##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78081
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.968417412686826	34.0	33.0	34.0	31.0	34.0
2	33.11215276443693	34.0	33.0	34.0	31.0	34.0
3	33.18744636979547	34.0	33.0	34.0	31.0	34.0
4	36.48745533484458	37.0	37.0	37.0	35.0	37.0
5	36.463992520587595	37.0	37.0	37.0	35.0	37.0
6	36.51349239891907	37.0	37.0	37.0	35.0	37.0
7	36.52367413327186	37.0	37.0	37.0	35.0	37.0
8	36.49508843380592	37.0	37.0	37.0	35.0	37.0
9	38.275329465555004	39.0	39.0	39.0	37.0	39.0
10	38.303582177482355	39.0	39.0	39.0	37.0	39.0
11	38.38018211856918	39.0	39.0	39.0	37.0	39.0
12	38.31709378722096	39.0	39.0	39.0	37.0	39.0
13	38.365043992776734	39.0	39.0	39.0	37.0	39.0
14	39.87816498251816	41.0	40.0	41.0	38.0	41.0
15	39.904445383640066	41.0	40.0	41.0	38.0	41.0
16	39.83743804510701	41.0	40.0	41.0	38.0	41.0
17	39.85375443449751	41.0	40.0	41.0	38.0	41.0
18	39.86002996887847	41.0	40.0	41.0	38.0	41.0
19	39.860439799695186	41.0	40.0	41.0	38.0	41.0
20	39.84311164047592	41.0	40.0	41.0	38.0	41.0
21	39.79736427555999	41.0	40.0	41.0	38.0	41.0
22	39.775041303262	41.0	40.0	41.0	38.0	41.0
23	39.732149946850065	41.0	40.0	41.0	38.0	41.0
24	39.71529565451262	41.0	40.0	41.0	38.0	41.0
25	39.66624402863693	41.0	40.0	41.0	37.0	41.0
26	39.58696738002843	41.0	40.0	41.0	37.0	41.0
27	39.44803473316172	41.0	39.0	41.0	37.0	41.0
28	39.43573980866024	41.0	39.0	41.0	37.0	41.0
29	39.41172628424329	41.0	39.0	41.0	37.0	41.0
30	39.364096259013074	41.0	39.0	41.0	36.0	41.0
31	39.3062973066431	41.0	39.0	41.0	36.0	41.0
32	39.2502017136051	41.0	39.0	41.0	36.0	41.0
33	39.15983401851923	41.0	39.0	41.0	35.0	41.0
34	39.13154288495281	41.0	39.0	41.0	35.0	41.0
35	39.054289776001845	40.0	39.0	41.0	35.0	41.0
36	39.00176739539709	40.0	39.0	41.0	35.0	41.0
37	38.95354823836785	40.0	39.0	41.0	35.0	41.0
38	38.87290121796596	40.0	39.0	41.0	35.0	41.0
39	38.77779485406181	40.0	38.0	41.0	35.0	41.0
40	38.70455040278685	40.0	38.0	41.0	35.0	41.0
41	38.63828588260908	40.0	38.0	41.0	35.0	41.0
42	38.572969096194974	40.0	38.0	41.0	35.0	41.0
43	37.82945915139406	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	3.0
21	9.0
22	14.0
23	40.0
24	40.0
25	76.0
26	105.0
27	135.0
28	164.0
29	253.0
30	361.0
31	498.0
32	606.0
33	885.0
34	1214.0
35	2095.0
36	3254.0
37	6111.0
38	16714.0
39	45500.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.768407166916404	16.19984375200113	12.973706791665066	28.0580422894174
2	22.267901282002022	19.806354939101702	31.345653872260858	26.58008990663542
3	21.95156312034938	19.128853370218106	28.999372446561907	29.92021106287061
4	15.719573263662095	16.475198831982173	34.69986296282066	33.10536494153507
5	17.4447048577759	32.237035898618096	32.65839320705422	17.659866036551787
6	32.54568973245732	34.34766460470537	15.756714181426979	17.34993148141033
7	29.20941074012884	27.302416721097323	19.40036628629244	24.087806252481396
8	26.445614169900487	30.086704832161477	20.88984516079456	22.57783583714348
9	28.00169055211895	12.321819648826219	17.18599915472394	42.49049064433088
10	20.4633649671495	21.989984759416505	28.274484189495524	29.272166083938473
11	40.137805612120744	18.94955238790487	18.282296589439174	22.630345410535213
12	22.440798657804077	23.63443091148935	26.55959836579962	27.365172064906957
13	36.79000012807213	17.045119811477825	19.85118018468001	26.313699875770034
14	23.839346319847337	20.433908377198037	22.963332949116943	32.76341235383768
15	30.766767843649546	23.271986782956162	19.208258090956825	26.752987282437466
16	25.15336637594293	23.37188304453068	22.69566219694932	28.77908838257707
17	27.363891343604717	23.2527759634226	21.26637722365236	28.11695546932032
18	26.843918494896325	20.190571329772926	23.070913538504886	29.89459663682586
19	28.875142480244875	22.166724299125267	22.773786196385803	26.184347024244055
20	31.243196168081866	19.808916381706176	21.90289571086436	27.0449917393476
21	28.302660058144745	21.555820237958017	21.19209538812259	28.949424315774646
22	28.521663400827347	22.283269937628873	20.91930175074602	28.27576491079776
23	29.185077035386332	21.546855188842358	21.52508292670432	27.742984849066993
24	28.465311663528897	20.39804818073539	22.314007248882568	28.82263290685314
25	28.925090611032132	21.94644023514043	22.07707380796865	27.051395345858786
