FastQCFastQC Report
Fri 10 Feb 2017
ERR1632632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21363
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT450.21064457239151807No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA450.21064457239151807No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA420.19660160089875017No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA330.15447268642044656No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA310.145110705425268No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA270.12638674343491083No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT250.11702476243973224No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT250.11702476243973224No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC240.11234377194214297No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT230.10766278144455367No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA220.10298179094696439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251.1935207E-437.01
GTATCAA400.001870140523.1252
CTCTTAT701.149675E-418.537
TCTCTTA1152.0162122E-514.4782637
GTCTCTT1502.4311904E-411.136
TGTCTCT1900.00213557838.76315835
Produced by FastQC (version 0.11.5)