##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632634.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37931
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97461179510163	34.0	33.0	34.0	31.0	34.0
2	33.10587645988769	34.0	33.0	34.0	31.0	34.0
3	33.1919538108671	34.0	33.0	34.0	31.0	34.0
4	36.47879043526403	37.0	37.0	37.0	35.0	37.0
5	36.44873586248715	37.0	37.0	37.0	35.0	37.0
6	36.50201682001529	37.0	37.0	37.0	35.0	37.0
7	36.50586591442356	37.0	37.0	37.0	35.0	37.0
8	36.47388679444254	37.0	37.0	37.0	35.0	37.0
9	38.27194115631014	39.0	39.0	39.0	37.0	39.0
10	38.30581845983497	39.0	39.0	39.0	37.0	39.0
11	38.36632306029369	39.0	39.0	39.0	37.0	39.0
12	38.31349028499117	39.0	39.0	39.0	37.0	39.0
13	38.38092852811685	39.0	39.0	39.0	37.0	39.0
14	39.84951622683293	41.0	40.0	41.0	38.0	41.0
15	39.85236350214864	41.0	40.0	41.0	38.0	41.0
16	39.787218897471725	41.0	40.0	41.0	38.0	41.0
17	39.818855289868445	41.0	40.0	41.0	38.0	41.0
18	39.83709894281722	41.0	40.0	41.0	38.0	41.0
19	39.812317102106455	41.0	40.0	41.0	38.0	41.0
20	39.795470723155205	41.0	40.0	41.0	38.0	41.0
21	39.74487885898078	41.0	40.0	41.0	38.0	41.0
22	39.70583427803116	41.0	40.0	41.0	38.0	41.0
23	39.653291502992275	41.0	40.0	41.0	37.0	41.0
24	39.63478421344019	41.0	40.0	41.0	37.0	41.0
25	39.58058052780048	41.0	40.0	41.0	37.0	41.0
26	39.491234082940075	41.0	39.0	41.0	37.0	41.0
27	39.33444939495399	41.0	39.0	41.0	36.0	41.0
28	39.30218027470934	41.0	39.0	41.0	36.0	41.0
29	39.276607524188655	41.0	39.0	41.0	36.0	41.0
30	39.21910838100762	41.0	39.0	41.0	35.0	41.0
31	39.141256492051355	41.0	39.0	41.0	35.0	41.0
32	39.062165511059554	41.0	39.0	41.0	35.0	41.0
33	38.99290817537107	40.0	39.0	41.0	35.0	41.0
34	38.94990904537186	40.0	39.0	41.0	35.0	41.0
35	38.860061690965175	40.0	38.0	41.0	35.0	41.0
36	38.78215707468825	40.0	38.0	41.0	35.0	41.0
37	38.7260288418444	40.0	38.0	41.0	35.0	41.0
38	38.65497877725343	40.0	38.0	41.0	35.0	41.0
39	38.549576863251694	40.0	38.0	41.0	35.0	41.0
40	38.43088766444333	40.0	38.0	41.0	35.0	41.0
41	38.37773852521684	40.0	37.0	41.0	35.0	41.0
42	38.31367483061348	40.0	37.0	41.0	35.0	41.0
43	37.54538504125913	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	4.0
22	9.0
23	13.0
24	23.0
25	40.0
26	63.0
27	72.0
28	119.0
29	137.0
30	180.0
31	273.0
32	361.0
33	482.0
34	726.0
35	1164.0
36	1760.0
37	3176.0
38	7728.0
39	21597.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.355039413672195	15.794468904062638	12.918193561994148	27.93229812027102
2	22.61474783158894	19.973109066462786	30.378845798950728	27.03329730299755
3	22.09538372307611	19.095199177453797	28.89984445440405	29.909572645066042
4	15.9157417415834	16.630196936542667	34.39139490126809	33.06266642060584
5	17.980016345469405	32.137301943001766	31.78402889457172	18.098652816957106
6	32.57757507052279	34.022303656639686	15.826105296459362	17.57401597637816
7	29.461390419445838	27.418206743824314	19.221744747040677	23.898658089689174
8	27.626479660436054	29.511481374073977	20.423927658116053	22.438111307373916
9	28.731117028288207	12.082465529514117	17.2550156863779	41.93140175581978
10	21.09883736257942	22.061110964646332	27.24947931770847	29.590572355065774
11	39.911945374495794	18.760380691255175	18.39656217869289	22.93111175555614
12	23.039202762911604	23.66929424481295	26.144841949856318	27.14666104241913
13	37.932034483667714	16.64074240067491	19.43265402968548	25.994569085971897
14	24.48129498299544	20.497745907041732	22.89156626506024	32.12939284490259
15	31.16711924283568	22.98120271018428	19.2533811394374	26.598296907542647
16	24.262476602251457	23.872294429358572	22.406474914977196	29.45875405341278
17	27.94284358440326	22.90211172919248	20.645382404893095	28.509662281511165
18	27.718752471593156	19.316653924230838	22.15074740977037	30.81384619440563
19	29.55366323060294	21.481110437373125	22.158656507869555	26.806569824154387
20	31.987029079117345	19.27710843373494	21.710474282249347	27.02538820489837
21	28.346207587461443	20.919564472331338	21.080382800347998	29.653845139859218
22	29.413935830850757	22.298383907621737	20.02056365505787	28.267116606469646
23	29.89639081490074	20.31583665076059	21.496928633571486	28.290843900767182
24	28.85238986580897	19.498563180511983	22.435474941340853	29.21357201233819
25	28.99738999762727	20.824655295141177	22.179747436134033	27.99820727109752
26	29.369117608288736	22.179747436134033	21.0672009701827	27.38393398539453
27	27.101842819857108	21.0672009701827	24.913659012417284	26.917297197542904