26	29.128725298087883	22.275585609815447	21.680050204275048	26.91563888782162
27	27.330592589746544	21.480257681126012	24.4438467745034	26.745302954624044
28	26.61338866049359	23.15416042315032	21.012794405809352	29.219656510546738
29	27.503489965548596	23.672852550556474	21.72871761376007	27.09493987013486
30	27.217889115149653	21.851666858774863	23.709993468321358	27.220450557754127
31	28.80470280862182	21.637786401301213	22.187215839961066	27.370294950115902
32	25.911553386867485	21.454643255081262	22.832699376288726	29.801103981762527
33	26.181785581639577	21.384203583458202	24.135192940664183	28.298817894238038
34	26.129276008247842	21.601926204838566	24.060911105134412	28.207886681779176
35	26.029379746673325	23.145195374034657	24.069876154250075	26.755548725041944
36	26.412315416042315	21.0281630614362	25.33650952216288	27.223012000358604
37	27.18330963998924	20.553015458306117	24.534777986962258	27.728896914742386
38	25.663093454233426	19.842215135564352	26.338033580512544	28.156657829689678
39	25.09445319604001	18.6357756688567	27.71480898041777	28.55496215468552
40	24.829343886476867	19.72182733315403	29.022425430002173	26.42640335036693
41	22.894173998796123	19.37475186024769	29.53855611480386	28.19251802615233
42	20.553015458306117	20.076587133873797	31.489094658111448	27.881302749708638
43	19.679563530180197	21.28686876448816	30.090546996068184	28.943020709263457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	4.0
6	3.5
7	3.0
8	2.5
9	2.0
10	6.0
11	10.0
12	10.0
13	18.5
14	27.0
15	53.0
16	79.0
17	72.5
18	66.0
19	66.0
20	80.5
21	95.0
22	81.5
23	68.0
24	77.5
25	87.0
26	87.0
27	98.0
28	109.0
29	147.0
30	185.0
31	223.0
32	261.0
33	261.0
34	382.0
35	503.0
36	568.5
37	634.0
38	905.5
39	1177.0
40	1177.0
41	1493.0
42	1809.0
43	2336.0
44	2863.0
45	4264.5
46	5666.0
47	5666.0
48	6605.5
49	7545.0
50	8442.5
51	9340.0
52	9632.0
53	9924.0
54	9924.0
55	9341.5
56	8759.0
57	8361.5
58	7964.0
59	7351.5
60	6739.0
61	6739.0
62	5987.0
63	5235.0
64	4157.0
65	3079.0
66	2644.0
67	2209.0
68	2209.0
69	1844.0
70	1479.0
71	1236.5
72	994.0
73	755.5
74	517.0
75	517.0
76	400.0
77	283.0
78	234.0
79	185.0
80	135.5
81	86.0
82	86.0
83	62.0
84	38.0
85	36.5
86	35.0
87	24.5
88	14.0
89	14.0
90	9.0
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	78081.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.379669830048286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0549015980204	47.65692037755664
2	6.052126454059804	6.703295295910657
3	2.340371406766726	3.8882698735928076
4	1.262690502069795	2.7970953240865253
5	0.7677898290973845	2.1259973617141172
6	0.5897180916260031	1.9595035924232527
7	0.43477255382622976	1.6854292337444448
8	0.3260794153696723	1.4446536289238099
9	0.23126199671607964	1.1526491720136782
>10	1.6928378159617028	18.409087998360675
>50	0.17344649753705974	6.340851167377467
>100	0.07400383894914549	5.836246974295924
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	301	0.3854971119734635	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	281	0.35988268592871503	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	191	0.24461776872734725	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	181	0.23181055570497305	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	178	0.22796839179826078	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	176	0.22540694919378595	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	172	0.22028406398483624	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	161	0.20619612966022463	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	155	0.19851180184680012	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	149	0.1908274740333756	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	146	0.18698531012666333	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	141	0.18058170361547624	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	140	0.17930098231323882	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	140	0.17930098231323882	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	137	0.17545881840652655	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	134	0.1716166544998143	RNA PCR Primer, Index 17 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	126	0.16137088408191494	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	121	0.15496727757072784	