FastQCFastQC Report
Fri 10 Feb 2017
ERR1632633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290329
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11670.4019577789335547No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11370.39162467407665097No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10770.3709584643628435No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9700.3341037237065536No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8950.30827096156429434No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8830.30413771962153285No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8570.29518236207888293No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8540.29414905159319255No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7940.2734828418793851No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7850.270382910422314No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7720.26590523165098906No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7050.24282796413723742No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6830.23525035390884133No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6660.22939492782326257No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6530.22491724905193763No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6510.2242283753948107No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5630.19391793448122646No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5480.1887513820527746No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5480.1887513820527746No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5440.18737363473852078No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5420.18668476108139387No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5290.18220708231006894No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5270.18151820865294202No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5260.18117377182437855No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5200.1791071508529978No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5130.17669609305305362No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC5020.17290728793885557No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4720.16257418308195187No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4610.15878537796775383No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4590.1580965043106269No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4550.15671875699637308No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4490.15465213602499234No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4380.15086333091079432No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4360.15017445725366738No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4320.14879670993941357No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4300.14810783628228663No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4200.14466346799665208No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4160.14328572068239825No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4140.14259684702527134No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4110.14156353653958098No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4090.14087466288245404No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4040.13915247873963676No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3990.13743029459681946No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3840.13226374216836762No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3830.13191930533980417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3790.1305415580255503No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3780.13019712119698687No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3720.12813050022560613No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3700.1274416265684792No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3690.12709718973991574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.12537500559709847No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3620.12468613193997156No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3600.12399725828284464No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3590.12365282145428119No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3530.12158620048290042No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3370.11607521122588513No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3340.11504190074019476No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3310.11400859025450438No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3290.11331971659737745No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT3280.11297527976881401No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3280.11297527976881401No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG3270.11263084294025054No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3260.11228640611168708No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3240.11159753245456017No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3190.1098753483117429No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3190.1098753483117429No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3170.10918647465461596No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3160.10884203782605252No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3140.10815316416892559No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3120.10746429051179869No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3100.10677541685467178No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3080.10608654319754485No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT3030.10436435905472757No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC3000.1033310485690372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC200.001839670937.022
TAGCCAT200.001839670937.025
AAACCCG200.001839670937.012
ACCCGGT200.001839670937.014
GATCTAG200.001839670937.021
ATGCCCC251.2313541E-436.99999637
GTTTTAA451.075241E-732.888891
TGTTTGA303.592912E-430.83333434
AACCTTG551.8457285E-830.2727286
GGTATCA6300.030.246031
GCCATGG250.00548966329.59999827
TCTAGCC250.00548966329.59999823
TTTTAAC502.7089482E-729.5999982
CTAGCCA250.00548966329.59999824
TGATCTA250.00548966329.59999820
ATACGGC652.9704097E-928.46153829
ATGTTTG358.8533695E-426.4285733
CGGGGTT509.0605645E-625.89999816
TTTAACC659.34142E-825.6153853
TACGGCT809.713403E-1025.437530
Produced by FastQC (version 0.11.5)