111664	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	298	0.13136954959641334	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	296	0.13048787476690724	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	294	0.12960619993740108	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	294	0.12960619993740108	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	291	0.12828368769314188	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	289	0.12740201286363578	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	285	0.1256386632046235	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	284	0.12519782578987043	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	282	0.12431615096036432	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	275	0.12123028905709286	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	268	0.1181444271538214	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	265	0.1168219149095622	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	261	0.11505856525054994	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	260	0.11461772783579688	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	260	0.11461772783579688	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	257	0.11329521559153767	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	256	0.11285437817678463	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	255	0.11241354076203156	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	251	0.11065019110301928	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	242	0.10668265437024171	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	239	0.10536014212598252	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	239	0.10536014212598252	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	231	0.10183344280795799	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	231	0.10183344280795799	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	228	0.10051093056369881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.408374147530649E-4	0.0
9	0.0	0.0	0.0	4.408374147530649E-4	0.0
10	0.0	0.0	0.0	4.408374147530649E-4	0.0
11	0.0	0.0	0.0	4.408374147530649E-4	0.0
12	0.0	0.0	0.0	4.408374147530649E-4	0.0
13	0.0	0.0	0.0	4.408374147530649E-4	0.0
14	0.0	0.0	0.0	8.816748295061298E-4	0.0
15	0.0	0.0	0.0	0.0013225122442591948	0.0
16	0.0	0.0	0.0	0.0013225122442591948	0.0
17	0.0	0.0	0.0	0.0017633496590122597	0.0
18	0.0	0.0	0.0	0.0017633496590122597	0.0
19	0.0	0.0	0.0	0.002204187073765325	0.0
20	0.0	0.0	0.0	0.0035266993180245194	0.0
21	0.0	0.0	0.0	0.006171723806542909	0.0
22	0.0	0.0	0.0	0.0088167482950613	0.0
23	0.0	0.0	0.0	0.009257585709814364	0.0
24	0.0	0.0	0.0	0.011902610198332752	0.0
25	0.0	0.0	0.0	0.014547634686851142	0.0
26	0.0	0.0	0.0	0.021160195908147116	0.0
27	0.0	0.0	0.0	0.055104676844133116	0.0
28	0.0	0.0	0.0	0.17677580331597903	0.0
29	0.0	0.0	0.0	0.35046574472868663	0.0
30	0.0	0.0	0.0	0.5819053874740457	0.0
31	0.0	0.0	0.0	1.1082652606892052	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	20	0.0018388232	37.0	5
GCCATAC	20	0.0018388232	37.0	1
TCAATGC	20	0.0018388232	37.0	32
AACCTCT	20	0.0018388232	37.0	36
GCTAAAC	30	3.5904546E-4	30.833334	3
CCGGGAC	25	0.0054871603	29.6	9
GGGCTAA	25	0.0054871603	29.6	1
TGTATAA	25	0.0054871603	29.6	10
GGTCAAA	25	0.0054871603	29.6	27
GTGTATA	25	0.0054871603	29.6	9
ATAAGAG	25	0.0054871603	29.6	13
GTATAAG	25	0.0054871603	29.6	11
TAAGAGA	25	0.0054871603	29.6	14
TTTTACG	25	0.0054871603	29.6	4
GCTTTTT	25	0.0054871603	29.6	1
AGAGACA	25	0.0054871603	29.6	16
GGTATCA	485	0.0	27.082474	1
GTATTAG	55	6.2245635E-7	26.90909	1
TATTAGC	55	6.2245635E-7	26.90909	2
GATTGCA	35	8.8473706E-4	26.42857	2
>>END_MODULE