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	117	0.14984439236177816	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	115	0.1472829497573033	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	115	0.1472829497573033	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	113	0.14472150715282847	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	112	0.14344078585059106	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	111	0.14216006454835362	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	111	0.14216006454835362	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	108	0.13831790064164137	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	106	0.13575645803716654	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	106	0.13575645803716654	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	106	0.13575645803716654	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	103	0.13191429413045427	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	102	0.13063357282821686	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	102	0.13063357282821686	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	99	0.1267914089215046	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	96	0.12294924501479233	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	95	0.12166852371255492	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	94	0.1203878024103175	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	94	0.1203878024103175	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	91	0.11654563850360523	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	91	0.11654563850360523	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	86	0.11014203199241812	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	85	0.10886131069018071	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	85	0.10886131069018071	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	84	0.10758058938794329	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	81	0.10373842548123102	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	79	0.1011769828767562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00128072130223742	0.0
2	0.0	0.0	0.0	0.00128072130223742	0.0
3	0.0	0.0	0.0	0.00128072130223742	0.0
4	0.0	0.0	0.0	0.00128072130223742	0.0
5	0.0	0.0	0.0	0.00128072130223742	0.0
6	0.0	0.0	0.0	0.00128072130223742	0.0
7	0.0	0.0	0.0	0.00128072130223742	0.0
8	0.0	0.0	0.0	0.00128072130223742	0.0
9	0.0	0.0	0.0	0.00128072130223742	0.0
10	0.0	0.0	0.0	0.00128072130223742	0.0
11	0.0	0.0	0.0	0.00128072130223742	0.0
12	0.0	0.0	0.0	0.00128072130223742	0.0
13	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
14	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
15	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
16	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
17	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
18	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
19	0.00128072130223742	0.0	0.0	0.00128072130223742	0.0
20	0.00128072130223742	0.0	0.0	0.0038421639067122604	0.0
21	0.00128072130223742	0.0	0.0	0.00512288520894968	0.0
22	0.00128072130223742	0.0	0.0	0.008965049115661941	0.0
23	0.00128072130223742	0.0	0.0	0.01024577041789936	0.0
24	0.00128072130223742	0.0	0.0	0.019210819533561303	0.0
25	0.00128072130223742	0.0	0.0	0.023052983440273563	0.0
26	0.00128072130223742	0.0	0.0	0.026895147346985823	0.0
27	0.00128072130223742	0.0	0.0	0.053790294693971646	0.0
28	0.00128072130223742	0.0	0.0	0.2087575722646995	0.0
29	0.00128072130223742	0.0	0.0	0.43672596406296027	0.0
30	0.00128072130223742	0.0	0.0	0.7556255683200779	0.0
31	0.00128072130223742	0.0	0.0	1.506128251431206	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGC	20	0.0018314454	37.0	29
GGTATCA	140	0.0	30.392857	1
GCGGTAA	140	0.0	27.75	23
GGTAATA	140	0.0	27.75	25
CGCGGTA	145	0.0	26.793102	22
AGCCGCG	145	0.0	26.793102	19
ACGGCTG	35	8.7952317E-4	26.42857	31
CGGTAAT	150	0.0	25.899998	24
CCGCGGT	150	0.0	25.899998	21
CCAGCAG	155	0.0	25.064514	14
TAATACG	150	0.0	24.666664	27
GCAGCCG	160	0.0	24.281252	17
CGTGCCA	160	0.0	24.281252	10
GCCGCGG	160	0.0	24.281252	20
GTAATAC	160	0.0	24.281252	26
GCCAGCA	170	0.0	23.941177	13
AACTCCG	165	0.0	23.545456	5
CGGCTGT	40	0.0019151918	23.125002	32
CAGCCGC	170	0.0	22.852942	18
GTAACGT	65	2.630055E-6	22.76923	26
>>END_MODULE