FastQCFastQC Report
Fri 10 Feb 2017
ERR1632634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37931
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1630.42972766338878493No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1170.30845482586802353No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1090.28736389760354325No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1040.27418206743824314No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA990.26100023727294297No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA980.2583638712398829No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA960.25309113917376286No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC940.24781840710764283No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA920.24254567504152277No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA830.2188183807439825No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT790.20827291661174238No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG790.20827291661174238No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA780.20563655057868235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC740.19509108644644224No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA720.18981835438032216No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA690.18190925628114207No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT660.17400015818196196No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC650.17136379214890196No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC640.16872742611584193No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA640.16872742611584193No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC630.1660910600827819No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT620.16345469404972188No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT610.16081832801666185No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC590.15554559595054177No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC590.15554559595054177No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT580.15290922991748174No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA580.15290922991748174No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC570.1502728638844217No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC560.14763649785136168No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA550.14500013181830165No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC540.14236376578524162No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC540.14236376578524162No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC530.1397273997521816No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC510.13445466768606154No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA510.13445466768606154No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA510.13445466768606154No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT500.1318183016530015No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC490.12918193561994146No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC490.12918193561994146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT460.12127283752076139No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA460.12127283752076139No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC450.11863647148770136No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA450.11863647148770136No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG450.11863647148770136No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG440.11600010545464133No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT440.11600010545464133No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT430.11336373942158129No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT430.11336373942158129No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA420.11072737338852126No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA420.11072737338852126No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA420.11072737338852126No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG410.10809100735546123No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC410.10809100735546123No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG410.10809100735546123No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA400.1054546413224012No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG400.1054546413224012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT390.10281827528934118No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG390.10281827528934118No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG390.10281827528934118No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC390.10281827528934118No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT380.10018190925628115No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT380.10018190925628115No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG380.10018190925628115No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC380.10018190925628115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA800.032.3751
GTATCAA900.028.7777792
TATCGCG405.7922596E-527.757
TCGCGCG405.7922596E-527.759
ATCGCGC405.7922596E-527.758
ACTTAAC358.7114883E-426.4285712
CTTTCCA451.2905351E-424.66666817
TAATGAG400.001897129323.1254
GGGAACT400.001897129323.12520
TTAATGA400.001897129323.1253
GGGGAAC400.001897129323.12519
GTTAATG400.001897129323.1252
GAAGGAC400.001897129323.12528
TAATACG652.571087E-622.7692327
ACGCTTC502.6359083E-422.225
GTATCGC502.6359083E-422.26
GCGCGCC502.6359083E-422.211
CCTTTCC502.6359083E-422.216
CGCGCGC502.6359083E-422.210
CGCGCCT502.6359083E-422.212
Produced by FastQC (version 0.11.5)