28	26.893569903245368	23.00756637051488	20.653291502992275	29.445572223247474
29	28.06675278795708	23.355566686878806	21.00656455142232	27.571115973741794
30	27.15720650655137	21.541746856133507	23.46893042630039	27.832116211014736
31	29.87002715457014	21.647201497455907	21.394110358282145	27.08866098969181
32	26.18702380638528	21.1858374416704	22.01629274208431	30.61084600986001
33	26.366296696633363	20.35538214125649	24.439113126466477	28.839208035643672
34	26.250296591178717	21.05138277398434	23.91447628588753	28.783844348949405
35	26.039387308533918	23.872294429358572	24.056840051672772	26.031478210434738
36	27.18620653291503	21.24383749439772	24.6948406316733	26.875115341013945
37	27.412934011758196	21.336110305554822	24.056840051672772	27.19411563101421
38	26.22393293084812	20.463473148611953	25.087659170599245	28.224934749940683
39	25.625477841343493	19.213835648941497	26.503387730352486	28.657298779362527
40	25.562205056550052	20.547836861669875	27.54738867944425	26.34256940233582
41	23.63765785241623	19.55919959927236	28.670480609527825	28.13266193878358
42	21.707837916216288	20.526745933405394	30.15211832010756	27.613297830270756
43	20.724473385884895	21.212201102001004	28.828662571511426	29.234662940602675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.5
4	3.0
5	3.0
6	4.0
7	5.0
8	6.0
9	7.0
10	7.0
11	7.0
12	7.0
13	17.0
14	27.0
15	52.5
16	78.0
17	72.5
18	67.0
19	67.0
20	74.0
21	81.0
22	64.5
23	48.0
24	44.5
25	41.0
26	41.0
27	44.5
28	48.0
29	62.5
30	77.0
31	102.0
32	127.0
33	127.0
34	167.5
35	208.0
36	213.0
37	218.0
38	300.5
39	383.0
40	383.0
41	567.5
42	752.0
43	967.5
44	1183.0
45	1981.5
46	2780.0
47	2780.0
48	3253.5
49	3727.0
50	4148.0
51	4569.0
52	4732.5
53	4896.0
54	4896.0
55	4439.0
56	3982.0
57	3938.5
58	3895.0
59	3507.0
60	3119.0
61	3119.0
62	2826.5
63	2534.0
64	1951.0
65	1368.0
66	1229.0
67	1090.0
68	1090.0
69	910.0
70	730.0
71	643.0
72	556.0
73	417.5
74	279.0
75	279.0
76	258.5
77	238.0
78	235.5
79	233.0
80	220.0
81	207.0
82	207.0
83	191.0
84	175.0
85	123.5
86	72.0
87	76.0
88	80.0
89	80.0
90	51.0
91	22.0
92	19.0
93	16.0
94	9.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	37931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.0665946059951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14355373512775	44.41222219292927
2	7.767700409596255	8.399462181329257
3	3.052467329822508	4.951095410086737
4	1.7554125219426564	3.7963670876064435
5	1.145894285157012	3.0977300888455352
6	0.7704310512970548	2.4992749993409085
7	0.5656329237370782	2.1407292188447444
8	0.3754632338599571	1.6240014763649786
9	0.2828164618685391	1.3761830692573358
>10	1.9650867953969184	20.671746065223694
>50	0.15603666861712503	5.731459755872505
>100	0.01950458357714063	1.2997284542985947
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	163	0.42972766338878493	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	117	0.30845482586802353	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	109	0.28736389760354325	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	104	0.27418206743824314	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	99	0.26100023727294297	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	98	0.2583638712398829	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	96	0.25309113917376286	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	94	0.24781840710764283	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	92	0.24254567504152277	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	83	0.2188183807439825	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	79	0.20827291661174238	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	79	0.20827291661174238	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	78	0.20563655057868235	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	74	0.19509108644644224	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	72	0.18981835438032216	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	69	0.18190925628114207	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	66	0.17400015818196196	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	65	0.17136379214890196	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	64	0.16872742611584193	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	64	0.16872742611584193	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	63	0.1660910600827819	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	62	0.16345469404972188	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	61	