FastQCFastQC Report
Fri 10 Feb 2017
ERR1632635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78081
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3010.3854971119734635No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2810.35988268592871503No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1910.24461776872734725No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1810.23181055570497305No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1780.22796839179826078No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1760.22540694919378595No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1720.22028406398483624No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1610.20619612966022463No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1550.19851180184680012No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1490.1908274740333756No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1460.18698531012666333No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1410.18058170361547624No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1400.17930098231323882No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1400.17930098231323882No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1370.17545881840652655No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1340.1716166544998143RNA PCR Primer, Index 17 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1260.16137088408191494No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1210.15496727757072784No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1170.14984439236177816No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1150.1472829497573033No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1150.1472829497573033No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1130.14472150715282847No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1120.14344078585059106No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1110.14216006454835362No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1110.14216006454835362No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1080.13831790064164137No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1060.13575645803716654No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1060.13575645803716654No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1060.13575645803716654No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1030.13191429413045427No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1020.13063357282821686No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1020.13063357282821686No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC990.1267914089215046No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA960.12294924501479233No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC950.12166852371255492No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC940.1203878024103175No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC940.1203878024103175No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG910.11654563850360523No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC910.11654563850360523No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA860.11014203199241812No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC850.10886131069018071No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG850.10886131069018071No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC840.10758058938794329No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG810.10373842548123102No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA790.1011769828767562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC200.001831445437.029
GGTATCA1400.030.3928571
GCGGTAA1400.027.7523
GGTAATA1400.027.7525
CGCGGTA1450.026.79310222
AGCCGCG1450.026.79310219
ACGGCTG358.7952317E-426.4285731
CGGTAAT1500.025.89999824
CCGCGGT1500.025.89999821
CCAGCAG1550.025.06451414
TAATACG1500.024.66666427
GCAGCCG1600.024.28125217
CGTGCCA1600.024.28125210
GCCGCGG1600.024.28125220
GTAATAC1600.024.28125226
GCCAGCA1700.023.94117713
AACTCCG1650.023.5454565
CGGCTGT400.001915191823.12500232
CAGCCGC1700.022.85294218
GTAACGT652.630055E-622.7692326
Produced by FastQC (version 0.11.5)