0.16081832801666185	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	59	0.15554559595054177	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	59	0.15554559595054177	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	58	0.15290922991748174	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	58	0.15290922991748174	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	57	0.1502728638844217	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	56	0.14763649785136168	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	55	0.14500013181830165	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	54	0.14236376578524162	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	54	0.14236376578524162	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	53	0.1397273997521816	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	51	0.13445466768606154	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	51	0.13445466768606154	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	51	0.13445466768606154	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	50	0.1318183016530015	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	49	0.12918193561994146	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	49	0.12918193561994146	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.12127283752076139	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	46	0.12127283752076139	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	45	0.11863647148770136	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	45	0.11863647148770136	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	45	0.11863647148770136	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	44	0.11600010545464133	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	44	0.11600010545464133	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	43	0.11336373942158129	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	43	0.11336373942158129	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	42	0.11072737338852126	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	42	0.11072737338852126	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	42	0.11072737338852126	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	41	0.10809100735546123	No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC	41	0.10809100735546123	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	41	0.10809100735546123	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	40	0.1054546413224012	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	40	0.1054546413224012	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.10281827528934118	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	39	0.10281827528934118	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	39	0.10281827528934118	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	39	0.10281827528934118	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	38	0.10018190925628115	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	38	0.10018190925628115	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	38	0.10018190925628115	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	38	0.10018190925628115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.00790909809918009	0.0
18	0.0	0.0	0.0	0.00790909809918009	0.0
19	0.0	0.0	0.0	0.00790909809918009	0.0
20	0.0	0.0	0.0	0.00790909809918009	0.0
21	0.0	0.0	0.0	0.01581819619836018	0.0
22	0.0	0.0	0.0	0.02900002636366033	0.0
23	0.0	0.0	0.0	0.03690912446284042	0.0
24	0.0	0.0	0.0	0.04481822256202051	0.0
25	0.0	0.0	0.0	0.04481822256202051	0.0
26	0.0	0.0	0.0	0.04745458859508054	0.0
27	0.0	0.0	0.0	0.08436371305792097	0.0
28	0.0	0.0	0.0	0.19245472041338219	0.0
29	0.0	0.0	0.0	0.4033640030581846	0.0
30	0.0	0.0	0.0	0.6248187498352271	0.0
31	0.0	0.0	0.0	1.3656376051250956	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	80	0.0	32.375	1
GTATCAA	90	0.0	28.777779	2
TATCGCG	40	5.7922596E-5	27.75	7
TCGCGCG	40	5.7922596E-5	27.75	9
ATCGCGC	40	5.7922596E-5	27.75	8
ACTTAAC	35	8.7114883E-4	26.42857	12
CTTTCCA	45	1.2905351E-4	24.666668	17
TAATGAG	40	0.0018971293	23.125	4
GGGAACT	40	0.0018971293	23.125	20
TTAATGA	40	0.0018971293	23.125	3
GGGGAAC	40	0.0018971293	23.125	19
GTTAATG	40	0.0018971293	23.125	2
GAAGGAC	40	0.0018971293	23.125	28
TAATACG	65	2.571087E-6	22.76923	27
ACGCTTC	50	2.6359083E-4	22.2	25
GTATCGC	50	2.6359083E-4	22.2	6
GCGCGCC	50	2.6359083E-4	22.2	11
CCTTTCC	50	2.6359083E-4	22.2	16
CGCGCGC	50	2.6359083E-4	22.2	10
CGCGCCT	50	2.6359083E-4	22.2	12
>>END_MODULE